##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062771_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1602124
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26158150055801	32.0	32.0	32.0	32.0	32.0
2	30.878322776514178	32.0	32.0	32.0	32.0	32.0
3	30.932583245741277	32.0	32.0	32.0	32.0	32.0
4	31.00993368802914	32.0	32.0	32.0	32.0	32.0
5	30.91337998806584	32.0	32.0	32.0	32.0	32.0
6	34.57549041147876	36.0	36.0	36.0	32.0	36.0
7	34.51210580454447	36.0	36.0	36.0	32.0	36.0
8	34.49177404495532	36.0	36.0	36.0	32.0	36.0
9	34.64090607218917	36.0	36.0	36.0	32.0	36.0
10	34.356810084612675	36.0	36.0	36.0	32.0	36.0
11	34.618958956984606	36.0	36.0	36.0	32.0	36.0
12	34.439083991001944	36.0	36.0	36.0	32.0	36.0
13	34.51726083623989	36.0	36.0	36.0	32.0	36.0
14	34.43153338942554	36.0	36.0	36.0	32.0	36.0
15	34.36702839480589	36.0	36.0	36.0	32.0	36.0
16	34.395105497452136	36.0	36.0	36.0	32.0	36.0
17	34.31556234099233	36.0	36.0	36.0	32.0	36.0
18	34.34724653023112	36.0	36.0	36.0	32.0	36.0
19	34.33824223343512	36.0	36.0	36.0	32.0	36.0
20	34.32006823441881	36.0	36.0	36.0	32.0	36.0
21	34.303023361487625	36.0	36.0	36.0	32.0	36.0
22	34.27563284739508	36.0	36.0	36.0	32.0	36.0
23	34.21524613575478	36.0	36.0	36.0	32.0	36.0
24	34.19554603763504	36.0	36.0	36.0	32.0	36.0
25	34.17687332566019	36.0	36.0	36.0	32.0	36.0
26	34.1348865630875	36.0	36.0	36.0	32.0	36.0
27	34.129521809797495	36.0	36.0	36.0	32.0	36.0
28	34.11212615253251	36.0	36.0	36.0	32.0	36.0
29	34.077121995550904	36.0	36.0	36.0	32.0	36.0
30	34.055646753934155	36.0	36.0	36.0	32.0	36.0
31	34.065865688298786	36.0	36.0	36.0	32.0	36.0
32	34.02353438310642	36.0	36.0	36.0	32.0	36.0
33	33.99557961805703	36.0	36.0	36.0	32.0	36.0
34	34.002140283773294	36.0	36.0	36.0	32.0	36.0
35	33.96851679395602	36.0	36.0	36.0	32.0	36.0
36	33.919043095291	36.0	36.0	36.0	32.0	36.0
37	33.91156864262691	36.0	36.0	36.0	32.0	36.0
38	33.313618671213966	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	5.0
10	14.0
11	16.0
12	6.0
13	1.0
14	159.0
15	327.0
16	490.0
17	563.0
18	711.0
19	925.0
20	1253.0
21	1847.0
22	2812.0
23	4428.0
24	6593.0
25	10204.0
26	14949.0
27	21594.0
28	30147.0
29	40622.0
30	55194.0
31	75015.0
32	102054.0
33	154122.0
34	343809.0
35	734258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.83524191910512	17.06403786967458	11.157368495055382	25.943351716164916
2	16.780058379974445	19.22404845263091	37.68162554629742	26.31426762109723
3	19.32432862663969	23.10052481330018	28.286981171768826	29.288165388291297
4	12.403570610438063	14.860940066001739	36.219312530780314	36.51617679277988
5	14.446536809641719	36.63836876240933	33.97077998478267	14.944314443166279
6	34.86037250642898	35.52311936683894	16.49614261104037	13.12036551569171
7	30.775083576552127	30.174630677775255	20.546536972169445	18.503748773503176
8	28.293653592523405	33.4520719122612	19.030858506765103	19.223415988450295
9	27.208278536113735	14.042839514418093	18.491135802022196	40.25774614744598
10	15.590746493545726	26.6235237501381	31.955281667943105	25.83044808837307
11	38.13335780128032	21.01871548271764	21.882146887577274	18.965779828424765
12	24.538383083055468	23.7335573745729	28.900071531740494	22.827988010631138
13	29.58928361853917	19.230980520437186	25.22523478603944	25.954501074984204
14	23.156829599592502	19.84733777453732	25.110364251970076	31.885468373900107
15	24.981036236240737	27.685034827771567	22.121893766712304	25.212035169275392
16	25.627751729614022	25.915089575372967	24.0987997730262	24.35835892198681
17	23.923598213254504	26.371289709357313	25.239496939371143	24.46561513801704
18	24.841670026978203	24.8213208958112	26.56447990492087	23.772529172289726
19	25.425839506820175	25.098750820842792	25.46628864203264	24.009121030304392
