##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062769_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1637702
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.166082107733885	32.0	32.0	32.0	32.0	32.0
2	31.280101630211114	32.0	32.0	32.0	32.0	32.0
3	31.36592921056456	32.0	32.0	32.0	32.0	32.0
4	31.472400961835547	32.0	32.0	32.0	32.0	32.0
5	31.375808907847702	32.0	32.0	32.0	32.0	32.0
6	34.909493302200275	36.0	36.0	36.0	36.0	36.0
7	34.93728529366148	36.0	36.0	36.0	36.0	36.0
8	34.87949211761358	36.0	36.0	36.0	32.0	36.0
9	34.98289859815766	36.0	36.0	36.0	36.0	36.0
10	34.82329935482768	36.0	36.0	36.0	32.0	36.0
11	35.00763753112593	36.0	36.0	36.0	36.0	36.0
12	34.88716140054784	36.0	36.0	36.0	32.0	36.0
13	34.95542717783822	36.0	36.0	36.0	36.0	36.0
14	34.89514575911857	36.0	36.0	36.0	32.0	36.0
15	34.86286943534294	36.0	36.0	36.0	32.0	36.0
16	34.87115971037466	36.0	36.0	36.0	32.0	36.0
17	34.82753638940418	36.0	36.0	36.0	32.0	36.0
18	34.82512019891287	36.0	36.0	36.0	32.0	36.0
19	34.823410486156824	36.0	36.0	36.0	32.0	36.0
20	34.80112499099348	36.0	36.0	36.0	32.0	36.0
21	34.790249386029934	36.0	36.0	36.0	32.0	36.0
22	34.77639399597729	36.0	36.0	36.0	32.0	36.0
23	34.71331292261962	36.0	36.0	36.0	32.0	36.0
24	34.67566749017831	36.0	36.0	36.0	32.0	36.0
25	34.662839149002686	36.0	36.0	36.0	32.0	36.0
26	34.586385068834254	36.0	36.0	36.0	32.0	36.0
27	34.575241405335035	36.0	36.0	36.0	32.0	36.0
28	34.54452824750779	36.0	36.0	36.0	32.0	36.0
29	34.5006045055816	36.0	36.0	36.0	32.0	36.0
30	34.47173844814258	36.0	36.0	36.0	32.0	36.0
31	34.47748613606139	36.0	36.0	36.0	32.0	36.0
32	34.44303176035689	36.0	36.0	36.0	32.0	36.0
33	34.39873249223607	36.0	36.0	36.0	32.0	36.0
34	34.381167636114505	36.0	36.0	36.0	32.0	36.0
35	34.34240722671157	36.0	36.0	36.0	32.0	36.0
36	34.307027163672025	36.0	36.0	36.0	32.0	36.0
37	34.29962777110854	36.0	36.0	36.0	32.0	36.0
38	33.830488696966846	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	12.0
21	31.0
22	112.0
23	448.0
24	1128.0
25	2883.0
26	5964.0
27	11540.0
28	20001.0
29	31275.0
30	47448.0
31	67904.0
32	97974.0
33	157551.0
34	379005.0
35	814423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5389819164651	18.121945544823507	12.106741281425432	26.232331257285963
2	16.084916547699155	20.171801707514554	36.095822072635926	27.647459672150365
3	17.89434219412323	24.197076146942482	28.367248742445206	29.541332916489083
4	11.95354699095926	15.432393518218865	35.328857945726256	37.28520154509562
5	14.572858798487148	36.60440055638938	32.3894701233802	16.43327052174327
6	35.369197880685036	35.239076289354756	15.865797072600033	13.525928757360175
7	30.97471632420405	30.21096087982087	20.231764483906364	18.582558312068716
8	29.236270510197166	31.832473265224	18.823553836844134	20.1077023877347
9	27.768112685276158	13.462744477819378	18.071253273799893	40.697889563104575
10	16.43698185053359	25.72512684109645	30.03024402218017	27.807647286189784
11	38.862709441515754	20.571264920198654	21.194238743940172	19.371786894345423
12	25.391631317316165	23.419049020547707	27.46203366792839	23.727285994207733
13	29.733076550133177	18.344346759903228	25.283416112128123	26.639160577835476
14	24.29167473183444	19.389558899945715	24.08479936203251	32.23396700618733
15	25.780636526059077	26.600443792582535	21.94092698183186	25.67799269952653
16	26.606993584298966	25.085104057456153	22.99607803866518	25.3118243195797
17	24.512273905753307	25.492549926665536	24.475942509687354	25.519233657893803
18	26.148102646268978	23.327809332833446	25.994655926413962	24.52943209448361
19	26.156223781860195	24.35919355291744	25.003877384286028	24.480705280936334
20	26.390942918797194	22.97884474709074	24.671643559084618	25.958568775027445
21	28.136332578413274	23.30706276664666	23.753847619315124	24.80275703562494
22	26.403399397448375	23.34368523699672	24.742413454950903	25.510501910604006
23	24.321670635647553	22.833547047688832	25.718935091897173	27.125847224766442
24	25.16916997109364	24.51886851209805	24.733620646491243	25.578340870317067
25	25.505327285017227	23.598568969549387	24.748656806095862	26.14744693933752
26	25.062206104777367	24.828463803832324	25.399325029416236	24.710005061974073
27	26.0791353727789	23.819075532759356	24.434511815350795	25.66727727911095
