##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062769_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1637702
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244954210228723	32.0	32.0	32.0	32.0	32.0
2	30.913919015791638	32.0	32.0	32.0	32.0	32.0
3	30.968402676433197	32.0	32.0	32.0	32.0	32.0
4	31.01410879390756	32.0	32.0	32.0	32.0	32.0
5	30.958513819974574	32.0	32.0	32.0	32.0	32.0
6	34.600278316812215	36.0	36.0	36.0	32.0	36.0
7	34.516129918629886	36.0	36.0	36.0	32.0	36.0
8	34.50880135702344	36.0	36.0	36.0	32.0	36.0
9	34.615351266591844	36.0	36.0	36.0	32.0	36.0
10	34.37593591508101	36.0	36.0	36.0	32.0	36.0
11	34.597769313342724	36.0	36.0	36.0	32.0	36.0
12	34.44109917433086	36.0	36.0	36.0	32.0	36.0
13	34.52872989103024	36.0	36.0	36.0	32.0	36.0
14	34.42609033877958	36.0	36.0	36.0	32.0	36.0
15	34.38373709014216	36.0	36.0	36.0	32.0	36.0
16	34.390197361913216	36.0	36.0	36.0	32.0	36.0
17	34.307865533534184	36.0	36.0	36.0	32.0	36.0
18	34.3305534218069	36.0	36.0	36.0	32.0	36.0
19	34.31791253842274	36.0	36.0	36.0	32.0	36.0
20	34.30252451300664	36.0	36.0	36.0	32.0	36.0
21	34.27213070509775	36.0	36.0	36.0	32.0	36.0
22	34.25253739691348	36.0	36.0	36.0	32.0	36.0
23	34.20866799942847	36.0	36.0	36.0	32.0	36.0
24	34.18425208004875	36.0	36.0	36.0	32.0	36.0
25	34.15804035166349	36.0	36.0	36.0	32.0	36.0
26	34.10306209554608	36.0	36.0	36.0	32.0	36.0
27	34.09975563319823	36.0	36.0	36.0	32.0	36.0
28	34.09438102902726	36.0	36.0	36.0	32.0	36.0
29	34.061586295919525	36.0	36.0	36.0	32.0	36.0
30	34.024000703424676	36.0	36.0	36.0	32.0	36.0
31	34.04559071186333	36.0	36.0	36.0	32.0	36.0
32	33.99711974461776	36.0	36.0	36.0	32.0	36.0
33	33.94354955907729	36.0	36.0	36.0	32.0	36.0
34	33.94591873246781	36.0	36.0	36.0	32.0	36.0
35	33.90431287254946	36.0	36.0	36.0	32.0	36.0
36	33.867871566377765	36.0	36.0	36.0	32.0	36.0
37	33.854337358078574	36.0	36.0	36.0	32.0	36.0
38	33.230421041190645	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	0.0
6	0.0
7	1.0
8	1.0
9	8.0
10	17.0
11	19.0
12	4.0
13	9.0
14	182.0
15	461.0
16	602.0
17	712.0
18	935.0
19	1248.0
20	1615.0
21	2210.0
22	3313.0
23	4957.0
24	7111.0
25	10641.0
26	15290.0
27	21896.0
28	30466.0
29	41578.0
30	55592.0
31	75451.0
32	103651.0
33	155049.0
34	349473.0
35	755209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.26782730750115	16.825909376847378	11.401468305616401	25.50479501003507
2	17.045553129433156	19.246536258854093	36.90064967675008	26.807260934962677
3	18.893334652406025	23.55234825473248	28.01460018051754	29.539716912343955
4	12.434174209968393	14.665839468117847	35.306682233631484	37.593304088282274
5	15.159519740220565	36.03432136713934	32.71527028504052	16.090888607599574
6	35.29989692836766	34.82233576927586	16.257046484817693	13.620720817538787
7	31.264864587613978	29.957177775430317	20.307675210554308	18.4702824264014
8	29.400823960935433	31.61588301635404	19.012223857986115	19.97106916472441
9	27.820469842712054	13.68910121957847	18.31065209254215	40.17977684516733
10	16.606572041802657	25.414988749431366	30.107620771877546	27.870818436888435
11	38.703082676097964	20.664322683230058	21.344924612314582	19.287670028357397
12	25.588556773425335	23.323603944676822	27.431441394681404	23.65639788721644
13	29.678056061251002	18.325100048306552	25.339138262393135	26.65770562804931
14	24.455098611090754	19.42164607485356	24.12093965108724	32.00231566296844
15	25.770834707533545	26.401317766393294	22.13928160477269	25.688565921300473
16	26.539408566166234	25.16805983711875	23.10529632759943	25.187235269115586
17	24.52597531883603	25.39676626182848	24.588820309742136	25.48843810959336
18	26.14883605049786	23.47598396203257	25.929126155492078	24.446053831977498
19	26.126962337291186	24.390200709824448	25.06369123369248	24.419145719191885
20	26.260027613530262	23.138208711146717	24.69597332198341	25.905790353339608
21	28.077413240465777	23.273740062445004	23.767633263536112	24.881213433553107
22	26.48714236251265	23.271007891489123	24.73309814989982	25.508751596098406
23	24.36117747065117	23.021549344628298	25.62044134386618	26.99683184085435
24	25.17475214692005	24.58310482930291	24.73626868898332	25.50587433479372
25	25.385682258035953	23.759590527310294	24.720124244010254	26.134602970643506
26	24.841744224465778	25.09513668474228	25.4791645927246	24.58395449806734
