FastQCFastQC Report
Thu 2 Feb 2017
SRR4062772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838113
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36360.1978115600074642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26960.14667215780531448No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21730.1182190648779482No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19880.10815439529561022No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19240.10467256365631493No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19010.10342128041094319No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18770.10211559354620744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG751.5080859E-619.2019755
CGTCTTA1401.8189894E-1218.28510915
TTAATAC1850.018.1625483
CTAGCGG4250.016.56415629
TAATACT2550.016.3140744
TTAGTAC908.584671E-616.0003413
TAGCGGC4400.015.9994730
TCTAGCG4400.015.9994728
ATAGCGT3800.015.1594546
TAATACC1901.8189894E-1215.1582174
TTTTCGG5650.015.00835229
TATACTG2350.014.9802645
CGCGGTT5900.014.91597710
GTATCAA25850.014.7941561
AATAGCG3900.014.7707515
TCGTTTA3500.014.62808730
GTATTAG3850.014.5465551
GACCGCG6100.014.4277137
TAGCGTA3900.014.3604527
CGAGCCG4800.014.33285815
Produced by FastQC (version 0.11.5)