28	24.782897010567247	23.550194113458982	25.888897980218623	25.77801089575515
29	24.58487563671535	24.14438035735439	25.87180085265818	25.398943153272086
30	24.653080963447564	24.34087520195982	26.005707998158396	25.000335836434225
31	25.51764606747748	24.22259971594344	24.206723811780165	26.05303040479892
32	25.24055047865851	24.092173057125166	24.151829820077158	26.515446644139168
33	24.571381118176568	23.601851863159474	25.061824434481977	26.764942584181984
34	25.692281013273476	23.846279726104015	25.29287990122745	25.168559359395054
35	26.6972868079785	23.621086131664978	25.22021710909555	24.461409951260972
36	24.949166576092598	24.810069231154387	24.515265903076383	25.725498289676636
37	26.39784283099123	24.423613087118415	24.533095764675135	24.64544831721522
38	25.094247915676966	23.995879592257932	25.049367955830792	25.86050453623431
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	60.0
1	84.5
2	109.0
3	109.0
4	479.0
5	849.0
6	849.0
7	1142.0
8	1435.0
9	1537.5
10	1640.0
11	1640.0
12	2110.0
13	2580.0
14	3375.5
15	4171.0
16	4171.0
17	6335.0
18	8499.0
19	8499.0
20	9552.0
21	10605.0
22	10504.5
23	10404.0
24	10404.0
25	11127.0
26	11850.0
27	11850.0
28	15402.0
29	18954.0
30	23914.0
31	28874.0
32	28874.0
33	39465.0
34	50056.0
35	50056.0
36	54818.5
37	59581.0
38	69811.0
39	80041.0
40	80041.0
41	85834.0
42	91627.0
43	107715.5
44	123804.0
45	123804.0
46	128199.0
47	132594.0
48	132594.0
49	142069.5
50	151545.0
51	153847.0
52	156149.0
53	156149.0
54	148738.0
55	141327.0
56	141327.0
57	139453.0
58	137579.0
59	124880.0
60	112181.0
61	112181.0
62	109560.5
63	106940.0
64	88913.5
65	70887.0
66	70887.0
67	60028.5
68	49170.0
69	49170.0
70	39584.0
71	29998.0
72	23428.0
73	16858.0
74	16858.0
75	12288.0
76	7718.0
77	7718.0
78	8049.5
79	8381.0
80	6530.0
81	4679.0
82	4679.0
83	4479.0
84	4279.0
85	4279.0
86	2749.0
87	1219.0
88	993.5
89	768.0
90	768.0
91	469.0
92	170.0
93	115.5
94	61.0
95	61.0
96	45.5
97	30.0
98	30.0
99	30.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008243257930930047
2	0.0
3	0.0
4	2.4424467943496436E-4
5	0.0
6	1.8318350957622327E-4
7	3.053058492937055E-4
8	0.0025035079642083844
9	0.004823832418840546
10	0.001404406906751045
11	0.004457465399688099
12	6.106116985874109E-5
13	2.4424467943496436E-4
14	6.106116985874109E-5
15	0.0
16	6.106116985874109E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	6.106116985874109E-5
22	0.0
23	1.2212233971748218E-4
24	0.0
25	6.106116985874109E-5
26	6.106116985874109E-5
27	1.2212233971748218E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1637702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.97323685737122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.2101476144851	25.58923180683983
2	15.730816267182274	11.632383916126365
3	5.9965816135944205	6.651390970019514
4	2.9189495818952804	4.316920570645555
5	1.6347468196534538	3.0220940682440727
6	0.9701823989462519	2.1522470178655433
7	0.6403076717657931	1.6571973046851998
8	0.46132938088447745	1.3645472374964964
9	0.34283655862218376	1.1408199556773628
>10	1.7872954164503931	11.923167329596124
>50	0.13444827999001563	3.490172309561797
>100	0.13047385320591687	10.506200418556114
>500	0.02659336084100039	6.664418540770795
>1k	0.015124973978318973	9.508755109076123
>5k	1.6620850525625244E-4	0.3804534448391117
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6191	0.3780297025954661	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4549	0.2777672616874132	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3578	0.21847686575457562	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3231	0.19728863981359246	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3111	0.18996129943054352	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2990	0.18257289787763586	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2989	0.1825118367077771	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2795	0.17066596975518136	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2780	0.16975005220730022	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2745	0.1676129112622443	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2686	0.16401030224057858	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2655	0.16211740597495758	