FastQCFastQC Report
Thu 2 Feb 2017
SRR4062770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957314
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT43720.45669445970705536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT32290.33729789807732885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18860.1970095496357517No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15370.1605533816490723No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15090.15762853149541323No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14270.14906289890255442No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13940.14561575407859909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13690.14300428072711774No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13370.13966159483722165No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13050.13631890894732554No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12830.13402081239802197No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12610.1317227158487184No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12070.1260819334095187No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA12050.1258730155414002No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11790.12315708325585963No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11630.12148574031091157No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11570.12085898670655605No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT11420.11929210269566726No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11420.11929210269566726No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10970.11459145066300086No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10240.10696594847667536No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG10130.10581690020202358No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT9610.10038503563094242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA200.00375347531.9995322
ACCGTCG2400.022.0008268
ACCTCGC400.00448196720.0007518
ATACCGT2750.019.782566
CCGTCGT2600.019.6930479
TACCGTC2800.019.42937
CGTCGTA2650.019.3204710
ATAACAC507.1929186E-419.2007223
TCAGATA2950.018.9827732
TCTAGCG3150.018.79337528
TCGAACT601.13805836E-418.66639119
AACCGCG2450.018.28647
CTAGCGG3300.017.9391329
CGTGATC450.00884997417.77751513
GTCGTAG2900.017.65491311
ATTGTCG550.001364076917.45526
CGAACTC652.100177E-417.23051620
TTAGGAC2150.017.1169223
GAACCGC2650.016.9062946
AGAACCG2750.016.873365
Produced by FastQC (version 0.11.5)