##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062767_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1134864
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229642494607283	32.0	32.0	32.0	32.0	32.0
2	30.890944641824923	32.0	32.0	32.0	32.0	32.0
3	30.952935329695894	32.0	32.0	32.0	32.0	32.0
4	31.004443704267647	32.0	32.0	32.0	32.0	32.0
5	30.952083245217047	32.0	32.0	32.0	32.0	32.0
6	34.59913875142748	36.0	36.0	36.0	32.0	36.0
7	34.51262353903199	36.0	36.0	36.0	32.0	36.0
8	34.4962471274091	36.0	36.0	36.0	32.0	36.0
9	34.61684660012125	36.0	36.0	36.0	32.0	36.0
10	34.36609320588194	36.0	36.0	36.0	32.0	36.0
11	34.62222698050163	36.0	36.0	36.0	32.0	36.0
12	34.45169377123602	36.0	36.0	36.0	32.0	36.0
13	34.53380493169225	36.0	36.0	36.0	32.0	36.0
14	34.44831186820623	36.0	36.0	36.0	32.0	36.0
15	34.40084714996687	36.0	36.0	36.0	32.0	36.0
16	34.41382932228003	36.0	36.0	36.0	32.0	36.0
17	34.338772751624866	36.0	36.0	36.0	32.0	36.0
18	34.37238647097802	36.0	36.0	36.0	32.0	36.0
19	34.35943602052757	36.0	36.0	36.0	32.0	36.0
20	34.32137859690677	36.0	36.0	36.0	32.0	36.0
21	34.30654333911376	36.0	36.0	36.0	32.0	36.0
22	34.28952984674816	36.0	36.0	36.0	32.0	36.0
23	34.23688829674745	36.0	36.0	36.0	32.0	36.0
24	34.2310761465691	36.0	36.0	36.0	32.0	36.0
25	34.20908848989835	36.0	36.0	36.0	32.0	36.0
26	34.17171220516291	36.0	36.0	36.0	32.0	36.0
27	34.17025828645547	36.0	36.0	36.0	32.0	36.0
28	34.150842744152605	36.0	36.0	36.0	32.0	36.0
29	34.119190493310214	36.0	36.0	36.0	32.0	36.0
30	34.093293117060725	36.0	36.0	36.0	32.0	36.0
31	34.11172087580538	36.0	36.0	36.0	32.0	36.0
32	34.06667054378322	36.0	36.0	36.0	32.0	36.0
33	34.024469011264785	36.0	36.0	36.0	32.0	36.0
34	34.01883309365704	36.0	36.0	36.0	32.0	36.0
35	33.98617543599938	36.0	36.0	36.0	32.0	36.0
36	33.93821197958522	36.0	36.0	36.0	32.0	36.0
37	33.933989447193674	36.0	36.0	36.0	32.0	36.0
38	33.34583703421732	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	7.0
10	5.0
11	13.0
12	7.0
13	12.0
14	139.0
15	289.0
16	350.0
17	496.0
18	638.0
19	839.0
20	1078.0
21	1474.0
22	2135.0
23	3221.0
24	4890.0
25	7193.0
26	10392.0
27	14966.0
28	20898.0
29	27926.0
30	38315.0
31	51633.0
32	70537.0
33	104989.0
34	237920.0
35	534497.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.051904878698636	17.117969964480377	11.198385824618114	25.63173933220287
2	16.590887249573054	19.405702456240583	37.894822224749674	26.108588069436696
3	19.245153990137016	23.684994377606305	28.373788717847486	28.696062914409193
4	12.275760945615145	15.031572788362743	36.36113700810159	36.331529257920515
5	14.212841925134445	37.03625377909718	33.817709834067784	14.933194461700596
6	34.22236869119885	35.797008097012586	16.77286157007498	13.207761641713581
7	30.338437028577875	30.648606352831703	20.49637665834849	18.516579960241934
8	28.502697687205746	33.10643329887977	19.130007992168206	19.260861021746276
9	27.562494437644787	13.965322625414037	18.549320137003562	39.92286279993761
10	15.585975362599791	26.944645154468393	32.099289780237214	25.370089702694603
11	37.507313806351554	21.395527122766207	21.97032180747947	19.12683726340277
12	24.36338706806458	24.19261319276652	28.972871803276956	22.471127935891946
13	29.715424334211388	19.587787860654775	25.202365015814653	25.49442278931918