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2539	0.15503431027134362	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2468	0.15069896721137302	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2440	0.14898925445532826	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2359	0.14404329969677024	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2357	0.14392117735705273	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2325	0.14196721992157305	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2306	0.14080705769425694	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2289	0.13976901780665835	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	2145	0.13097620934699963	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2133	0.13024347530869476	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	2113	0.12902225191151992	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2068	0.12627449926787657	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2052	0.1252975205501367	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1937	0.1182754860163815	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1921	0.11729850729864162	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1880	0.11479499933443324	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1860	0.11357377593725843	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1860	0.11357377593725843	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1847	0.11277998072909479	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1846	0.11271891955923605	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1798	0.10978798340601649	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1775	0.10838357649926543	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	1750	0.1068570472527969	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	1704	0.10404823343929481	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1698	0.10368186642014238	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1686	0.10294913238183746	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1650	0.10075093026692279	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1646	0.10050668558748783	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1639	0.10007925739847666	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	6.106116985874109E-5
5	0.0	0.0	0.0	0.0	6.106116985874109E-5
6	0.0	0.0	0.0	0.0	2.4424467943496436E-4
7	0.0	0.0	0.0	6.106116985874109E-5	2.4424467943496436E-4
8	0.0	0.0	0.0	6.106116985874109E-5	2.4424467943496436E-4
9	0.0	0.0	0.0	1.2212233971748218E-4	3.053058492937054E-4
10	0.0	0.0	0.0	1.2212233971748218E-4	3.053058492937054E-4
11	0.0	0.0	0.0	1.2212233971748218E-4	3.053058492937054E-4
12	0.0	0.0	0.0	1.2212233971748218E-4	4.274281890111876E-4
13	0.0	0.0	0.0	1.2212233971748218E-4	4.274281890111876E-4
14	0.0	0.0	0.0	1.2212233971748218E-4	7.327340383048931E-4
15	0.0	0.0	0.0	1.2212233971748218E-4	0.0010380398875985985
16	0.0	0.0	0.0	2.4424467943496436E-4	0.0010380398875985985
17	0.0	0.0	0.0	3.053058492937054E-4	0.0010380398875985985
18	0.0	0.0	0.0	3.6636701915244654E-4	0.0010380398875985985
19	0.0	0.0	0.0	4.274281890111876E-4	0.0010380398875985985
20	0.0	0.0	0.0	4.884893588699287E-4	0.0011601622273160806
21	0.0	0.0	0.0	6.106116985874108E-4	0.0011601622273160806
22	0.0	0.0	0.0	9.159175478811164E-4	0.0011601622273160806
23	0.0	0.0	0.0	0.001343345736892304	0.0015265292464685272
24	0.0	0.0	0.0	0.002137140945055938	0.0015875904163272683
25	0.0	0.0	0.0	0.002503507964208385	0.0017097127560447504
26	0.0	0.0	0.0	0.0029919973230783134	0.0017097127560447504
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	70	3.0875526E-8	22.856205	32
TCTAGCG	620	0.0	19.095991	28
CTAGCGG	655	0.0	18.075594	29
GGTTTAG	55	0.0013650776	17.454361	1
TAGCGGC	700	0.0	17.142155	30
CATCGTT	500	0.0	16.959305	28
TTAGGCA	85	4.981237E-6	16.943068	4
AAGACGG	810	0.0	16.594952	5
TCGTTTA	530	0.0	16.301218	30
CAAGACG	800	0.0	16.001787	4
AGGCCCG	760	0.0	16.00032	10
ATCGTTT	550	0.0	15.999344	29
CGTTTAT	540	0.0	15.999343	31
CGAAAAA	415	0.0	15.80658	24
CGGCATC	540	0.0	15.703059	25
TTTTCGG	720	0.0	15.554917	29
TATACTG	175	3.6379788E-12	15.545068	5
GATATAC	175	3.6379788E-12	15.542694	1
CTATACT	145	1.2205419E-9	15.45	4
AACCGCG	405	0.0	15.409597	7
>>END_MODULE