##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062770_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	957314
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14537340935158	32.0	32.0	32.0	32.0	32.0
2	31.271110628278706	32.0	32.0	32.0	32.0	32.0
3	31.347494134630853	32.0	32.0	32.0	32.0	32.0
4	31.455843119394473	32.0	32.0	32.0	32.0	32.0
5	31.368559323273242	32.0	32.0	32.0	32.0	32.0
6	34.87238147567047	36.0	36.0	36.0	36.0	36.0
7	34.90904447234659	36.0	36.0	36.0	36.0	36.0
8	34.83619794550169	36.0	36.0	36.0	32.0	36.0
9	34.957313901186026	36.0	36.0	36.0	32.0	36.0
10	34.80178186049718	36.0	36.0	36.0	32.0	36.0
11	34.98573717714355	36.0	36.0	36.0	36.0	36.0
12	34.87074460417376	36.0	36.0	36.0	32.0	36.0
13	34.93390152029533	36.0	36.0	36.0	36.0	36.0
14	34.870575380700586	36.0	36.0	36.0	32.0	36.0
15	34.84038883793614	36.0	36.0	36.0	32.0	36.0
16	34.849566599882586	36.0	36.0	36.0	32.0	36.0
17	34.827365942626976	36.0	36.0	36.0	32.0	36.0
18	34.822465774030256	36.0	36.0	36.0	32.0	36.0
19	34.80518408797949	36.0	36.0	36.0	32.0	36.0
20	34.78616002690862	36.0	36.0	36.0	32.0	36.0
21	34.770338676756005	36.0	36.0	36.0	32.0	36.0
22	34.76053102743718	36.0	36.0	36.0	32.0	36.0
23	34.705331792912254	36.0	36.0	36.0	32.0	36.0
24	34.67339034005561	36.0	36.0	36.0	32.0	36.0
25	34.66250258535862	36.0	36.0	36.0	32.0	36.0
26	34.59007807260732	36.0	36.0	36.0	32.0	36.0
27	34.57829719402412	36.0	36.0	36.0	32.0	36.0
28	34.53464902842745	36.0	36.0	36.0	32.0	36.0
29	34.51139124675916	36.0	36.0	36.0	32.0	36.0
30	34.46656791815433	36.0	36.0	36.0	32.0	36.0
31	34.47765101105802	36.0	36.0	36.0	32.0	36.0
32	34.43812270582066	36.0	36.0	36.0	32.0	36.0
33	34.40055613936493	36.0	36.0	36.0	32.0	36.0
34	34.380135462345685	36.0	36.0	36.0	32.0	36.0
35	34.337484879569296	36.0	36.0	36.0	32.0	36.0
36	34.3023020659888	36.0	36.0	36.0	32.0	36.0
37	34.296714557605966	36.0	36.0	36.0	32.0	36.0
38	33.83601723154576	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	15.0
21	21.0
22	75.0
23	248.0
24	667.0
25	1720.0
26	3547.0
27	6866.0
28	11688.0
29	18637.0
30	28294.0
31	40426.0
32	57895.0
33	92178.0
34	221718.0
35	473317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.20862048972505	19.29576037549975	12.424064828656824	26.071554306118376
2	15.37123660575318	21.429332486519577	37.258203682386345	25.941227225340903
3	17.464593644300617	25.360226634103334	29.632597037126796	27.542582684469252
4	11.522065452084014	16.630332253572767	37.18039383230737	34.667208462035845
5	13.52013395827697	37.7608995177126	33.43880214726009	15.28016437675034
6	32.93729428097185	37.19055314197133	16.737576947142678	13.134575629914144
7	29.001934583500464	31.598580191202032	21.309458149485224	18.090027075812273
8	27.651942098989963	33.238133938653846	19.814079133887468	19.295844828468727
9	27.53207763189096	13.948168104236242	19.194139270668046	39.32561499320475
10	15.872667673659205	26.513674833334377	31.699790871466387	25.913866621540034
11	37.034278443670495	21.376653385954757	22.80727228662938	18.78179588374537
12	24.43552826615384	24.720806509065497	28.754605347687427	22.089059877093234
13	29.008202132638054	19.46157574542051	26.060051477470246	25.470170644471185
14	23.349287694528652	20.686942842160462	25.48411492989761	30.47965453341328
15	24.98751715737992	27.69404813885517	22.922990784632837	24.39544391913207
16	25.325232891193483	25.989800629678456	24.444121782403684	24.240844696724377
17	23.23605421000842	26.254812945386778	25.913858984617377	24.595273859987422
18	24.54826733966076	24.565711981648654	27.54310497913955	23.342915699551035
19	25.14232529765573	25.22254975901324	26.22420647770742	23.41091846562361
20	25.08811111087898	24.520376804266938	26.01006566288595	24.381446421968132