27	26.026083913820614	23.938405958617725	24.445684771437524	25.58982535612414
28	24.728582620860806	23.76565317563982	25.693527201581333	25.812237001918042
29	24.461504075665456	24.31830006766313	25.79101130731519	25.429184549356222
30	24.481893839148487	24.57458780799525	26.014702802252256	24.928815550604007
31	25.483769915492545	24.44634152150058	24.080075378280625	25.989813184726252
32	25.015693774761967	24.213235042672412	24.06557921631273	26.705491966252886
33	24.4295986443369	23.86425049234386	24.955925682793154	26.750225180526083
34	25.69851609794645	24.15171354992681	25.038305179764116	25.111465172362625
35	26.66085272027956	23.775926109138524	25.197167288978783	24.366053881603133
36	24.694878229160878	25.140262882596897	24.401008634709466	25.763850253532755
37	26.290019315405367	24.657278268245364	24.489650105981305	24.56305231036797
38	24.91191431415294	24.324167536837223	24.990260196261623	25.773657952748213
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	110.0
1	156.0
2	202.0
3	202.0
4	602.5
5	1003.0
6	1003.0
7	1301.0
8	1599.0
9	1611.0
10	1623.0
11	1623.0
12	2063.0
13	2503.0
14	3298.0
15	4093.0
16	4093.0
17	6093.5
18	8094.0
19	8094.0
20	9432.5
21	10771.0
22	10509.5
23	10248.0
24	10248.0
25	11206.5
26	12165.0
27	12165.0
28	15727.5
29	19290.0
30	24595.5
31	29901.0
32	29901.0
33	39508.5
34	49116.0
35	49116.0
36	54445.5
37	59775.0
38	70424.0
39	81073.0
40	81073.0
41	86664.0
42	92255.0
43	108221.0
44	124187.0
45	124187.0
46	128544.5
47	132902.0
48	132902.0
49	141434.0
50	149966.0
51	152838.5
52	155711.0
53	155711.0
54	148366.0
55	141021.0
56	141021.0
57	139126.5
58	137232.0
59	124089.5
60	110947.0
61	110947.0
62	108635.5
63	106324.0
64	88078.0
65	69832.0
66	69832.0
67	59077.5
68	48323.0
69	48323.0
70	38910.0
71	29497.0
72	23064.0
73	16631.0
74	16631.0
75	12406.5
76	8182.0
77	8182.0
78	8300.5
79	8419.0
80	6594.5
81	4770.0
82	4770.0
83	4883.5
84	4997.0
85	4997.0
86	3329.5
87	1662.0
88	1432.0
89	1202.0
90	1202.0
91	874.5
92	547.0
93	483.5
94	420.0
95	420.0
96	364.0
97	308.0
98	308.0
99	554.5
100	801.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11839760835609897
2	0.05422231883456209
3	0.011479499933443325
4	0.00280881381350209
5	3.053058492937055E-4
6	3.6636701915244654E-4
7	6.106116985874109E-5
8	1.8318350957622327E-4
9	3.053058492937055E-4
10	4.274281890111876E-4
11	0.002076079775197197
12	0.003175180832654537
13	0.014837864275674085
14	0.009647664837681091
15	0.02356961156547406
16	0.011601622273160807
17	0.020760797751971972
18	0.005373382947569216
19	0.006960973363896484
20	0.006045055816015368
21	0.00616717815573285
22	0.007022034533755226
23	0.009647664837681091
24	0.014654680766097862
25	0.017036066390588765
26	0.01960063552465589
27	0.008609624950082493
28	0.005800811136580404
29	0.010868888234855914
30	0.00335836434223076
31	0.005800811136580404
32	0.00671672868446152
33	0.0077547685720601185
34	0.011906928122454512
35	0.015875904163272683
36	0.014715741935956603
37	0.009098114308952422
38	0.00561762762700418
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1637702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.36415494638828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.74255536295219	28.2408506555709
2	14.519589292260507	11.431027253169255
3	5.56037122267248	6.566379431061539
4	2.646558416270847	4.1671814229101445
5	1.4512938944825298	2.8564478867578793
6	0.8585951621374435	2.0278723799158613
7	0.5970011953112166	1.6450313288786822
8	0.4195792168507198	1.3213105043516786
9	0.29657885632396375	1.0507118448743225
>10	1.6251008328763812	11.503509293022542
>50	0.12458826222795341	3.4213635741220383
>100	0.12191092116801067	10.489765361872326
>500	0.022420345420952777	5.989381473158696
>1k	0.013701322201693364	8.927809140355295
>5k	1.5569684320106093E-4	0.36135844997895783
