##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062767_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1134864
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.153086184776324	32.0	32.0	32.0	32.0	32.0
2	31.2862466339579	32.0	32.0	32.0	32.0	32.0
3	31.383060877779187	32.0	32.0	32.0	32.0	32.0
4	31.48225514246641	32.0	32.0	32.0	32.0	32.0
5	31.399725429655007	32.0	32.0	32.0	32.0	32.0
6	34.934982517729	36.0	36.0	36.0	36.0	36.0
7	34.96689911742729	36.0	36.0	36.0	36.0	36.0
8	34.89990606803987	36.0	36.0	36.0	36.0	36.0
9	35.01142691987762	36.0	36.0	36.0	36.0	36.0
10	34.860384151757394	36.0	36.0	36.0	32.0	36.0
11	35.03186989806708	36.0	36.0	36.0	36.0	36.0
12	34.93464327003059	36.0	36.0	36.0	36.0	36.0
13	34.992804424142456	36.0	36.0	36.0	36.0	36.0
14	34.93552443288359	36.0	36.0	36.0	32.0	36.0
15	34.90053257482835	36.0	36.0	36.0	32.0	36.0
16	34.918891602870474	36.0	36.0	36.0	32.0	36.0
17	34.89105831183296	36.0	36.0	36.0	32.0	36.0
18	34.89109091485852	36.0	36.0	36.0	32.0	36.0
19	34.870581849455085	36.0	36.0	36.0	32.0	36.0
20	34.85733444712318	36.0	36.0	36.0	32.0	36.0
21	34.85302379844634	36.0	36.0	36.0	32.0	36.0
22	34.8408884236349	36.0	36.0	36.0	32.0	36.0
23	34.78123810430149	36.0	36.0	36.0	32.0	36.0
24	34.76870884969477	36.0	36.0	36.0	32.0	36.0
25	34.74562943224915	36.0	36.0	36.0	32.0	36.0
26	34.686185305023336	36.0	36.0	36.0	32.0	36.0
27	34.67228584218021	36.0	36.0	36.0	32.0	36.0
28	34.64154823837922	36.0	36.0	36.0	32.0	36.0
29	34.61512216441794	36.0	36.0	36.0	32.0	36.0
30	34.584304374797334	36.0	36.0	36.0	32.0	36.0
31	34.577924755741655	36.0	36.0	36.0	32.0	36.0
32	34.55398796684008	36.0	36.0	36.0	32.0	36.0
33	34.514282768684176	36.0	36.0	36.0	32.0	36.0
34	34.48864533547632	36.0	36.0	36.0	32.0	36.0
35	34.459005660590165	36.0	36.0	36.0	32.0	36.0
36	34.428435477731256	36.0	36.0	36.0	32.0	36.0
37	34.426987727163784	36.0	36.0	36.0	32.0	36.0
38	34.00061240818283	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	17.0
22	76.0
23	251.0
24	719.0
25	1777.0
26	3661.0
27	7248.0
28	12767.0
29	20292.0
30	31129.0
31	44818.0
32	64759.0
33	103816.0
34	255192.0
35	588334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.389078308809594	18.323667482998474	11.84524181957099	26.442012388620938
2	15.629287411784507	20.38677796350749	37.071523170638216	26.912411454069783
3	18.28509847876045	24.22052334024165	28.873680018046215	28.620698162951687
4	11.799947306322096	15.84114706558777	36.41256506303415	35.94634056505598
5	13.769403206022924	37.47427004469258	33.54851330203443	15.207813447250068
6	34.326522232198954	36.206044253799796	16.354573195178627	13.112860318822625
7	30.03741437254705	30.84209654423725	20.494987038025904	18.625502045189794
8	28.255877480525886	33.46551055655423	18.960029607698	19.31858235522188
9	27.349984490877816	13.736940614161238	18.51489580689733	40.39817908806362
10	15.330342052810316	27.27496224183904	32.071526381367114	25.323169323983524
11	37.74409243436821	21.22969714914136	21.708981896624653	19.317228519865775
12	24.04415354167658	24.329387465215913	29.018784729014556	22.607674264092953
13	29.764447546137685	19.492732168788507	25.250602715391445	25.492217569682357
