##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062768_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	647675
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.166565021036785	32.0	32.0	32.0	32.0	32.0
2	30.84837457057938	32.0	32.0	32.0	32.0	32.0
3	30.91213494422357	32.0	32.0	32.0	32.0	32.0
4	30.95911375303972	32.0	32.0	32.0	32.0	32.0
5	30.917296483575868	32.0	32.0	32.0	32.0	32.0
6	34.55111437063342	36.0	36.0	36.0	32.0	36.0
7	34.45656849500135	36.0	36.0	36.0	32.0	36.0
8	34.44417493341568	36.0	36.0	36.0	32.0	36.0
9	34.52971320492531	36.0	36.0	36.0	32.0	36.0
10	34.32285637086502	36.0	36.0	36.0	32.0	36.0
11	34.580419191724246	36.0	36.0	36.0	32.0	36.0
12	34.42554521943876	36.0	36.0	36.0	32.0	36.0
13	34.480892422897284	36.0	36.0	36.0	32.0	36.0
14	34.385242598525494	36.0	36.0	36.0	32.0	36.0
15	34.36046319527541	36.0	36.0	36.0	32.0	36.0
16	34.36389084031343	36.0	36.0	36.0	32.0	36.0
17	34.30031420079515	36.0	36.0	36.0	32.0	36.0
18	34.31608908789131	36.0	36.0	36.0	32.0	36.0
19	34.30603003049369	36.0	36.0	36.0	32.0	36.0
20	34.27208090477477	36.0	36.0	36.0	32.0	36.0
21	34.24043077160613	36.0	36.0	36.0	32.0	36.0
22	34.218964758559466	36.0	36.0	36.0	32.0	36.0
23	34.18912726290192	36.0	36.0	36.0	32.0	36.0
24	34.184127841896014	36.0	36.0	36.0	32.0	36.0
25	34.16953873470491	36.0	36.0	36.0	32.0	36.0
26	34.11816111475663	36.0	36.0	36.0	32.0	36.0
27	34.12089087891304	36.0	36.0	36.0	32.0	36.0
28	34.105707337785155	36.0	36.0	36.0	32.0	36.0
29	34.069499363106495	36.0	36.0	36.0	32.0	36.0
30	34.04573898946231	36.0	36.0	36.0	32.0	36.0
31	34.05649747172579	36.0	36.0	36.0	32.0	36.0
32	33.99711120546571	36.0	36.0	36.0	32.0	36.0
33	33.95540819083645	36.0	36.0	36.0	32.0	36.0
34	33.94971706488594	36.0	36.0	36.0	32.0	36.0
35	33.89978152622843	36.0	36.0	36.0	32.0	36.0
36	33.87003203767321	36.0	36.0	36.0	32.0	36.0
37	33.85007449724013	36.0	36.0	36.0	32.0	36.0
38	33.26669239973752	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	1.0
10	1.0
11	6.0
12	6.0
13	4.0
14	147.0
15	271.0
16	320.0
17	380.0
18	477.0
19	619.0
20	783.0
21	1068.0
22	1412.0
23	2069.0
24	2979.0
25	4384.0
26	6201.0
27	8678.0
28	12190.0
29	16263.0
30	22096.0
31	29670.0
32	40661.0
33	59571.0
34	133822.0
35	303593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.74868054503462	17.35234008082145	11.596671912537218	25.30230746160672
2	16.070082311647223	20.24591531540439	38.44547162349219	25.2385307494562
3	18.47890708121071	25.12666251793195	28.875348022736713	27.519082378120626
4	12.160332275671461	16.041565339571225	36.56036006824621	35.2377423165111
5	13.820884645314294	37.51420472090811	33.953914944601586	14.710995689176002
6	32.82432847642854	36.32163960294533	17.521758799633762	13.332273120992358
7	29.156135407418844	31.564905237966574	21.334156791600726	17.944802563013855
8	28.003792037031033	33.090155062817196	19.824664606985316	19.08138829316646
9	27.962481182691935	14.197089589686184	18.76481259891149	39.075616628710385
10	15.617294529801304	27.49396686290767	32.3454891588811	24.543249448409924
11	36.491128928909454	21.8379197952034	22.46864515252437	19.202306123362778
12	24.349157969987388	24.781482905408847	29.445342548030528	21.424016576573237
13	29.923390166577356	20.171040216688002	25.253911950423664	24.651657666310978
14	23.50001852972786	19.999907351360697	26.367030672876187	30.133043446035256
15	24.899459459459457	27.20787644787645	23.64030888030888	24.252355212355212
16	24.667617874051494	26.474603071658652	24.692633392938212	24.16514566135164
