FastQCFastQC Report
Thu 2 Feb 2017
SRR4062769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637702
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT61910.3780297025954661No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT45490.2777672616874132No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC35780.21847686575457562No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT32310.19728863981359246No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA31110.18996129943054352No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG29900.18257289787763586No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC29890.1825118367077771No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT27950.17066596975518136No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC27800.16975005220730022No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG27450.1676129112622443No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG26860.16401030224057858No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA26550.16211740597495758No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA25390.15503431027134362No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG24680.15069896721137302No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24400.14898925445532826No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT23590.14404329969677024No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC23570.14392117735705273No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC23250.14196721992157305No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG23060.14080705769425694No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA22890.13976901780665835No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG21450.13097620934699963No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA21330.13024347530869476No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT21130.12902225191151992No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT20680.12627449926787657No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT20520.1252975205501367No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG19370.1182754860163815No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT19210.11729850729864162No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18800.11479499933443324No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC18600.11357377593725843No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT18600.11357377593725843No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG18470.11277998072909479No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC18460.11271891955923605No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG17980.10978798340601649No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC17750.10838357649926543No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA17500.1068570472527969No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT17040.10404823343929481No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC16980.10368186642014238No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT16860.10294913238183746No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG16500.10075093026692279No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT16460.10050668558748783No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT16390.10007925739847666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA703.0875526E-822.85620532
TCTAGCG6200.019.09599128
CTAGCGG6550.018.07559429
GGTTTAG550.001365077617.4543611
TAGCGGC7000.017.14215530
CATCGTT5000.016.95930528
TTAGGCA854.981237E-616.9430684
AAGACGG8100.016.5949525
TCGTTTA5300.016.30121830
CAAGACG8000.016.0017874
AGGCCCG7600.016.0003210
ATCGTTT5500.015.99934429
CGTTTAT5400.015.99934331
CGAAAAA4150.015.8065824
CGGCATC5400.015.70305925
TTTTCGG7200.015.55491729
TATACTG1753.6379788E-1215.5450685
GATATAC1753.6379788E-1215.5426941
CTATACT1451.2205419E-915.454
AACCGCG4050.015.4095977
Produced by FastQC (version 0.11.5)