FastQCFastQC Report
Thu 2 Feb 2017
SRR4062768_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062768_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647675
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34600.5342185509707801No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28030.4327787856563863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16390.25305901879800824No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11270.1740070251283437No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10330.1594935731655537No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9560.14760489443007682No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6920.10684371019415602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA756.3833795E-821.3359079
CGTTTAT751.5069782E-619.1993531
ATACGCT852.3889515E-718.82588
ACGCTAT904.353933E-717.77992410
GTGTTAC753.2311083E-517.066091
AACCGCG854.9714454E-616.943227
ATCGTTT854.9787614E-616.94060529
ACAGTAT957.669951E-716.8441378
ATATACG957.669951E-716.8441376
TATACGC957.669951E-716.8441377
CGCTATT957.6761535E-716.84283811
CAAGACG1950.016.4109694
TATTCCC703.6898957E-416.001935
ATTACAC600.00244091115.9994593
TCTAGCG908.571969E-615.99945928
GAGCGAA1753.6379788E-1215.54233216
GACGGAC1652.910383E-1115.5170247
TAATATA1451.2150849E-915.4489474
TTTCGTT1255.1266397E-815.35948128
AGAACCG951.4288393E-515.1597245
Produced by FastQC (version 0.11.5)