20	25.552628014384275	24.51762673163436	25.27753901775688	24.652206236224483
21	26.65356242657735	24.386433178987108	24.543109787231916	24.41689460720363
22	25.47994304665443	24.73581675055071	25.268771227199778	24.515468975595077
23	23.99873906041274	24.467221813006404	25.8957664889699	25.638272637610953
24	24.554780923748428	25.23216064842675	25.583054707261592	24.630003720563227
25	24.627119109630602	24.817149149859038	25.578892432009948	24.976839308500413
26	24.182225560098313	25.791523001691203	25.968696798697977	24.057554639512503
27	25.038592890418666	25.07791924862935	25.213314281897574	24.670173579054406
28	24.130515929045558	25.051340726397935	25.95256553823183	24.86557780632468
29	24.158274800430476	25.29158249830831	25.797841128426143	24.752301572835073
30	24.177179555840187	25.311641546213952	26.141250036671053	24.369928861274804
31	24.839765597105668	25.13158321827084	25.043757037954396	24.9848941466691
32	24.42475677551688	25.196034002114832	25.08336360764985	25.295845614718438
33	23.97027217835694	24.90560243095978	25.79555193785772	25.32857345282556
34	24.924575120558078	25.148680493780915	25.777923435866224	24.14882094979478
35	25.398302846879783	24.88127856446791	25.830550628987947	23.889867959664365
36	24.014785090555872	25.59211528425279	25.512583932419574	24.880515692771763
37	25.21679584111543	25.301441726676188	25.14869210870629	24.333070323502092
38	24.239176436660355	25.23417398349827	25.634482595033504	24.892166984807872
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	106.5
2	161.0
3	161.0
4	402.5
5	644.0
6	644.0
7	828.5
8	1013.0
9	1026.0
10	1039.0
11	1039.0
12	1403.0
13	1767.0
14	2587.0
15	3407.0
16	3407.0
17	4956.0
18	6505.0
19	6505.0
20	8277.0
21	10049.0
22	11326.5
23	12604.0
24	12604.0
25	15272.5
26	17941.0
27	17941.0
28	22757.5
29	27574.0
30	33590.5
31	39607.0
32	39607.0
33	49751.0
34	59895.0
35	59895.0
36	66567.0
37	73239.0
38	82765.5
39	92292.0
40	92292.0
41	97770.0
42	103248.0
43	114849.5
44	126451.0
45	126451.0
46	129326.5
47	132202.0
48	132202.0
49	139305.5
50	146409.0
51	147704.0
52	148999.0
53	148999.0
54	141516.0
55	134033.0
56	134033.0
57	128677.5
58	123322.0
59	110367.0
60	97412.0
61	97412.0
62	91561.5
63	85711.0
64	71594.0
65	57477.0
66	57477.0
67	48055.0
68	38633.0
69	38633.0
70	31041.5
71	23450.0
72	18387.0
73	13324.0
74	13324.0
75	10024.0
76	6724.0
77	6724.0
78	6449.5
79	6175.0
80	4826.5
81	3478.0
82	3478.0
83	3483.5
84	3489.0
85	3489.0
86	2329.0
87	1169.0
88	1010.5
89	852.0
90	852.0
91	628.5
92	405.0
93	357.5
94	310.0
95	310.0
96	288.5
97	267.0
98	267.0
99	531.0
100	795.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11853015122424981
2	0.05555125570804757
3	0.013794188215144394
4	0.002808771356024877
5	1.872514237349918E-4
6	2.4966856497998904E-4
7	0.0
8	3.1208570622498635E-4
9	3.1208570622498635E-4
10	4.3691998871498084E-4
11	0.0017476799548599234
12	0.0023718513673098964
13	0.014980113898799345
14	0.009737074034219573
15	0.024030599379323947
16	0.011047834000364517
17	0.0205352394696041
18	0.005617542712049754
19	0.0069907198194396935
20	0.0060544627007647345
21	0.006116879842009732
22	0.0069283026781946965
23	0.008988068339279606
24	0.01348210250891941
25	0.016977462418639257
26	0.01916206236221416
27	0.009050485480524603
28	0.0051182055820897755
29	0.010985416859119518
30	0.0026839370735348826
31	0.0059920455595197375
32	0.00661621697196971
33	0.007677308373134664
34	0.012421011107754454
35	0.016540542429924275
36	0.014980113898799345
37	0.009737074034219573
38	0.0068658855369497
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1602124.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.16281060601492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.32144379352154	44.54915224771208