21	26.93097562555233	24.306758284115766	24.901547454649155	23.860718635682755
22	25.133159165288678	24.63697871020241	25.888815883624268	24.34104624088464
23	23.670782013368697	24.27204952204665	26.45389011512455	25.6032783494601
24	24.15351702785084	25.828724953359085	25.930885790869034	24.086872227921038
25	24.50193876186656	24.577880565583634	26.29330876454071	24.626871908009093
26	23.585155967634446	25.763960414242348	27.032300791589805	23.618582826533405
27	24.781132189785367	25.062335934015312	25.818201570437253	24.338330305762064
28	23.50482704734288	24.933198511669108	27.015691821074384	24.546282619913633
29	23.70705954368159	24.931213791921984	26.795805764879653	24.565920899516772
30	23.783836860215143	25.314891456721618	26.982996174713836	23.918275508349403
31	24.37611901633111	24.93413864207564	25.71664051711351	24.973101824479745
32	23.69494230733072	25.31583158712815	25.73606987884853	25.253156226692603
33	23.598004416523732	24.866553711739304	26.469371595944484	25.06607027579248
34	24.427199435086084	25.045909701519044	26.841767695865727	23.68512316752915
35	25.512423301027663	24.965685240161537	26.221699463289998	23.300191995520798
36	23.828858660794683	25.686660803038503	25.916574916902917	24.5679056192639
37	25.08852894661522	25.520988933620526	25.53373292357575	23.856749196188503
38	24.025763751496374	25.179617137114885	26.30829591962512	24.486323191763624
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	366.0
1	294.5
2	223.0
3	223.0
4	695.0
5	1167.0
6	1167.0
7	1344.0
8	1521.0
9	1484.0
10	1447.0
11	1447.0
12	1770.5
13	2094.0
14	2751.5
15	3409.0
16	3409.0
17	5073.0
18	6737.0
19	6737.0
20	7802.5
21	8868.0
22	9536.5
23	10205.0
24	10205.0
25	11632.0
26	13059.0
27	13059.0
28	16507.0
29	19955.0
30	23935.5
31	27916.0
32	27916.0
33	34630.5
34	41345.0
35	41345.0
36	43547.5
37	45750.0
38	51864.0
39	57978.0
40	57978.0
41	58786.5
42	59595.0
43	66688.0
44	73781.0
45	73781.0
46	74641.0
47	75501.0
48	75501.0
49	79445.0
50	83389.0
51	82382.0
52	81375.0
53	81375.0
54	77050.0
55	72725.0
56	72725.0
57	71092.0
58	69459.0
59	61985.5
60	54512.0
61	54512.0
62	53238.0
63	51964.0
64	43232.0
65	34500.0
66	34500.0
67	28902.5
68	23305.0
69	23305.0
70	18749.0
71	14193.0
72	10990.0
73	7787.0
74	7787.0
75	5785.5
76	3784.0
77	3784.0
78	3905.0
79	4026.0
80	3095.0
81	2164.0
82	2164.0
83	2148.0
84	2132.0
85	2132.0
86	1364.0
87	596.0
88	484.5
89	373.0
90	373.0
91	221.5
92	70.0
93	45.0
94	20.0
95	20.0
96	15.0
97	10.0
98	10.0
99	11.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008670091526918023
2	0.0
3	0.0
4	3.1337680217775985E-4
5	1.0445893405925328E-4
6	1.0445893405925328E-4
7	2.0891786811850656E-4
8	0.0026114733514813323
9	0.002820391219599839
10	8.356714724740262E-4
11	0.0037605216261331178
12	3.1337680217775985E-4
13	2.0891786811850656E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.0445893405925328E-4
23	3.1337680217775985E-4
24	0.0
25	2.0891786811850656E-4
26	0.0
27	1.0445893405925328E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	957314.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.2339876420043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.57730063608317	36.32941946550726
2	12.602094419566995	11.652901553152637
3	3.6339271484991214	5.040328286269569
4	1.5529486977371654	2.8719604359938713
5	0.8186200451185954	1.892403452475507
6	0.5199820456197501	1.4424506082748587
7	0.3860518322423229	1.249410094874527
8	0.2646945900806896	0.9790309125356795
9	0.19425052243631516	0.8082878628396126
>10	1.137536260319817	9.9844581319528
>50	0.13668987417712203	4.474830613055468
>100	0.15504933870828658	14.939403275606852
>500	0.015867622377710475	4.8516615273077015
