##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062770_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	957314
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22149159001122	32.0	32.0	32.0	32.0	32.0
2	30.927430289330356	32.0	32.0	32.0	32.0	32.0
3	30.989942693828777	32.0	32.0	32.0	32.0	32.0
4	31.01799931892775	32.0	32.0	32.0	32.0	32.0
5	31.003713515105808	32.0	32.0	32.0	32.0	32.0
6	34.618975592125466	36.0	36.0	36.0	32.0	36.0
7	34.532746831238235	36.0	36.0	36.0	32.0	36.0
8	34.532747875827575	36.0	36.0	36.0	32.0	36.0
9	34.60419883131345	36.0	36.0	36.0	32.0	36.0
10	34.40362096449023	36.0	36.0	36.0	32.0	36.0
11	34.61691984030318	36.0	36.0	36.0	32.0	36.0
12	34.46891093204528	36.0	36.0	36.0	32.0	36.0
13	34.53863935970852	36.0	36.0	36.0	32.0	36.0
14	34.44362142410954	36.0	36.0	36.0	32.0	36.0
15	34.419459028072296	36.0	36.0	36.0	32.0	36.0
16	34.420923542327806	36.0	36.0	36.0	32.0	36.0
17	34.35075534255218	36.0	36.0	36.0	32.0	36.0
18	34.38472016496155	36.0	36.0	36.0	32.0	36.0
19	34.35914130577846	36.0	36.0	36.0	32.0	36.0
20	34.335002935296046	36.0	36.0	36.0	32.0	36.0
21	34.30269483158086	36.0	36.0	36.0	32.0	36.0
22	34.27339723434526	36.0	36.0	36.0	32.0	36.0
23	34.23885266485187	36.0	36.0	36.0	32.0	36.0
24	34.229876508648154	36.0	36.0	36.0	32.0	36.0
25	34.20181883895984	36.0	36.0	36.0	32.0	36.0
26	34.16732440975479	36.0	36.0	36.0	32.0	36.0
27	34.16587034139269	36.0	36.0	36.0	32.0	36.0
28	34.154180342082114	36.0	36.0	36.0	32.0	36.0
29	34.11302874500947	36.0	36.0	36.0	32.0	36.0
30	34.075743173086366	36.0	36.0	36.0	32.0	36.0
31	34.10026908621413	36.0	36.0	36.0	32.0	36.0
32	34.05009328182811	36.0	36.0	36.0	32.0	36.0
33	34.00451053677268	36.0	36.0	36.0	32.0	36.0
34	33.99716707370831	36.0	36.0	36.0	32.0	36.0
35	33.95723451239614	36.0	36.0	36.0	32.0	36.0
36	33.91286871392249	36.0	36.0	36.0	32.0	36.0
37	33.90371811129891	36.0	36.0	36.0	32.0	36.0
38	33.26124866031417	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	2.0
11	12.0
12	8.0
13	5.0
14	277.0
15	458.0
16	557.0
17	665.0
18	781.0
19	995.0
20	1254.0
21	1562.0
22	2137.0
23	2988.0
24	4125.0
25	5631.0
26	8205.0
27	11780.0
28	16468.0
29	22792.0
30	30599.0
31	41731.0
32	57787.0
33	88832.0
34	204698.0
35	452957.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.78813018640192	18.019027178994797	11.73489131958172	25.457951315021553
2	16.2921953111037	20.35011512681718	37.98286110260449	25.374828459474628
3	18.304067663454546	24.8170721515073	29.407819408111923	27.471040776926227
4	12.016981323260422	15.737809037415113	37.1384966055112	35.106713033813264
5	13.94161163421824	37.263740005891485	33.79288300390467	15.001765355985603
6	32.70918794245119	36.94564659895603	17.177421292393575	13.16774416619921
7	29.309192177279346	31.29673231562476	21.332812431448826	18.061263075647073
8	27.77245036101083	32.960623077951595	20.03871256183982	19.228213999197756
9	27.538775237331194	14.247288240406473	19.368920477336545	38.84501604492579
10	15.988776873506882	26.38646455846545	31.66908490362046	25.955673664407207
11	36.90182953199331	21.5174230696108	22.861837638175942	18.718909760219955
12	24.611116160080897	24.54279837583347	28.803258361355116	22.042827102730516
13	28.93703933931267	19.481813860434503	26.098716549047467	25.482430251205358
14	23.51803699644912	20.76341348499363	25.421033882245275	30.29751563631197
15	24.939975300541423	27.66381153184705	23.036110942779587	24.36010222483194
16	25.240856238442976	26.15507568872034	24.41073536632505	24.193332706511633
17	23.18861929792118	26.1124675984115	26.04152627284305	24.65738683082427
18	24.311342465638944	24.822383863961868	27.52185663053232	23.34441703986687
19	25.162784526877598	25.334631500542702	26.25100940509607	23.251574567483626
20	25.117078832209817	24.655658174729776	25.923337522368506	24.3039254706919
21	26.967346179801808	24.302745143922095	24.84565225112666	23.884256425149438
22	25.167041351875376	24.800783078157384	25.660121515009692	24.372054054957545
