FastQCFastQC Report
Thu 2 Feb 2017
SRR4062771_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062771_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602124
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35080.21895933148745042No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23090.14412117913469868No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22860.14268558488606375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22490.14037615065999884No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18870.11778114552930984No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18400.11484753989079498No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17360.10835615720131526No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17130.10692056295268032No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16750.10454871158537041No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16720.10436146016163544No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16610.10367487160794046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG453.5426003E-421.328885
TAGCGTA3150.018.2841827
AATAGCG3250.018.2115825
ATAGCGT3200.017.9962446
CAAGACG4900.017.9576784
TCTAGCG3850.017.86918828
AAGACGG5000.017.2763945
CGCAAGA4900.016.985062
AACCGCG2650.016.9042427
TAGCGGC4200.016.76206830
TAGGACG3600.016.4430644
GCGCAAG5200.016.323141
GACGGAC5100.016.3123577
CGTCGTA3950.016.20218310
CTAGCGG4250.016.18839529
GCGAGAA3800.015.99965720
CGTATAT3500.015.99965710
CCAATAG3800.015.9991593
TATACCG703.703413E-415.9966615
GAACCGC3150.015.7427466
Produced by FastQC (version 0.11.5)