##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062766_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1688407
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.289733458816507	32.0	32.0	32.0	32.0	32.0
2	30.94696539400749	32.0	32.0	32.0	32.0	32.0
3	30.989972796843414	32.0	32.0	32.0	32.0	32.0
4	31.057987795596677	32.0	32.0	32.0	32.0	32.0
5	30.990668126820132	32.0	32.0	32.0	32.0	32.0
6	34.64072288257511	36.0	36.0	36.0	32.0	36.0
7	34.575466697306986	36.0	36.0	36.0	32.0	36.0
8	34.57493068910517	36.0	36.0	36.0	32.0	36.0
9	34.687122240076	36.0	36.0	36.0	32.0	36.0
10	34.436041783764225	36.0	36.0	36.0	32.0	36.0
11	34.65458328471749	36.0	36.0	36.0	32.0	36.0
12	34.489090604338884	36.0	36.0	36.0	32.0	36.0
13	34.56769487451781	36.0	36.0	36.0	32.0	36.0
14	34.47657881067776	36.0	36.0	36.0	32.0	36.0
15	34.4265742797797	36.0	36.0	36.0	32.0	36.0
16	34.441451616819876	36.0	36.0	36.0	32.0	36.0
17	34.37505589588292	36.0	36.0	36.0	32.0	36.0
18	34.39260142844705	36.0	36.0	36.0	32.0	36.0
19	34.383103126201206	36.0	36.0	36.0	32.0	36.0
20	34.372428567282654	36.0	36.0	36.0	32.0	36.0
21	34.35093256543002	36.0	36.0	36.0	32.0	36.0
22	34.3285854654713	36.0	36.0	36.0	32.0	36.0
23	34.28545842323563	36.0	36.0	36.0	32.0	36.0
24	34.26000602935193	36.0	36.0	36.0	32.0	36.0
25	34.237354500425546	36.0	36.0	36.0	32.0	36.0
26	34.18618733516267	36.0	36.0	36.0	32.0	36.0
27	34.19796174737489	36.0	36.0	36.0	32.0	36.0
28	34.18695373805013	36.0	36.0	36.0	32.0	36.0
29	34.1522215911211	36.0	36.0	36.0	32.0	36.0
30	34.12670463934347	36.0	36.0	36.0	32.0	36.0
31	34.14461738194642	36.0	36.0	36.0	32.0	36.0
32	34.09911413539508	36.0	36.0	36.0	32.0	36.0
33	34.06595566116464	36.0	36.0	36.0	32.0	36.0
34	34.07844613295254	36.0	36.0	36.0	32.0	36.0
35	34.03384077417353	36.0	36.0	36.0	32.0	36.0
36	34.00545543817338	36.0	36.0	36.0	32.0	36.0
37	33.99658139299351	36.0	36.0	36.0	32.0	36.0
38	33.419750688074615	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	3.0
9	7.0
10	16.0
11	13.0
12	10.0
13	6.0
14	182.0
15	387.0
16	550.0
17	616.0
18	782.0
19	988.0
20	1398.0
21	1906.0
22	2929.0
23	4500.0
24	6992.0
25	10316.0
26	15108.0
27	22029.0
28	30375.0
29	41302.0
30	55297.0
31	74434.0
32	101869.0
33	153844.0
34	353634.0
35	808909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.21295773578819	17.279748378741232	11.289748710820568	26.217545174650013
2	17.11324920799155	19.64649104405262	36.83606699212199	26.40419275583384
3	18.60840503785738	23.462691730374406	28.8451810176415	29.083722214126713
4	12.653484288053827	15.143585658314404	36.007320691912	36.195609361719775
5	14.954427349394663	36.16506248804344	33.51362204402622	15.366888118535682
6	34.5621481140155	35.526077320448564	16.541499003199476	13.37027556233646
7	30.511541352292426	30.319703720726103	20.949510396486154	18.219244530495313
8	28.261388451426473	32.59987716173725	19.51937900934554	19.619355377490745
9	27.49705490230686	13.963050203180646	18.65548368602445	39.88441120848804
10	16.21609135766055	25.884611261450953	31.24217823177843	26.657119149110077
11	38.303506646963186	20.72247410956754	22.00158141160235	18.972437831866927
12	24.878284540537468	23.718641240869857	28.226081579564593	23.176992639028075
13	29.368988974900308	18.715479922376474	25.26674201551049	26.648789087212734
14	23.73518061655743	19.98382936125977	24.392490426448767	31.888499595734032
15	25.3670359044113	27.035892562835386	21.852010270251697	25.74506126250161
16	26.41157156675692	25.26396348315106	23.31479492739931	25.009670022692713
17	24.46996126269744	25.639959313688028	24.793354648032004	25.096724775582523