17	23.310132026606087	26.223242180562483	26.034522432477093	24.432103360354336
18	24.014069609982137	25.417170421084652	27.159813290469813	23.408946678463398
19	24.63134131540507	25.93396106964291	26.56226355660281	22.87243405834921
20	25.266892924751172	24.449152149812086	26.69871131712469	23.58524360831205
21	26.31914581515823	24.690450639634978	25.49646019748778	23.49394334771901
22	25.113222434795766	24.886391535764083	26.000009264706563	24.000376764733584
23	23.693227528423876	25.059951482920965	26.441653657233477	24.80516733142168
24	23.71388421328968	25.81913924363389	26.438531741742207	24.02844480133422
25	24.19267909621446	25.121266078082844	26.31840665407548	24.367648171627213
26	23.848808926296282	25.645959615458864	26.761746650708467	23.74348480753639
27	24.56613615272131	25.677760260403236	25.91293230040873	23.84317128646672
28	23.722085056906877	25.53714503649421	26.608578571020498	24.13219133557842
29	23.58629251931784	25.972686337609712	26.715133696115434	23.725887446957017
30	23.93411944902935	25.766879690947526	26.814193315288804	23.484807544734327
31	24.02954146799417	25.720625293376486	25.894952689181512	24.354880549447834
32	23.562283053129594	26.039028819191383	25.966919076501178	24.43176905117784
33	23.56067589748816	25.652357194806157	26.236811181986774	24.55015572571891
34	23.813325468238244	25.924856428557092	26.61557443247008	23.646243670734588
35	24.574367051956177	25.690702863805175	26.306548377382967	23.42838170685568
36	23.657169330553625	26.239981468612463	26.073507837232647	24.02934136360127
37	24.48182308097349	26.184707700812375	25.886069792327753	23.447399425886378
38	23.555897312370877	26.26082015459349	26.308995512897717	23.87428702013792
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	531.0
1	404.0
2	277.0
3	277.0
4	594.0
5	911.0
6	911.0
7	1035.0
8	1159.0
9	1098.5
10	1038.0
11	1038.0
12	1367.5
13	1697.0
14	2028.5
15	2360.0
16	2360.0
17	3526.5
18	4693.0
19	4693.0
20	5481.0
21	6269.0
22	6328.0
23	6387.0
24	6387.0
25	7277.0
26	8167.0
27	8167.0
28	10308.0
29	12449.0
30	15106.0
31	17763.0
32	17763.0
33	20966.0
34	24169.0
35	24169.0
36	26696.5
37	29224.0
38	33158.0
39	37092.0
40	37092.0
41	40223.5
42	43355.0
43	48352.5
44	53350.0
45	53350.0
46	56047.0
47	58744.0
48	58744.0
49	60256.5
50	61769.0
51	60529.0
52	59289.0
53	59289.0
54	55784.0
55	52279.0
56	52279.0
57	49599.5
58	46920.0
59	41659.5
60	36399.0
61	36399.0
62	33236.5
63	30074.0
64	24836.5
65	19599.0
66	19599.0
67	16109.5
68	12620.0
69	12620.0
70	10084.0
71	7548.0
72	5963.0
73	4378.0
74	4378.0
75	3348.0
76	2318.0
77	2318.0
78	2056.0
79	1794.0
80	1404.0
81	1014.0
82	1014.0
83	988.5
84	963.0
85	963.0
86	640.5
87	318.0
88	274.5
89	231.0
90	231.0
91	179.0
92	127.0
93	98.0
94	69.0
95	69.0
96	67.0
97	65.0
98	65.0
99	165.5
100	266.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12614351333616397
2	0.05851700312656811
3	0.013587061411973599
4	0.003087968502721272
5	4.6319527540819084E-4
6	9.263905508163817E-4
7	0.0
8	3.087968502721272E-4
9	0.0
10	3.087968502721272E-4
11	0.0012351874010885088
12	0.0021615779519048904
13	0.01667502991469487
14	0.010344694484116262
15	0.027019724398811132
16	0.011888678735476898
17	0.023777357470953796
18	0.005867140155170417
19	0.008800710232755625
20	0.005712741730034354
21	0.006175937005442544
22	0.008646311807619562
23	0.010499092909252326
24	0.01621183463928668
25	0.020380592117960398
26	0.02315976377040954
27	0.00988149920870807
