FastQCFastQC Report
Thu 2 Feb 2017
SRR4062771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602124
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35880.22395270278705018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25820.16116105869458294No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24850.1551065959938182No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22680.1415620763436538No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19690.12289935111139963No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19270.12027783117910973No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18750.11703213983436987No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17720.11060317428613516No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17630.11004142001493017No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17330.10816890577758026No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16260.10149027166436554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG4850.024.08127829
TCTAGCG5000.023.35883728
TAGCGGC5150.022.67848230
ACGATTA453.5387994E-421.33227232
AGCGGCG5850.020.2383131
GCGGCGC6100.019.4088732
AGTATCG507.1909727E-419.202648
AGGGGCG3850.018.28480532
AAGACGG4500.018.1358265
AACGGAT450.00884315517.7807777
GGTCGTA1302.6011548E-1017.22991229
TTAGGAC3350.017.1942483
TAAACGC3700.016.86402728
CGCAAGA4750.016.8417932
CGGTCCA7550.016.31961610
ATCGTTT2950.016.27037829
CGCCGGT7250.016.1130647
TAGATCG600.002440228816.00225
ACCGTCG3300.016.00228
TTATACA3100.016.0007024
Produced by FastQC (version 0.11.5)