23	23.68314659562799	24.472720624722506	26.255373605996503	25.58875917365301
24	24.117937124159518	25.980998508130064	25.820841882681357	24.080222485029065
25	24.39968490248275	24.69493211194738	26.266449636634892	24.63893334893498
26	23.595803788124943	25.93211715208395	27.009796802796636	23.46228225699447
27	24.626607621727953	25.37553395867481	25.650387521585564	24.34747089801167
28	23.489905542706353	25.172315091647185	26.832473586013382	24.505305779633076
29	23.574302978638052	25.32824357302845	26.606847357336278	24.490606090997225
30	23.616213805113013	25.744842434264477	26.814849945105053	23.824093815517458
31	24.382172125795886	25.238883694692692	25.517385467973863	24.86155871153756
32	23.43765996343693	25.821363280229825	25.44403238443458	25.296944371898668
33	23.546728762051615	25.143616471156044	26.234882270534882	25.07477249625746
34	24.319188301749996	25.388031587865896	26.664667787292768	23.62811232309134
35	25.42646106270503	25.184395829415575	26.030109279341403	23.359033828538
36	23.650053385653365	26.03005058577957	25.65353024386065	24.66636578470642
37	24.81454358552202	25.878443388391332	25.463183989686943	23.843829036399704
38	23.858776385148474	25.693174909272486	26.122628914550038	24.325419791028995
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	796.0
1	581.5
2	367.0
3	367.0
4	754.0
5	1141.0
6	1141.0
7	1347.5
8	1554.0
9	1463.0
10	1372.0
11	1372.0
12	1717.5
13	2063.0
14	2636.0
15	3209.0
16	3209.0
17	4736.0
18	6263.0
19	6263.0
20	7319.0
21	8375.0
22	9140.5
23	9906.0
24	9906.0
25	11625.0
26	13344.0
27	13344.0
28	16916.0
29	20488.0
30	24335.0
31	28182.0
32	28182.0
33	34600.0
34	41018.0
35	41018.0
36	43842.0
37	46666.0
38	52266.0
39	57866.0
40	57866.0
41	58709.0
42	59552.0
43	67003.5
44	74455.0
45	74455.0
46	75055.0
47	75655.0
48	75655.0
49	79061.0
50	82467.0
51	82209.5
52	81952.0
53	81952.0
54	77325.5
55	72699.0
56	72699.0
57	70605.0
58	68511.0
59	61577.0
60	54643.0
61	54643.0
62	53226.5
63	51810.0
64	42640.5
65	33471.0
66	33471.0
67	28082.5
68	22694.0
69	22694.0
70	18229.5
71	13765.0
72	10772.0
73	7779.0
74	7779.0
75	5928.0
76	4077.0
77	4077.0
78	4111.5
79	4146.0
80	3227.0
81	2308.0
82	2308.0
83	2397.0
84	2486.0
85	2486.0
86	1609.0
87	732.0
88	661.0
89	590.0
90	590.0
91	399.0
92	208.0
93	193.0
94	178.0
95	178.0
96	155.0
97	132.0
98	132.0
99	263.0
100	394.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12618639234357798
2	0.05672120119417453
3	0.011072647010280849
4	0.002820391219599839
5	0.0
6	5.222946702962665E-4
7	0.0
8	2.0891786811850656E-4
9	2.0891786811850656E-4
10	5.222946702962665E-4
11	0.002089178681185066
12	0.0024025554833628253
13	0.013475202493643673
14	0.009087927263155037
15	0.021727458284324683
16	0.010863729142162342
17	0.019324902800961857
18	0.005536323505140424
19	0.00699874858196997
20	0.005536323505140424
21	0.006267536043555197
22	0.006894289647910717
23	0.009296845131273542
24	0.013161825691465913
25	0.015668840108887992
26	0.01723572411977679
27	0.008043337922562503
28	0.005849700307318184
29	0.010236975537806822
30	0.0032382269558368522
31	0.005118487768903411
32	0.00668537177979221
33	0.008043337922562503
34	0.011594941680577115
35	0.01399749716393994
36	0.01337074355958442
37	0.008461173658799517
38	0.005431864571081171
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	957314.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.23521016988197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.14695611043122	38.65905272462956
2	11.71965361007412	11.305999100002822
3	3.3100200540848226	4.789785389259165
4	1.4593060993070903	2.815597456090726
5	0.7568793231977056	1.8254116613839677
6	0.5006782945931778	1.449019366032001
7	0.33324292092348573	1.1251829629858785
8	0.22274852730397574	0.859545762363117
9	0.18724695156858742	0.8128706456322472
>10	1.0703170402086308	9.83035361014213
>50	0.1292123764388746	4.395686117995414
>100	0.14549724610063317	14.634603612727009
