##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062772_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1838113
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.224733735085927	32.0	32.0	32.0	32.0	32.0
2	31.26694767949522	32.0	32.0	32.0	32.0	32.0
3	31.35596560167955	32.0	32.0	32.0	32.0	32.0
4	31.459084397966826	32.0	32.0	32.0	32.0	32.0
5	31.375980149207365	32.0	32.0	32.0	32.0	32.0
6	34.905408427011835	36.0	36.0	36.0	36.0	36.0
7	34.93099064094536	36.0	36.0	36.0	36.0	36.0
8	34.86013155883234	36.0	36.0	36.0	32.0	36.0
9	34.976794136160294	36.0	36.0	36.0	36.0	36.0
10	34.82150553312011	36.0	36.0	36.0	32.0	36.0
11	35.00560792508404	36.0	36.0	36.0	36.0	36.0
12	34.900588810372376	36.0	36.0	36.0	32.0	36.0
13	34.965458053993416	36.0	36.0	36.0	36.0	36.0
14	34.906245698713846	36.0	36.0	36.0	32.0	36.0
15	34.87330213104418	36.0	36.0	36.0	32.0	36.0
16	34.88654451603356	36.0	36.0	36.0	32.0	36.0
17	34.85262277128773	36.0	36.0	36.0	32.0	36.0
18	34.853339266954755	36.0	36.0	36.0	32.0	36.0
19	34.846054622321915	36.0	36.0	36.0	32.0	36.0
20	34.83823192589357	36.0	36.0	36.0	32.0	36.0
21	34.83399170780034	36.0	36.0	36.0	32.0	36.0
22	34.81908293994983	36.0	36.0	36.0	32.0	36.0
23	34.7590975092391	36.0	36.0	36.0	32.0	36.0
24	34.73667124926487	36.0	36.0	36.0	32.0	36.0
25	34.717403119394724	36.0	36.0	36.0	32.0	36.0
26	34.654735046213155	36.0	36.0	36.0	32.0	36.0
27	34.63999764976364	36.0	36.0	36.0	32.0	36.0
28	34.61281814556559	36.0	36.0	36.0	32.0	36.0
29	34.57539716002226	36.0	36.0	36.0	32.0	36.0
30	34.55870286538423	36.0	36.0	36.0	32.0	36.0
31	34.554037755023764	36.0	36.0	36.0	32.0	36.0
32	34.5338648929636	36.0	36.0	36.0	32.0	36.0
33	34.49866792737987	36.0	36.0	36.0	32.0	36.0
34	34.49384613459564	36.0	36.0	36.0	32.0	36.0
35	34.467840116467265	36.0	36.0	36.0	32.0	36.0
36	34.444282805246466	36.0	36.0	36.0	32.0	36.0
37	34.435838819484985	36.0	36.0	36.0	32.0	36.0
38	34.00663724156241	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	11.0
21	28.0
22	133.0
23	449.0
24	1184.0
25	2972.0
26	6337.0
27	12329.0
28	21208.0
29	33780.0
30	50808.0
31	73652.0
32	106129.0
33	169930.0
34	418515.0
35	940646.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66113947012474	18.33942529198419	12.00184771880723	26.997587519083844
2	15.890970794505016	20.45886188716363	36.62076270610131	27.02940461223004
3	17.747167883584957	24.19149421172692	29.001916639510195	29.05942126517793
4	12.264805329401748	16.209574534643014	35.78712859167458	35.73849154428066
5	14.426370957607068	36.73925378907608	33.25606205929668	15.578313194020172
6	34.55513048171167	35.751085896833274	16.322944412527637	13.370839208927421
7	30.163733578222825	30.502941067663052	20.95312136174806	18.380203992366063
8	27.98949766820379	33.25910684479573	19.21990916549333	19.531486321507145
9	27.605155034036105	13.849475095318512	18.14501035881544	40.40035951182994
10	15.714596594309342	26.694793536804305	31.68581687612208	25.90479299276427
11	37.80508837436542	20.851203433235423	22.147154050008897	19.196554142390262
12	24.663975496569847	23.65397065463982	28.569400090310154	23.112653758480178
13	29.65268672712732	19.215226509838914	24.982345996703135	26.14974076633063
14	23.595569802057764	19.44419056074929	24.992709911039153	31.967529726153792
15	25.30453786029477	26.984031993680475	21.979769470103307	25.731660675921447
16	25.8965580462137	25.546470755606425	23.35917323907725	25.197797959102626