>1k	0.004986967032994722	3.48345378015366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4372	0.45669445970705536	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3229	0.33729789807732885	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1886	0.1970095496357517	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1537	0.1605533816490723	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1509	0.15762853149541323	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1427	0.14906289890255442	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1394	0.14561575407859909	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1369	0.14300428072711774	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1337	0.13966159483722165	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1305	0.13631890894732554	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1283	0.13402081239802197	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1261	0.1317227158487184	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1207	0.1260819334095187	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1205	0.1258730155414002	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1179	0.12315708325585963	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1163	0.12148574031091157	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1157	0.12085898670655605	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1142	0.11929210269566726	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1142	0.11929210269566726	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1097	0.11459145066300086	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1024	0.10696594847667536	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1013	0.10581690020202358	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	961	0.10038503563094242	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0891786811850658E-4
2	0.0	0.0	0.0	0.0	2.0891786811850658E-4
3	0.0	0.0	0.0	0.0	2.0891786811850658E-4
4	0.0	0.0	0.0	0.0	2.0891786811850658E-4
5	0.0	0.0	0.0	0.0	2.0891786811850658E-4
6	0.0	0.0	0.0	0.0	2.0891786811850658E-4
7	0.0	0.0	0.0	0.0	2.0891786811850658E-4
8	0.0	0.0	0.0	0.0	2.0891786811850658E-4
9	0.0	0.0	0.0	0.0	2.0891786811850658E-4
10	0.0	0.0	0.0	0.0	2.0891786811850658E-4
11	0.0	0.0	0.0	0.0	2.0891786811850658E-4
12	0.0	0.0	0.0	0.0	2.0891786811850658E-4
13	0.0	0.0	0.0	0.0	2.0891786811850658E-4
14	0.0	0.0	0.0	0.0	2.0891786811850658E-4
15	0.0	0.0	0.0	1.0445893405925329E-4	2.0891786811850658E-4
16	0.0	0.0	0.0	1.0445893405925329E-4	2.0891786811850658E-4
17	0.0	0.0	0.0	1.0445893405925329E-4	2.0891786811850658E-4
18	0.0	0.0	0.0	1.0445893405925329E-4	2.0891786811850658E-4
19	0.0	0.0	0.0	1.0445893405925329E-4	2.0891786811850658E-4
20	0.0	0.0	0.0	1.0445893405925329E-4	2.0891786811850658E-4
21	0.0	0.0	0.0	3.1337680217775985E-4	2.0891786811850658E-4
22	0.0	0.0	0.0	7.31212538414773E-4	2.0891786811850658E-4
23	0.0	0.0	0.0	0.0019847197471258124	3.1337680217775985E-4
24	0.0	0.0	0.0	0.003238226955836852	3.1337680217775985E-4
25	0.0	0.0	0.0	0.003969439494251625	3.1337680217775985E-4
26	0.0	0.0	0.0	0.005327405637021917	3.1337680217775985E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAA	20	0.003753475	31.99953	22
ACCGTCG	240	0.0	22.000826	8
ACCTCGC	40	0.004481967	20.000751	8
ATACCGT	275	0.0	19.78256	6
CCGTCGT	260	0.0	19.693047	9
TACCGTC	280	0.0	19.4293	7
CGTCGTA	265	0.0	19.32047	10
ATAACAC	50	7.1929186E-4	19.200722	3
TCAGATA	295	0.0	18.982773	2
TCTAGCG	315	0.0	18.793375	28
TCGAACT	60	1.13805836E-4	18.666391	19
AACCGCG	245	0.0	18.2864	7
CTAGCGG	330	0.0	17.93913	29
CGTGATC	45	0.008849974	17.777515	13
GTCGTAG	290	0.0	17.654913	11
ATTGTCG	55	0.0013640769	17.4552	6
CGAACTC	65	2.100177E-4	17.230516	20
TTAGGAC	215	0.0	17.116922	3
GAACCGC	265	0.0	16.906294	6
AGAACCG	275	0.0	16.87336	5
>>END_MODULE