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5896	0.36001665748713746	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3966	0.24216859965976717	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3420	0.20882920091689455	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3138	0.19160995101672954	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2982	0.18208440851876592	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2904	0.17732163726978414	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2859	0.17457388462614076	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2835	0.173108416549531	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2753	0.16810140062111423	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2671	0.16309438469269744	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2582	0.1576599405752695	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2480	0.15143170124967792	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2465	0.15051578370179677	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2387	0.14575301245281497	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2386	0.14569195128295623	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2291	0.13989114014637583	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2277	0.13903628376835345	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2273	0.1387920390889185	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2237	0.13659383697400382	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2118	0.12932755776081362	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2090	0.1276178450047689	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2064	0.1260302545884416	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2003	0.12230552322705841	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1986	0.1212674833394598	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1971	0.12035156579157869	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1971	0.12035156579157869	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1932	0.1179701801670878	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1894	0.11564985571245563	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1873	0.11436757114542206	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1826	0.11149769616206123	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1805	0.11021541159502767	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1773	0.10826145415954797	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1692	0.10331549940098993	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1683	0.10276594887226125	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1673	0.10215533717367385	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1657	0.10117835845593398	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1644	0.10038456324777034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.2212233971748218E-4
7	0.0	0.0	0.0	0.0	1.2212233971748218E-4
8	0.0	0.0	0.0	0.0	1.8318350957622327E-4
9	0.0	0.0	0.0	0.0	1.8318350957622327E-4
10	0.0	0.0	0.0	0.0	1.8318350957622327E-4
11	6.106116985874109E-5	0.0	0.0	0.0	1.8318350957622327E-4
12	6.106116985874109E-5	0.0	0.0	0.0	1.8318350957622327E-4
13	6.106116985874109E-5	0.0	0.0	0.0	1.8318350957622327E-4
14	6.106116985874109E-5	0.0	0.0	0.0	3.053058492937054E-4
15	6.106116985874109E-5	0.0	0.0	0.0	7.937952081636341E-4
16	6.106116985874109E-5	0.0	0.0	1.2212233971748218E-4	7.937952081636341E-4
17	6.106116985874109E-5	0.0	0.0	1.8318350957622327E-4	7.937952081636341E-4
18	6.106116985874109E-5	0.0	0.0	1.8318350957622327E-4	9.769787177398574E-4
19	6.106116985874109E-5	0.0	0.0	2.4424467943496436E-4	9.769787177398574E-4
20	6.106116985874109E-5	0.0	0.0	3.053058492937054E-4	9.769787177398574E-4
21	6.106116985874109E-5	0.0	0.0	4.274281890111876E-4	0.0010991010574573396
22	6.106116985874109E-5	0.0	0.0	6.71672868446152E-4	0.0010991010574573396
23	6.106116985874109E-5	0.0	0.0	0.0010991010574573396	0.0011601622273160806
24	6.106116985874109E-5	0.0	0.0	0.001953957435479715	0.0012212233971748217
25	6.106116985874109E-5	0.0	0.0	0.002137140945055938	0.0012212233971748217
26	6.106116985874109E-5	0.0	0.0	0.00280881381350209	0.0012212233971748217
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	605	0.0	20.098003	29
TTCTACG	40	0.00447312	20.008612	2
TAATACC	115	4.5474735E-11	19.47475	4
CATCGTT	625	0.0	19.454273	28
ACCGTAC	50	7.2005426E-4	19.198883	8
TCTAGCG	665	0.0	18.765251	28
GCATCGT	635	0.0	18.643446	27
GTCCTAA	190	0.0	18.54279	1
AAGACGG	700	0.0	17.825357	5
TCGTTTA	685	0.0	17.750792	30
CTAGCGG	695	0.0	17.725586	29
TATAAGA	110	1.0584699E-8	17.46206	2
TAGCGGC	745	0.0	16.750704	30
CGGCATC	720	0.0	16.666206	25
CAAGACG	750	0.0	16.637	4
GGCATCG	725	0.0	16.551266	26
CGTTTAT	750	0.0	16.21239	31
TAATACT	250	0.0	15.997115	4
CGCAAGA	735	0.0	15.898	2
GTCCTAT	675	0.0	15.658357	1
>>END_MODULE