FastQCFastQC Report
Thu 2 Feb 2017
SRR4062769_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062769_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637702
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT58960.36001665748713746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT39660.24216859965976717No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC34200.20882920091689455No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT31380.19160995101672954No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA29820.18208440851876592No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG29040.17732163726978414No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG28590.17457388462614076No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28350.173108416549531No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG27530.16810140062111423No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC26710.16309438469269744No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG25820.1576599405752695No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT24800.15143170124967792No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA24650.15051578370179677No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG23870.14575301245281497No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23860.14569195128295623No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA22910.13989114014637583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22770.13903628376835345No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT22730.1387920390889185No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG22370.13659383697400382No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG21180.12932755776081362No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC20900.1276178450047689No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC20640.1260302545884416No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20030.12230552322705841No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT19860.1212674833394598No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT19710.12035156579157869No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT19710.12035156579157869No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19320.1179701801670878No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA18940.11564985571245563No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG18730.11436757114542206No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT18260.11149769616206123No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG18050.11021541159502767No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG17730.10826145415954797No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC16920.10331549940098993No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT16830.10276594887226125No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16730.10215533717367385No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT16570.10117835845593398No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC16440.10038456324777034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT6050.020.09800329
TTCTACG400.0044731220.0086122
TAATACC1154.5474735E-1119.474754
CATCGTT6250.019.45427328
ACCGTAC507.2005426E-419.1988838
TCTAGCG6650.018.76525128
GCATCGT6350.018.64344627
GTCCTAA1900.018.542791
AAGACGG7000.017.8253575
TCGTTTA6850.017.75079230
CTAGCGG6950.017.72558629
TATAAGA1101.0584699E-817.462062
TAGCGGC7450.016.75070430
CGGCATC7200.016.66620625
CAAGACG7500.016.6374
GGCATCG7250.016.55126626
CGTTTAT7500.016.2123931
TAATACT2500.015.9971154
CGCAAGA7350.015.8982
GTCCTAT6750.015.6583571
Produced by FastQC (version 0.11.5)