17	24.410087954331424	25.762072299145917	24.838244438726022	24.989595307796638
18	25.299151901977734	24.60490731527387	25.677148249318734	24.418792533429663
19	25.87033550168026	24.779651740671003	25.075716237249836	24.27429652039891
20	25.992526030771774	24.062176808498716	24.683411738016105	25.261885422713405
21	27.03311876534183	24.108269789871343	24.17116040807089	24.687451036715935
22	25.996225476888522	24.164727630999835	24.715020240866583	25.124026651245053
23	24.60871556862935	23.861971489239238	25.31411289730283	26.215200044828585
24	25.185937970081273	24.618780238211688	24.956137081887782	25.239144709819257
25	25.163578082522676	24.174357071627263	25.089208334852103	25.572856510997966
26	25.10091876882366	24.90087655159207	25.2644017339531	24.73380294563117
27	25.637556362180327	24.457378005257567	24.495134137636878	25.409931494925225
28	24.760447263035516	24.484512105621363	25.325211235653082	25.429829395690035
29	24.86375973620773	24.73487756193444	25.191160717540217	25.210201984317614
30	24.906629788266553	24.77812843932881	25.377112288526334	24.938129483878306
31	25.42063518401752	24.48184632827253	24.474556243277753	25.622962244432195
32	25.140130122576792	24.519656843730502	24.37450798726738	25.96570504642533
33	24.80930171322438	24.193779163740206	25.015056201658986	25.981862921376432
34	25.485157876583216	24.272337990101807	25.207318592491323	25.03518554082366
35	25.98316860824117	24.2095018097364	25.111350607933247	24.69597897408919
36	25.094485485930406	24.797224109725573	24.708872631878453	25.399417772465565
37	25.85118542766413	24.607301074525886	24.603166399454224	24.93834709835576
38	24.957959557393433	24.46076433904155	25.181232254200097	25.400043849364916
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	156.0
1	147.5
2	139.0
3	139.0
4	420.0
5	701.0
6	701.0
7	800.5
8	900.0
9	898.0
10	896.0
11	896.0
12	1299.0
13	1702.0
14	2395.5
15	3089.0
16	3089.0
17	4896.5
18	6704.0
19	6704.0
20	8169.5
21	9635.0
22	10787.5
23	11940.0
24	11940.0
25	14099.5
26	16259.0
27	16259.0
28	20963.5
29	25668.0
30	31684.0
31	37700.0
32	37700.0
33	48968.0
34	60236.0
35	60236.0
36	67557.5
37	74879.0
38	85927.5
39	96976.0
40	96976.0
41	105169.0
42	113362.0
43	128157.0
44	142952.0
45	142952.0
46	147828.5
47	152705.0
48	152705.0
49	163805.5
50	174906.0
51	176993.0
52	179080.0
53	179080.0
54	172493.0
55	165906.0
56	165906.0
57	160479.5
58	155053.0
59	139213.0
60	123373.0
61	123373.0
62	114783.0
63	106193.0
64	87407.0
65	68621.0
66	68621.0
67	56634.0
68	44647.0
69	44647.0
70	35588.5
71	26530.0
72	20557.0
73	14584.0
74	14584.0
75	10894.0
76	7204.0
77	7204.0
78	6977.0
79	6750.0
80	5253.0
81	3756.0
82	3756.0
83	3515.5
84	3275.0
85	3275.0
86	2082.5
87	890.0
88	735.0
89	580.0
90	580.0
91	335.5
92	91.0
93	70.0
94	49.0
95	49.0
96	32.0
97	15.0
98	15.0
99	13.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009303018911242127
2	0.0
3	0.0
4	4.352289549119124E-4
5	0.0
6	5.440361936398905E-5
7	2.7201809681994525E-4
8	0.002448162871379507
9	0.003536235258659288
10	7.072470517318576E-4
11	0.004352289549119124
12	1.6321085809196714E-4
13	1.6321085809196714E-4
14	5.440361936398905E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	5.440361936398905E-5
22	0.0
23	0.0
24	0.0
25	0.0
26	5.440361936398905E-5