2	12.852678417870726	14.43685087525778
3	3.8112689340156054	6.421547259291205
4	1.4140359617819709	3.176649356466199
5	0.7156580683312773	2.0096684275178007
6	0.4139527487546734	1.3949249896888802
7	0.2795052285762734	1.0988459451144108
8	0.1924929177211326	0.8648754624776946
9	0.13663574361582714	0.6906462651636941
>10	0.7095642152644175	7.284662226768565
>50	0.06842482202338254	2.677803124528896
>100	0.07147596099019182	8.653259457491718
>500	0.008724596761548693	3.330038126168391
>1k	0.004138590771503867	3.4110762363526463
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3508	0.21895933148745042	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2309	0.14412117913469868	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2286	0.14268558488606375	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2249	0.14037615065999884	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1887	0.11778114552930984	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1840	0.11484753989079498	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1736	0.10835615720131526	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1713	0.10692056295268032	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1675	0.10454871158537041	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1672	0.10436146016163544	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1661	0.10367487160794046	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	6.241714124499726E-5	0.0
4	0.0	0.0	0.0	6.241714124499726E-5	0.0
5	0.0	6.241714124499726E-5	0.0	6.241714124499726E-5	0.0
6	0.0	6.241714124499726E-5	0.0	6.241714124499726E-5	0.0
7	0.0	6.241714124499726E-5	0.0	6.241714124499726E-5	0.0
8	0.0	6.241714124499726E-5	0.0	6.241714124499726E-5	0.0
9	0.0	6.241714124499726E-5	0.0	6.241714124499726E-5	0.0
10	0.0	6.241714124499726E-5	0.0	6.241714124499726E-5	0.0
11	0.0	6.241714124499726E-5	0.0	1.872514237349918E-4	0.0
12	0.0	6.241714124499726E-5	0.0	1.872514237349918E-4	6.241714124499726E-5
13	0.0	6.241714124499726E-5	0.0	1.872514237349918E-4	6.241714124499726E-5
14	0.0	6.241714124499726E-5	0.0	1.872514237349918E-4	6.241714124499726E-5
15	0.0	6.241714124499726E-5	0.0	2.4966856497998904E-4	1.2483428248999452E-4
16	0.0	6.241714124499726E-5	0.0	3.1208570622498635E-4	1.872514237349918E-4
17	0.0	6.241714124499726E-5	0.0	3.1208570622498635E-4	1.872514237349918E-4
18	0.0	6.241714124499726E-5	0.0	5.617542712049754E-4	2.4966856497998904E-4
19	0.0	6.241714124499726E-5	0.0	5.617542712049754E-4	2.4966856497998904E-4
20	0.0	6.241714124499726E-5	0.0	7.490056949399672E-4	2.4966856497998904E-4
21	0.0	6.241714124499726E-5	0.0	0.0011235085424099508	2.4966856497998904E-4
22	0.0	6.241714124499726E-5	0.0	0.001185925683654948	2.4966856497998904E-4
23	0.0	6.241714124499726E-5	0.0	0.0018100970961049208	2.4966856497998904E-4
24	0.0	6.241714124499726E-5	0.0	0.0024966856497998908	2.4966856497998904E-4
25	0.0	6.241714124499726E-5	0.0	0.0027463542147798795	2.4966856497998904E-4
26	0.0	6.241714124499726E-5	0.0	0.0033705256272298523	3.1208570622498635E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	45	3.5426003E-4	21.32888	5
TAGCGTA	315	0.0	18.284182	7
AATAGCG	325	0.0	18.211582	5
ATAGCGT	320	0.0	17.996244	6
CAAGACG	490	0.0	17.957678	4
TCTAGCG	385	0.0	17.869188	28
AAGACGG	500	0.0	17.276394	5
CGCAAGA	490	0.0	16.98506	2
AACCGCG	265	0.0	16.904242	7
TAGCGGC	420	0.0	16.762068	30
TAGGACG	360	0.0	16.443064	4
GCGCAAG	520	0.0	16.32314	1
GACGGAC	510	0.0	16.312357	7
CGTCGTA	395	0.0	16.202183	10
CTAGCGG	425	0.0	16.188395	29
GCGAGAA	380	0.0	15.999657	20
CGTATAT	350	0.0	15.999657	10
CCAATAG	380	0.0	15.999159	3
TATACCG	70	3.703413E-4	15.996661	5
GAACCGC	315	0.0	15.742746	6
>>END_MODULE