14	23.131406053941266	19.7513534661422	25.639812347558827	31.47742813235771
15	25.06000719028888	27.376055633097884	22.6686193235489	24.895317853064334
16	25.314972216886282	26.105817271174818	24.14399283789571	24.435217674043187
17	23.56846282902621	26.168950640781624	25.591348390644164	24.671238139548
18	24.531926292489672	25.087940052728786	26.664340396734765	23.71579325804678
19	25.182312594284422	25.372379421675195	25.922401274513952	23.522906709526428
20	25.333432023572865	24.397196492266914	25.946192671544782	24.32317881261543
21	26.49112712283333	24.408144419194212	25.026016356159293	24.07471210181317
22	25.149709568723654	24.668770883559617	25.666247233148642	24.51527231456809
23	23.96718537969441	24.574925541476482	26.1788238196782	25.279065259150908
24	24.219906526244557	25.466047032948442	25.827411918961214	24.48663452184579
25	24.47791100960115	24.819273498850965	25.922577507084547	24.780237984463334
26	24.17031835560768	25.68644849642644	26.208802295959956	23.934430852005924
27	25.065646632548038	25.017182675633382	25.608795415133446	24.308375276685133
28	23.916345923388178	25.185308547984604	26.259357949498796	24.638987579128425
29	23.96718902000592	25.3865661436084	26.223054039955446	24.423190796430234
30	24.07372160893288	25.45045045045045	26.420786984167265	24.055040956449407
31	24.415524679609188	25.35193644348574	25.493979895388346	24.73855898151673
32	24.088348912292574	25.400664749256297	25.548259527132767	24.962726811318362
33	23.958113042620084	25.08291742446672	25.95412313722173	25.004846395691466
34	24.568935132315413	25.21306517785391	26.07845521577916	24.139544474051515
35	25.108999844915335	25.003172186270778	26.04867191134797	23.839156057465914
36	23.98772011448068	25.77128184522551	25.748107262191773	24.492890778102044
37	25.014384996250648	25.482899698025225	25.415138214921097	24.08757709080303
38	24.085264842307097	25.462786730392363	25.998005047300822	24.453943379999718
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	42.5
2	63.0
3	63.0
4	300.5
5	538.0
6	538.0
7	812.5
8	1087.0
9	1211.0
10	1335.0
11	1335.0
12	1765.5
13	2196.0
14	2848.0
15	3500.0
16	3500.0
17	5159.0
18	6818.0
19	6818.0
20	7832.5
21	8847.0
22	9560.5
23	10274.0
24	10274.0
25	11865.5
26	13457.0
27	13457.0
28	17099.5
29	20742.0
30	24878.0
31	29014.0
32	29014.0
33	35098.0
34	41182.0
35	41182.0
36	45955.0
37	50728.0
38	57622.0
39	64516.0
40	64516.0
41	69594.5
42	74673.0
43	82544.5
44	90416.0
45	90416.0
46	93825.5
47	97235.0
48	97235.0
49	101341.5
50	105448.0
51	104317.0
52	103186.0
53	103186.0
54	98013.5
55	92841.0
56	92841.0
57	89182.5
58	85524.0
59	76646.0
60	67768.0
61	67768.0
62	63074.0
63	58380.0
64	48824.5
65	39269.0
66	39269.0
67	33035.0
68	26801.0
69	26801.0
70	21537.5
71	16274.0
72	12760.5
73	9247.0
74	9247.0
75	7020.5
76	4794.0
77	4794.0
78	4292.5
79	3791.0
80	2954.5
81	2118.0
82	2118.0
83	1955.5
84	1793.0
85	1793.0
86	1182.0
87	571.0
88	435.0
89	299.0
90	299.0
91	186.0
92	73.0
93	46.5
94	20.0
95	20.0
96	18.5
97	17.0
98	17.0
99	12.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00995714023883038
2	8.81162852993839E-5
3	0.0
4	2.643488558981517E-4
5	0.0
6	8.81162852993839E-5