14	23.23918093405102	19.853959793999767	25.630620150939937	31.276239121009276
15	25.202630762505112	27.246213563480982	22.74215213436561	24.80900353964829
16	25.25651227901345	26.12666679004284	24.211956737264277	24.404864193679437
17	23.619994447455746	26.098191897692168	25.652490051691544	24.629323603160543
18	24.486028251923265	25.223080922461428	26.68687598586523	23.604014839750086
19	25.116367603541175	25.389199182915245	25.937594182412937	23.556839031130647
20	25.361256267679483	24.46928506595934	25.826011861225425	24.343446805135752
21	26.495933619611684	24.38189167539807	24.978828737928417	24.143345967061837
22	25.247580592571968	24.60613580405752	25.69815171548368	24.448131887886838
23	23.98939338928623	24.667155461399904	26.125520372517986	25.217930776795882
24	24.233635437487	25.527007829327015	25.88348720551895	24.35586952766703
25	24.530879299465223	24.870271337368553	25.868441740197202	24.730407622969025
26	24.050895079355005	25.889350706122077	26.182745662090802	23.87700855243212
27	24.947632329160662	25.190149502319866	25.478050521473612	24.384167647045864
28	23.877087932181585	25.334179301107245	26.205261743310466	24.5834710234007
29	23.88724728592063	25.555131568627274	26.2327370980435	24.32488404740859
30	24.016873115895606	25.66127052412579	26.314588266187766	24.007268093790838
31	24.4447949693604	25.47395766324817	25.411920627843	24.669326739548435
32	24.051315583081156	25.550370027018403	25.415630491142792	24.982683898757646
33	23.870094460252933	25.26289630521968	25.914221736168695	24.95278749835869
34	24.519268053665723	25.466896031471858	26.048709470463404	23.96512644439901
35	24.976491314344837	25.272431957006646	26.018364535770655	23.73271219287786
36	23.984193025577934	25.866823420338086	25.64094655011197	24.508037003972014
37	24.832628774720135	25.745250287948124	25.37459693871706	24.047523998614675
38	23.964278821877897	25.82347907848948	25.807528976752508	24.40471312288011
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	98.5
2	141.0
3	141.0
4	426.0
5	711.0
6	711.0
7	952.0
8	1193.0
9	1258.5
10	1324.0
11	1324.0
12	1737.5
13	2151.0
14	2714.5
15	3278.0
16	3278.0
17	4796.5
18	6315.0
19	6315.0
20	7580.5
21	8846.0
22	9358.0
23	9870.0
24	9870.0
25	11715.0
26	13560.0
27	13560.0
28	17312.5
29	21065.0
30	25313.5
31	29562.0
32	29562.0
33	35327.0
34	41092.0
35	41092.0
36	45989.0
37	50886.0
38	58100.5
39	65315.0
40	65315.0
41	70008.0
42	74701.0
43	82406.5
44	90112.0
45	90112.0
46	93590.0
47	97068.0
48	97068.0
49	100782.5
50	104497.0
51	103294.5
52	102092.0
53	102092.0
54	97507.5
55	92923.0
56	92923.0
57	89081.5
58	85240.0
59	76601.5
60	67963.0
61	67963.0
62	63084.5
63	58206.0
64	48828.0
65	39450.0
66	39450.0
67	32983.0
68	26516.0
69	26516.0
70	21226.0
71	15936.0
72	12629.0
73	9322.0
74	9322.0
75	7104.0
76	4886.0
77	4886.0
78	4405.0
79	3924.0
80	3113.0
81	2302.0
82	2302.0
83	2178.0
84	2054.0
85	2054.0
86	1400.5
87	747.0
88	619.5
89	492.0
90	492.0
91	354.5
92	217.0
93	185.5
94	154.0
95	154.0
96	157.5
97	161.0
98	161.0
99	348.5
100	536.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12389149713093375
2	0.057363701729898915
3	0.01022148909472853
4	0.002291023417783981
5	2.643488558981517E-4
6	8.81162852993839E-5
7	0.0
8	2.643488558981517E-4
9	2.643488558981517E-4
