FastQCFastQC Report
Thu 2 Feb 2017
SRR4062768_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062768_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647675
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30930.47755432894584476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21570.3330374030184892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12500.1929980314200795No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9810.1514648550584784No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8520.1315474582159262No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8300.1281506928629328No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8240.12722430231211643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCGT805.2514224E-921.99686827
TAACCTA400.004485096319.9971545
TAGCGTA507.1981904E-419.1972687
CGCAAGA802.7797068E-618.008562
TCTTTCG802.7920378E-618.00021710
CGAACCC1006.799564E-817.60021210
CATCGTT1006.8115696E-817.59749428
AATAGCG550.001365075617.452065
TCCGGAA1201.6443664E-917.3415762
AAGACGG1201.6570993E-917.3308665
ACCGTCG1505.456968E-1217.0642388
ATCGTTT1051.1886368E-716.7595229
CAAGACG1151.8397259E-816.6932774
GATATAA600.002415119216.024941
GCGACGA703.6929466E-416.00019313
CGTCGTA1501.1459633E-1016.00019310
ACGACCC600.002440164616.00019316
TCGTTTA1102.0166772E-715.99895930
ATATACC805.6179124E-515.9989593
ATACCGT1703.6379788E-1215.9977236
Produced by FastQC (version 0.11.5)