27	5.440361936398905E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.088072387279781E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1838113.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.020342540542956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.26737784519277	43.61318281333151
2	12.417636779443244	13.664452582980239
3	3.626323127000841	5.985646218308374
4	1.5236524230648747	3.353275129190307
5	0.8118340951415712	2.2333695000390494
6	0.5035018946544235	1.6621708028219253
7	0.3550489242888504	1.3674439403116694
8	0.25064849360861413	1.1032612780493631
9	0.18580883985308044	0.9200939414199638
>10	0.9126690793367059	8.817613360431821
>50	0.0666866607089307	2.54828902528176
>100	0.0656772836485611	7.876534378129904
>500	0.009154386161387947	3.546217820231247
>1k	0.003980167896255629	3.3084492094728515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3636	0.1978115600074642	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2696	0.14667215780531448	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2173	0.1182190648779482	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1988	0.10815439529561022	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1924	0.10467256365631493	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1901	0.10342128041094319	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1877	0.10211559354620744	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.4403619363989045E-5	0.0	0.0	0.0
6	0.0	5.4403619363989045E-5	0.0	0.0	1.6321085809196714E-4
7	0.0	5.4403619363989045E-5	0.0	0.0	1.6321085809196714E-4
8	0.0	5.4403619363989045E-5	0.0	0.0	1.6321085809196714E-4
9	0.0	5.4403619363989045E-5	0.0	0.0	2.7201809681994525E-4
10	0.0	5.4403619363989045E-5	0.0	0.0	2.7201809681994525E-4
11	0.0	5.4403619363989045E-5	0.0	0.0	3.264217161839343E-4
12	0.0	5.4403619363989045E-5	0.0	0.0	4.3522895491191236E-4
13	0.0	5.4403619363989045E-5	0.0	0.0	4.896325742759014E-4
14	0.0	5.4403619363989045E-5	0.0	0.0	4.896325742759014E-4
15	0.0	5.4403619363989045E-5	0.0	0.0	4.896325742759014E-4
16	0.0	5.4403619363989045E-5	0.0	5.4403619363989045E-5	4.896325742759014E-4
17	0.0	5.4403619363989045E-5	0.0	5.4403619363989045E-5	4.896325742759014E-4
18	0.0	5.4403619363989045E-5	0.0	1.0880723872797809E-4	4.896325742759014E-4
19	0.0	5.4403619363989045E-5	0.0	1.0880723872797809E-4	5.440361936398905E-4
20	0.0	5.4403619363989045E-5	0.0	1.0880723872797809E-4	5.984398130038795E-4
21	0.0	5.4403619363989045E-5	0.0	1.6321085809196714E-4	6.528434323678686E-4
22	0.0	5.4403619363989045E-5	0.0	3.264217161839343E-4	6.528434323678686E-4
23	0.0	5.4403619363989045E-5	0.0	4.3522895491191236E-4	6.528434323678686E-4
24	0.0	5.4403619363989045E-5	0.0	8.160542904598357E-4	6.528434323678686E-4
25	0.0	1.0880723872797809E-4	0.0	8.704579098238247E-4	6.528434323678686E-4
26	0.0	1.0880723872797809E-4	0.0	0.001033668767915792	7.616506710958466E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	75	1.5080859E-6	19.201975	5
CGTCTTA	140	1.8189894E-12	18.285109	15
TTAATAC	185	0.0	18.162548	3
CTAGCGG	425	0.0	16.564156	29
TAATACT	255	0.0	16.314074	4
TTAGTAC	90	8.584671E-6	16.000341	3
TAGCGGC	440	0.0	15.99947	30
TCTAGCG	440	0.0	15.99947	28
ATAGCGT	380	0.0	15.159454	6
TAATACC	190	1.8189894E-12	15.158217	4
TTTTCGG	565	0.0	15.008352	29
TATACTG	235	0.0	14.980264	5
CGCGGTT	590	0.0	14.915977	10
GTATCAA	2585	0.0	14.794156	1
AATAGCG	390	0.0	14.770751	5
TCGTTTA	350	0.0	14.628087	30
GTATTAG	385	0.0	14.546555	1
GACCGCG	610	0.0	14.427713	7
TAGCGTA	390	0.0	14.360452	7
CGAGCCG	480	0.0	14.332858	15
>>END_MODULE