FastQCFastQC Report
Thu 2 Feb 2017
SRR4062767_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062767_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134864
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42400.3736130496693877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT32460.28602546208180013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19120.168478337492422No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14150.12468454369862822No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13060.11507986860099537No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12980.1143749383186003No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11490.1012456118089921No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA400.00447994620.0026998
TCTAGCG3250.019.69149428
CTAGCGG3300.018.90830829
TAGCGGC3450.018.08620830
CGCAAGA3200.017.9992562
CAAGACG3500.017.828624
AGGTATT550.00136500917.45382515
ATGCTCG550.00136500917.45382525
TAGGACG1201.6589183E-917.333384
GGTCCAA4200.017.14441711
AAGACGG3600.016.8911695
GTACTAC957.6884135E-716.8428941
GCGCAAG3450.016.2326431
TACCGTC1304.791218E-916.002167
ATAGGCC1001.3142944E-616.0000443
CGTGATT908.583973E-615.99933928
CGGCATC2400.015.99933925
CGGTCCA4050.015.803905510
GACGGAC3650.015.7829517
GCATTCG2950.015.72816322
Produced by FastQC (version 0.11.5)