FastQCFastQC Report
Thu 2 Feb 2017
SRR4062767_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062767_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134864
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39610.3490286060708596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26700.235270481749355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15320.13499414907865612No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13110.11552045002749228No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12780.11261261261261261No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA12020.10591577492985943No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11580.10203865837668655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA1700.020.7016077
TAGCGGC2800.020.569930
GGACCGT400.00448880719.9958696
GGGCGTA659.4504885E-619.688247
CTAGCGG2850.019.64766129
AATAGCG2000.019.1960355
TCGTTTA2600.018.46016530
ATCGTTT2600.018.46016529
ATAGCGT2000.018.39626
TCTAGCG3050.018.35767428
AGCGTAT1950.018.048358
CTATACT802.8016384E-617.9962834
CGTATAT2150.017.8607110
GCGATTT2600.017.84325427
CGACGAA450.00885399917.77645532
GCATCGT2700.017.77488927
TACGACT450.00886074417.7741054
ATTAGAC550.001366117917.451713
CATCGTT2750.017.4517128
CGATTTG2750.016.86998628
Produced by FastQC (version 0.11.5)