FastQCFastQC Report
Thu 2 Feb 2017
SRR4062770_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062770_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957314
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37500.3917210027221998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26760.2795321075425618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14570.15219666692433204No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14180.14812276849602116No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13810.14425778793582877No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13700.14310873966117701No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13530.14133293778216968No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13390.13987051270534015No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG13070.13652782681544404No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12700.13266284625525168No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12450.13005137290377033No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12390.12942461929941482No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12270.12817111209070378No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11990.1252462619370447No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11240.11741184188260069No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11210.11709846508042294No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT10890.11375577919052683No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT10550.11020417543251221No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10300.10759270208103089No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10250.10707040741073462No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC9940.10383218045489775No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA9850.10289205004836448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG453.539723E-421.3301647
GTATTGA507.153972E-419.2162191
ATACGTA507.1943394E-419.20015725
ATATCCG507.204461E-419.1961465
GTGTAAG1251.4551915E-1017.9351391
ATTCGTT550.001365778917.45195232
GTTATAT1302.564775E-1017.2453251
CGCAAGA3900.017.2363132
CAAGACG4000.017.1965484
TCTAGCG3150.016.75941528
CACGTTT1456.91216E-1116.54926728
CTAGCGG3100.016.51367629
AAGACGG4100.016.3869535
TACCGTC2250.016.3531277
TAGCGGC3150.016.25155430
GCTTATA600.002427784316.0135151
AGTTCGG600.00244137316.0001321
TACGTAG600.00244137316.0001326
TGGCGAT2900.015.99762430
ACCGTCG2200.015.99762258
Produced by FastQC (version 0.11.5)