>500	0.013898244394507445	4.493253715570122
>1k	0.004343201373283576	3.003637875185874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3750	0.3917210027221998	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2676	0.2795321075425618	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1457	0.15219666692433204	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1418	0.14812276849602116	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1381	0.14425778793582877	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1370	0.14310873966117701	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1353	0.14133293778216968	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1339	0.13987051270534015	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1307	0.13652782681544404	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1270	0.13266284625525168	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1245	0.13005137290377033	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1239	0.12942461929941482	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1227	0.12817111209070378	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1199	0.1252462619370447	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1124	0.11741184188260069	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1121	0.11709846508042294	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1089	0.11375577919052683	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1055	0.11020417543251221	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1030	0.10759270208103089	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1025	0.10707040741073462	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	994	0.10383218045489775	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	985	0.10289205004836448	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0445893405925329E-4
2	0.0	0.0	0.0	0.0	1.0445893405925329E-4
3	0.0	0.0	0.0	0.0	1.0445893405925329E-4
4	0.0	0.0	0.0	0.0	1.0445893405925329E-4
5	0.0	0.0	0.0	0.0	1.0445893405925329E-4
6	0.0	0.0	0.0	0.0	2.0891786811850658E-4
7	0.0	0.0	0.0	0.0	2.0891786811850658E-4
8	0.0	0.0	0.0	0.0	2.0891786811850658E-4
9	0.0	0.0	0.0	1.0445893405925329E-4	2.0891786811850658E-4
10	0.0	0.0	0.0	1.0445893405925329E-4	2.0891786811850658E-4
11	0.0	0.0	0.0	1.0445893405925329E-4	2.0891786811850658E-4
12	0.0	0.0	0.0	1.0445893405925329E-4	4.1783573623701317E-4
13	0.0	0.0	0.0	1.0445893405925329E-4	4.1783573623701317E-4
14	0.0	0.0	0.0	1.0445893405925329E-4	4.1783573623701317E-4
15	0.0	0.0	0.0	1.0445893405925329E-4	5.222946702962664E-4
16	0.0	0.0	0.0	1.0445893405925329E-4	5.222946702962664E-4
17	0.0	0.0	0.0	1.0445893405925329E-4	5.222946702962664E-4
18	0.0	0.0	0.0	1.0445893405925329E-4	5.222946702962664E-4
19	0.0	0.0	0.0	1.0445893405925329E-4	5.222946702962664E-4
20	0.0	0.0	0.0	2.0891786811850658E-4	5.222946702962664E-4
21	0.0	0.0	0.0	4.1783573623701317E-4	6.267536043555197E-4
22	0.0	0.0	0.0	8.356714724740263E-4	6.267536043555197E-4
23	0.0	0.0	0.0	0.0020891786811850655	7.31212538414773E-4
24	0.0	0.0	0.0	0.0033426858898961053	7.31212538414773E-4
25	0.0	0.0	0.0	0.003969439494251625	7.31212538414773E-4
26	0.0	0.0	0.0	0.005118487768903411	7.31212538414773E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	45	3.539723E-4	21.330164	7
GTATTGA	50	7.153972E-4	19.216219	1
ATACGTA	50	7.1943394E-4	19.200157	25
ATATCCG	50	7.204461E-4	19.196146	5
GTGTAAG	125	1.4551915E-10	17.935139	1
ATTCGTT	55	0.0013657789	17.451952	32
GTTATAT	130	2.564775E-10	17.245325	1
CGCAAGA	390	0.0	17.236313	2
CAAGACG	400	0.0	17.196548	4
TCTAGCG	315	0.0	16.759415	28
CACGTTT	145	6.91216E-11	16.549267	28
CTAGCGG	310	0.0	16.513676	29
AAGACGG	410	0.0	16.386953	5
TACCGTC	225	0.0	16.353127	7
TAGCGGC	315	0.0	16.251554	30
GCTTATA	60	0.0024277843	16.013515	1
AGTTCGG	60	0.002441373	16.00013	21
TACGTAG	60	0.002441373	16.00013	26
TGGCGAT	290	0.0	15.997624	30
ACCGTCG	220	0.0	15.9976225	8
>>END_MODULE