##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062768_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	647675
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11842204809511	32.0	32.0	32.0	32.0	32.0
2	31.29504767051376	32.0	32.0	32.0	32.0	32.0
3	31.378124831126723	32.0	32.0	32.0	32.0	32.0
4	31.484318523951057	32.0	32.0	32.0	32.0	32.0
5	31.399348438645927	32.0	32.0	32.0	32.0	32.0
6	34.907388736634886	36.0	36.0	36.0	36.0	36.0
7	34.94428532828965	36.0	36.0	36.0	36.0	36.0
8	34.89523603659243	36.0	36.0	36.0	36.0	36.0
9	34.99360172926236	36.0	36.0	36.0	36.0	36.0
10	34.83756050488285	36.0	36.0	36.0	32.0	36.0
11	35.0214968927317	36.0	36.0	36.0	36.0	36.0
12	34.92591037171421	36.0	36.0	36.0	32.0	36.0
13	34.97491025591539	36.0	36.0	36.0	36.0	36.0
14	34.9203937159841	36.0	36.0	36.0	32.0	36.0
15	34.88566024626549	36.0	36.0	36.0	32.0	36.0
16	34.907211178445976	36.0	36.0	36.0	32.0	36.0
17	34.8728544408847	36.0	36.0	36.0	32.0	36.0
18	34.865166943297176	36.0	36.0	36.0	32.0	36.0
19	34.85564210445053	36.0	36.0	36.0	32.0	36.0
20	34.8296197938781	36.0	36.0	36.0	32.0	36.0
21	34.822221021345584	36.0	36.0	36.0	32.0	36.0
22	34.81000192998032	36.0	36.0	36.0	32.0	36.0
23	34.75651522754468	36.0	36.0	36.0	32.0	36.0
24	34.74449685413209	36.0	36.0	36.0	32.0	36.0
25	34.723115760219244	36.0	36.0	36.0	32.0	36.0
26	34.6647948430926	36.0	36.0	36.0	32.0	36.0
27	34.641028293511404	36.0	36.0	36.0	32.0	36.0
28	34.62050102288956	36.0	36.0	36.0	32.0	36.0
29	34.582622457250935	36.0	36.0	36.0	32.0	36.0
30	34.55243447716833	36.0	36.0	36.0	32.0	36.0
31	34.536455784151	36.0	36.0	36.0	32.0	36.0
32	34.50659049677693	36.0	36.0	36.0	32.0	36.0
33	34.45092368857838	36.0	36.0	36.0	32.0	36.0
34	34.43123788937353	36.0	36.0	36.0	32.0	36.0
35	34.38550970780098	36.0	36.0	36.0	32.0	36.0
36	34.358272281622725	36.0	36.0	36.0	32.0	36.0
37	34.34197707183387	36.0	36.0	36.0	32.0	36.0
38	33.94572123364342	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	5.0
21	14.0
22	53.0
23	158.0
24	429.0
25	1043.0
26	2302.0
27	4491.0
28	7797.0
29	12157.0
30	18393.0
31	26450.0
32	37535.0
33	59030.0
34	142996.0
35	334820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.12430223885782	18.481115861934786	12.214197808238639	26.18038409096875
2	15.266607480603698	21.24614968927317	37.57918709229166	25.908055737831475
3	17.68942756783881	25.676303701702242	29.168332883004595	27.465935847454354
4	11.821379274141004	16.823123979038836	36.59464483675429	34.76085191006587
5	13.286447678233682	38.094569035395835	33.70147064499942	14.917512641371058
6	32.91697224688308	36.60832979503609	17.16771528930405	13.30698266877678
7	29.009680855991476	31.509410656661572	21.439622029737365	18.041286457609583
8	27.871044444272886	33.48474906008507	19.65738460469533	18.986821890946707
9	27.657882587636205	14.034762656933001	18.666901361694375	39.64045339373642
10	15.264765280977292	27.787384894730017	32.41861571051835	24.529234113774343
11	36.53446481565547	21.64948135246724	22.457793300661162	19.358260531216125
12	24.0886246960281	24.899216427992435	29.485621646659204	21.52653722932026
13	29.847577639367955	20.14871679270744	25.306095350438646	24.69761021748596
14	23.388119041185778	19.966649940170612	26.330026633728338	30.315204384915273
15	24.851970509900802	27.39722854826881	23.58219786158181	24.168603080248584
16	24.70548454932574	26.293629202345624	24.68572152039452	24.315164727934118