10	3.524651411975356E-4
11	0.0021147908471852135
12	0.0026434885589815167
13	0.013834256792003272
14	0.00951655881233346
15	0.02537749016622256
16	0.010838303091824219
17	0.02194095503954659
18	0.006520605112154408
19	0.008635395959339622
20	0.006520605112154408
21	0.006256256256256256
22	0.007225535394549478
23	0.008811628529938388
24	0.013746140506703888
25	0.016565861636284172
26	0.01903311762466692
27	0.008723512244639004
28	0.006080023685657489
29	0.011719465944818059
30	0.003612767697274739
31	0.005110744547364265
32	0.007225535394549478
33	0.008899744815237772
34	0.01198381480071621
35	0.015596582497990948
36	0.015420349927392182
37	0.009780907668231613
38	0.006608721397453793
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1134864.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.01416448773986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.02049323186716	42.58224412328922
2	14.255207368941313	15.969870607414402
3	4.7318998308629405	7.9516024639639555
4	2.002013485404569	4.485644507124996
5	0.975888519321976	2.733179002149903
6	0.5251657491321223	1.7650032393128294
7	0.309644875699698	1.2141149300160055
8	0.20945143427903679	0.9385797673519201
9	0.14537181174792121	0.7328592517615932
>10	0.6768056301035313	6.970858309153708
>50	0.0658224121226076	2.5966814407304026
>100	0.07448652499294417	8.256103983668842
>500	0.006483962173298555	2.553074709993121
>1k	0.001265163350887523	1.2501836640690092
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3961	0.3490286060708596	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2670	0.235270481749355	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1532	0.13499414907865612	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1311	0.11552045002749228	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1278	0.11261261261261261	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1202	0.10591577492985943	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1158	0.10203865837668655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	8.811628529938388E-5
17	0.0	0.0	0.0	0.0	8.811628529938388E-5
18	0.0	0.0	0.0	0.0	8.811628529938388E-5
19	0.0	0.0	0.0	1.7623257059876777E-4	8.811628529938388E-5
20	0.0	0.0	0.0	3.5246514119753554E-4	1.7623257059876777E-4
21	0.0	0.0	0.0	3.5246514119753554E-4	1.7623257059876777E-4
22	0.0	0.0	0.0	6.168139970956873E-4	2.643488558981517E-4
23	0.0	0.0	0.0	0.0010573954235926068	2.643488558981517E-4
24	0.0	0.0	0.0	0.0019385582765864455	2.643488558981517E-4
25	0.0	0.0	0.0	0.0026434885589815167	2.643488558981517E-4
26	0.0	0.0	0.0	0.0029959537001790523	3.5246514119753554E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGTA	170	0.0	20.701607	7
TAGCGGC	280	0.0	20.5699	30
GGACCGT	40	0.004488807	19.995869	6
GGGCGTA	65	9.4504885E-6	19.68824	7
CTAGCGG	285	0.0	19.647661	29
AATAGCG	200	0.0	19.196035	5
TCGTTTA	260	0.0	18.460165	30
ATCGTTT	260	0.0	18.460165	29
ATAGCGT	200	0.0	18.3962	6
TCTAGCG	305	0.0	18.357674	28
AGCGTAT	195	0.0	18.04835	8
CTATACT	80	2.8016384E-6	17.996283	4
CGTATAT	215	0.0	17.86071	10
GCGATTT	260	0.0	17.843254	27
CGACGAA	45	0.008853999	17.776455	32
GCATCGT	270	0.0	17.774889	27
TACGACT	45	0.008860744	17.774105	4
ATTAGAC	55	0.0013661179	17.45171	3
CATCGTT	275	0.0	17.45171	28
CGATTTG	275	0.0	16.869986	28
>>END_MODULE