FastQCFastQC Report
Thu 2 Feb 2017
SRR4062766_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062766_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688407
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT27870.165066835188435No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA26790.1586702732220371No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG26400.15636040362306008No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26200.15517585511076415No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT25400.15043766106158052No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG25080.14854238344190707No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24730.14646942354538925No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24580.1455810121611673No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA22980.13610462406280002No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22830.13521621267857808No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG22750.13474239327365972No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20690.12254154359701186No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG20180.1195209448906573No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT19780.11715184786606546No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19510.11555270737446599No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA19430.11507888796954763No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT19280.11419047658532569No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT18990.11247288124249663No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC18850.1116436972838895No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA18430.10915614540806809No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT18300.10838618887507574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17460.10341108512343292No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG17100.10127889780130028No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT16990.10062739611953753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA851.0426447E-820.7310771
TACCGTC4700.017.3606917
ACCGTCG4650.017.2032998
ATACCGT5100.016.938186
ATCGTTT4500.016.7106329
CCTATTC6800.016.4696273
GTATTAT1203.0664523E-816.0194681
CTCGTGA600.002443009815.99954112
CGAGCCG7100.015.99954115
GTCCTAT7350.015.9104931
CCGTCGT5100.015.6853599
AGGCCCG6800.015.52896610
GGTATAT1551.8553692E-1015.5027111
CATCGTT4900.015.34649928
TCTAGCG5650.015.29159628
GTATCAA26300.015.2276311
CGTCGTA5300.015.09390610
CGCTTCG5550.014.990561532
CTAGCGG5850.014.76880729
GAGCCGC7700.014.75282416
Produced by FastQC (version 0.11.5)