17	23.190643455436756	26.275832786505575	26.218242173929823	24.315281584127842
18	24.0980429999614	25.13313004207357	27.326977264831896	23.44184969313313
19	24.54564403443085	25.642644845022584	26.922607789400548	22.88910333114602
20	25.054232446829044	24.373952985679544	26.91010151696453	23.661713050526885
21	26.094146129071106	24.72972513949919	25.653492343370278	23.522636388059425
22	24.94032491654752	24.821437945633143	26.265683044247567	23.972554093571766
23	23.622450870193553	24.940247532701736	26.525772304499807	24.911529292604897
24	23.781835025282742	25.673987725325205	26.421893696684297	24.122283552707763
25	24.149069051820902	24.96012648358045	26.58007976247273	24.310724702125917
26	23.848535144941522	25.52962519782298	26.86393638784884	23.757903269386652
27	24.483884664376422	25.32489288609256	26.25498899911221	23.936233450418808
28	23.691203149727873	25.20492530976184	26.804956189446866	24.298915351063417
29	23.615393522986068	25.484540857683253	26.955031458679123	23.945034160651563
30	23.975141853553094	25.505230246651482	27.035009842899605	23.48461805689582
31	23.921411201605743	25.410738410468213	26.249276257382174	24.418574130543867
32	23.73196433396379	25.558960898598837	26.196626394410778	24.512448373026597
33	23.5872930096113	25.218357972748677	26.573667348593045	24.620681669046977
34	23.840197629984175	25.474196163199135	26.937121241363336	23.748484965453354
35	24.71316632570348	25.17898637433898	26.56563863048597	23.54220866947157
36	23.7833790095341	25.79874165283514	26.349635233720615	24.068244103910143
37	24.550121588759794	25.767244374107385	26.02277376770757	23.659860269425252
38	23.66660902432095	25.688774564903223	26.56313689645376	24.081479514322066
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	286.0
1	213.0
2	140.0
3	140.0
4	507.0
5	874.0
6	874.0
7	1035.0
8	1196.0
9	1181.5
10	1167.0
11	1167.0
12	1444.0
13	1721.0
14	2143.5
15	2566.0
16	2566.0
17	3965.5
18	5365.0
19	5365.0
20	5883.0
21	6401.0
22	6551.5
23	6702.0
24	6702.0
25	7390.0
26	8078.0
27	8078.0
28	10197.0
29	12316.0
30	14886.0
31	17456.0
32	17456.0
33	20722.5
34	23989.0
35	23989.0
36	26533.5
37	29078.0
38	33017.5
39	36957.0
40	36957.0
41	39719.5
42	42482.0
43	47563.5
44	52645.0
45	52645.0
46	56089.5
47	59534.0
48	59534.0
49	61290.0
50	63046.0
51	61171.0
52	59296.0
53	59296.0
54	55909.0
55	52522.0
56	52522.0
57	50096.0
58	47670.0
59	41885.0
60	36100.0
61	36100.0
62	32917.0
63	29734.0
64	24773.5
65	19813.0
66	19813.0
67	16172.5
68	12532.0
69	12532.0
70	10075.0
71	7618.0
72	5924.0
73	4230.0
74	4230.0
75	3201.0
76	2172.0
77	2172.0
78	1964.5
79	1757.0
80	1352.0
81	947.0
82	947.0
83	904.0
84	861.0
85	861.0
86	549.5
87	238.0
88	187.5
89	137.0
90	137.0
91	80.5
92	24.0
93	18.5
94	13.0
95	13.0
96	9.0
97	5.0
98	5.0
99	6.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010499092909252326
2	0.0
3	0.0
4	1.543984251360636E-4
5	0.0
6	0.0
7	7.71992125680318E-4
8	0.001543984251360636
9	0.004014359053537654
10	0.0010807889759524452
11	0.004477554328945845
12	0.0
13	1.543984251360636E-4
14	0.0
15	0.0
16	1.543984251360636E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.543984251360636E-4
22	1.543984251360636E-4
23	4.6319527540819084E-4
24	0.0