28	0.005867140155170417
29	0.013432662986837535
30	0.002779171652449145
31	0.006639132280850735
32	0.007874319681939244
33	0.009572702358435945
34	0.013587061411973599
35	0.016983826764966996
36	0.01929980314200795
37	0.010499092909252326
38	0.006330335430578609
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	647675.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.31558980336109
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.43689402094748	54.66851248556303
2	12.110205111964904	16.061907892792618
3	2.9123192414264643	5.7939650457261935
4	1.003780158043757	2.6626509285433104
5	0.4506179305707043	1.4941496920883135
6	0.23280353527158332	0.9263102249905574
7	0.13785152315659244	0.639919354439478
8	0.09448122055086919	0.5012462292937865
9	0.07016857665220723	0.4187943491718127
>10	0.4306643282506438	5.654453076959065
>50	0.0695610144845191	3.2710337612124865
>100	0.04855250896545684	6.194841668986227
>500	0.001400553143203792	0.7060314563559855
>1k	7.00276571601896E-4	1.0061838338771993
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3093	0.47755432894584476	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2157	0.3330374030184892	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1250	0.1929980314200795	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	981	0.1514648550584784	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	852	0.1315474582159262	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	830	0.1281506928629328	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	824	0.12722430231211643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.0879685027212725E-4	0.0
8	0.0	0.0	0.0	6.175937005442545E-4	0.0
9	0.0	0.0	0.0	7.71992125680318E-4	0.0
10	0.0	0.0	0.0	7.71992125680318E-4	0.0
11	0.0	0.0	0.0	7.71992125680318E-4	0.0
12	0.0	0.0	0.0	7.71992125680318E-4	7.71992125680318E-4
13	0.0	0.0	0.0	7.71992125680318E-4	0.0010807889759524452
14	0.0	0.0	0.0	7.71992125680318E-4	0.0010807889759524452
15	0.0	0.0	0.0	9.263905508163817E-4	0.0010807889759524452
16	0.0	0.0	0.0	9.263905508163817E-4	0.0010807889759524452
17	0.0	0.0	0.0	0.0010807889759524452	0.0010807889759524452
18	0.0	0.0	0.0	0.001235187401088509	0.0010807889759524452
19	0.0	0.0	0.0	0.0013895858262245725	0.0010807889759524452
20	0.0	0.0	0.0	0.0013895858262245725	0.0010807889759524452
21	0.0	0.0	0.0	0.001543984251360636	0.0010807889759524452
22	0.0	0.0	0.0	0.002315976377040954	0.0010807889759524452
23	0.0	0.0	0.0	0.0038599606284015903	0.0010807889759524452
24	0.0	0.0	0.0	0.005867140155170417	0.0010807889759524452
25	0.0	0.0	0.0	0.007102327556258926	0.0010807889759524452
26	0.0	0.0	0.0	0.008646311807619562	0.001235187401088509
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATCGT	80	5.2514224E-9	21.996868	27
TAACCTA	40	0.0044850963	19.997154	5
TAGCGTA	50	7.1981904E-4	19.197268	7
CGCAAGA	80	2.7797068E-6	18.00856	2
TCTTTCG	80	2.7920378E-6	18.000217	10
CGAACCC	100	6.799564E-8	17.600212	10
CATCGTT	100	6.8115696E-8	17.597494	28
AATAGCG	55	0.0013650756	17.45206	5
TCCGGAA	120	1.6443664E-9	17.341576	2
AAGACGG	120	1.6570993E-9	17.330866	5
ACCGTCG	150	5.456968E-12	17.064238	8
ATCGTTT	105	1.1886368E-7	16.75952	29
CAAGACG	115	1.8397259E-8	16.693277	4
GATATAA	60	0.0024151192	16.02494	1
GCGACGA	70	3.6929466E-4	16.000193	13
CGTCGTA	150	1.1459633E-10	16.000193	10
ACGACCC	60	0.0024401646	16.000193	16
TCGTTTA	110	2.0166772E-7	15.998959	30
ATATACC	80	5.6179124E-5	15.998959	3
ATACCGT	170	3.6379788E-12	15.997723	6
>>END_MODULE