7	5.286977117963034E-4
8	0.0021147908471852135
9	0.004229581694370427
10	8.81162852993839E-4
11	0.004229581694370427
12	5.286977117963034E-4
13	0.0
14	0.0
15	0.0
16	1.762325705987678E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	8.81162852993839E-5
22	0.0
23	3.524651411975356E-4
24	0.0
25	0.0
26	8.81162852993839E-5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	8.81162852993839E-5
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1134864.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.09257678523003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.14711065664356	40.108082765974395
2	15.389317980616129	16.648957290775982
3	5.166091244917152	8.383415620355569
4	2.0626561105263237	4.462975361606763
5	1.0592654920929832	2.864919998349208
6	0.5595767523582097	1.8161369066499664
7	0.3477637031852464	1.3168004372364719
8	0.235539963610832	1.0192770854087372
9	0.15171683260634858	0.7386078975633722
>10	0.720485105689832	7.0879974851089225
>50	0.07369307252275369	2.7984774621136252
>100	0.07792432301898267	8.472227338354077
>500	0.007218250690997194	2.706909567666386
>1k	0.0016405115206811806	1.5752147828365464
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4240	0.3736130496693877	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3246	0.28602546208180013	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1912	0.168478337492422	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1415	0.12468454369862822	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1306	0.11507986860099537	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1298	0.1143749383186003	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1149	0.1012456118089921	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.7623257059876777E-4
13	0.0	0.0	0.0	0.0	5.286977117963034E-4
14	0.0	0.0	0.0	0.0	5.286977117963034E-4
15	0.0	0.0	0.0	0.0	6.168139970956873E-4
16	0.0	0.0	0.0	0.0	7.049302823950711E-4
17	0.0	0.0	0.0	0.0	7.049302823950711E-4
18	0.0	0.0	0.0	0.0	7.93046567694455E-4
19	0.0	0.0	0.0	1.7623257059876777E-4	8.811628529938389E-4
20	0.0	0.0	0.0	3.5246514119753554E-4	8.811628529938389E-4
21	0.0	0.0	0.0	3.5246514119753554E-4	8.811628529938389E-4
22	0.0	0.0	0.0	7.049302823950711E-4	8.811628529938389E-4
23	0.0	0.0	0.0	0.0011455117088919906	8.811628529938389E-4
24	0.0	0.0	0.0	0.002202907132484597	8.811628529938389E-4
25	0.0	0.0	0.0	0.0029959537001790523	8.811628529938389E-4
26	0.0	0.0	0.0	0.003348418841376588	8.811628529938389E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTA	40	0.004479946	20.002699	8
TCTAGCG	325	0.0	19.691494	28
CTAGCGG	330	0.0	18.908308	29
TAGCGGC	345	0.0	18.086208	30
CGCAAGA	320	0.0	17.999256	2
CAAGACG	350	0.0	17.82862	4
AGGTATT	55	0.001365009	17.453825	15
ATGCTCG	55	0.001365009	17.453825	25
TAGGACG	120	1.6589183E-9	17.33338	4
GGTCCAA	420	0.0	17.144417	11
AAGACGG	360	0.0	16.891169	5
GTACTAC	95	7.6884135E-7	16.842894	1
GCGCAAG	345	0.0	16.232643	1
TACCGTC	130	4.791218E-9	16.00216	7
ATAGGCC	100	1.3142944E-6	16.000044	3
CGTGATT	90	8.583973E-6	15.999339	28
CGGCATC	240	0.0	15.999339	25
CGGTCCA	405	0.0	15.8039055	10
GACGGAC	365	0.0	15.782951	7
GCATTCG	295	0.0	15.728163	22
>>END_MODULE