25	3.087968502721272E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.6319527540819084E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	647675.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.6932354581111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.43000198269002	52.679702916206196
2	12.746296646276631	16.491983403130124
3	3.1148318197839107	6.045256449890918
4	1.0620154153122179	2.7482085329174786
5	0.4906981802266271	1.5872426456133915
6	0.24082193386684797	0.9347730042675395
7	0.1483971482015157	0.6720204154939006
8	0.10332949649328284	0.5347775557126411
9	0.07470358504052739	0.43495349549326784
>10	0.46440107700358707	5.953248729599316
>50	0.07004783691374653	3.2965974296166434
>100	0.05229587420999231	6.642069679385528
>500	9.595573249426025E-4	0.4176221291878958
>1k	0.001199446656178253	1.561543613485176
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3460	0.5342185509707801	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2803	0.4327787856563863	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1639	0.25305901879800824	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1127	0.1740070251283437	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1033	0.1594935731655537	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	956	0.14760489443007682	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	692	0.10684371019415602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.5439842513606362E-4	0.0
3	0.0	0.0	0.0	1.5439842513606362E-4	0.0
4	0.0	0.0	0.0	1.5439842513606362E-4	0.0
5	0.0	0.0	0.0	3.0879685027212725E-4	0.0
6	0.0	0.0	0.0	3.0879685027212725E-4	0.0
7	0.0	0.0	0.0	3.0879685027212725E-4	0.0
8	0.0	0.0	0.0	0.0010807889759524452	0.0
9	0.0	0.0	0.0	0.0010807889759524452	0.0
10	0.0	0.0	0.0	0.0013895858262245725	0.0
11	0.0	0.0	0.0	0.0013895858262245725	0.0
12	0.0	0.0	0.0	0.001543984251360636	4.6319527540819084E-4
13	0.0	0.0	0.0	0.001543984251360636	4.6319527540819084E-4
14	0.0	0.0	0.0	0.001543984251360636	4.6319527540819084E-4
15	0.0	0.0	0.0	0.0016983826764966998	4.6319527540819084E-4
16	0.0	0.0	0.0	0.0016983826764966998	4.6319527540819084E-4
17	0.0	0.0	0.0	0.0018527811016327634	4.6319527540819084E-4
18	0.0	0.0	0.0	0.002007179526768827	6.175937005442545E-4
19	0.0	0.0	0.0	0.002007179526768827	6.175937005442545E-4
20	0.0	0.0	0.0	0.0021615779519048904	7.71992125680318E-4
21	0.0	0.0	0.0	0.002315976377040954	7.71992125680318E-4
22	0.0	0.0	0.0	0.0029335700775852086	7.71992125680318E-4
23	0.0	0.0	0.0	0.004323155903809781	7.71992125680318E-4
24	0.0	0.0	0.0	0.006484733855714671	7.71992125680318E-4
25	0.0	0.0	0.0	0.007874319681939244	7.71992125680318E-4
26	0.0	0.0	0.0	0.00941830393329988	7.71992125680318E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTA	75	6.3833795E-8	21.335907	9
CGTTTAT	75	1.5069782E-6	19.19935	31
ATACGCT	85	2.3889515E-7	18.8258	8
ACGCTAT	90	4.353933E-7	17.779924	10
GTGTTAC	75	3.2311083E-5	17.06609	1
AACCGCG	85	4.9714454E-6	16.94322	7
ATCGTTT	85	4.9787614E-6	16.940605	29
ACAGTAT	95	7.669951E-7	16.844137	8
ATATACG	95	7.669951E-7	16.844137	6
TATACGC	95	7.669951E-7	16.844137	7
CGCTATT	95	7.6761535E-7	16.842838	11
CAAGACG	195	0.0	16.410969	4
TATTCCC	70	3.6898957E-4	16.00193	5
ATTACAC	60	0.002440911	15.999459	3
TCTAGCG	90	8.571969E-6	15.999459	28
GAGCGAA	175	3.6379788E-12	15.542332	16
GACGGAC	165	2.910383E-11	15.517024	7
TAATATA	145	1.2150849E-9	15.448947	4
TTTCGTT	125	5.1266397E-8	15.359481	28
AGAACCG	95	1.4288393E-5	15.159724	5
>>END_MODULE