18	25.7430193672504	24.004717132002696	25.775951629850844	24.476311870896055
19	25.980673798983347	24.660038216454875	24.890035882716	24.469252101845786
20	26.043442361751957	23.900228986925356	24.34606048903457	25.710268162288113
21	27.672737675894705	23.90610182923976	23.737884981137796	24.68327551372774
22	26.26559504157197	23.969644791701835	24.44101293804275	25.323747228683445
23	24.416270445120624	23.68414661668164	25.26265534678443	26.6369275914133
24	25.138376165179437	24.820276847108556	24.621716527895593	25.419630459816418
25	25.226157027706346	24.064553162525613	24.76694171010315	25.94234809966489
26	25.02328116299184	25.265571128988306	25.228072461879318	24.48307524614054
27	25.900249606990418	24.25132711073097	24.259856632947415	25.588566649331202
28	24.693287780850543	24.326352581192186	25.354244987143943	25.62611465081333
29	24.768528253739593	24.62541885857226	25.2176998363364	25.388353051351746
30	24.570722221991886	24.85318584495359	25.570213443941718	25.005878489112803
31	25.670522593599514	24.544485313716084	23.979600902677824	25.805391190006578
32	25.099227797169554	24.47303094793717	23.972523628980145	26.45521762591313
33	24.59021539864891	24.04652113264209	24.982215469727798	26.381047998981206
34	25.74681923216125	24.212812160694895	25.063070395387253	24.977298211756594
35	26.568320985328707	23.97467726065634	24.992847102143383	24.464154651871574
36	24.872033490172896	24.940274397160895	24.476508719375264	25.71118339329095
37	26.06286409851651	24.69446081581761	24.350731084647073	24.89194400101881
38	24.79788996471626	24.314567263297743	25.19728273134188	25.69026004064412
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	286.0
1	237.0
2	188.0
3	188.0
4	438.5
5	689.0
6	689.0
7	794.0
8	899.0
9	871.0
10	843.0
11	843.0
12	1147.5
13	1452.0
14	2018.0
15	2584.0
16	2584.0
17	3974.0
18	5364.0
19	5364.0
20	6653.5
21	7943.0
22	9380.5
23	10818.0
24	10818.0
25	13071.5
26	15325.0
27	15325.0
28	19495.0
29	23665.0
30	29222.0
31	34779.0
32	34779.0
33	46017.5
34	57256.0
35	57256.0
36	64047.0
37	70838.0
38	81234.5
39	91631.0
40	91631.0
41	96427.5
42	101224.0
43	116268.5
44	131313.0
45	131313.0
46	132484.5
47	133656.0
48	133656.0
49	144927.0
50	156198.0
51	158801.5
52	161405.0
53	161405.0
54	152873.0
55	144341.0
56	144341.0
57	141369.5
58	138398.0
59	123379.5
60	108361.0
61	108361.0
62	105049.5
63	101738.0
64	84679.5
65	67621.0
66	67621.0
67	56562.0
68	45503.0
69	45503.0
70	36812.5
71	28122.0
72	21872.5
73	15623.0
74	15623.0
75	11778.5
76	7934.0
77	7934.0
78	8044.0
79	8154.0
80	6290.5
81	4427.0
82	4427.0
83	4794.5
84	5162.0
85	5162.0
86	3394.0
87	1626.0
88	1383.5
89	1141.0
90	1141.0
91	805.5
92	470.0
93	421.0
94	372.0
95	372.0
96	317.5
97	263.0
98	263.0
99	529.0
100	795.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12218617904332309
2	0.054785368693685825
3	0.013563080465788167
4	0.0024875518758214104
5	2.3690970245918196E-4
6	2.961371280739774E-4
7	0.0
8	4.738194049183639E-4
9	4.738194049183639E-4
10	5.922742561479548E-4
11	0.0024875518758214104
12	0.0025467793014362057
13	0.015339903234232032
14	0.009594842949596867
15	0.02517165588628808
16	0.011845485122959097
17	0.02191414747747433
18	0.00550815058217598
19	0.007640337904308618
20	0.005981969987094344
21	0.006515016817627502
22	0.0076995653299234135
23	0.009950207503285641
24	0.01510299353177285
25	0.0183605019405866
26	0.020551916688334032
27	0.009061796119063709
28	0.005804287710249958
29	0.01113475601558155
30	0.0028429164295101835
31	0.005745060284635162
32	0.007107291073775458
33	0.00841029443730096
34	0.012674669081566232
35	0.016583679172142738
36	0.01599140491599478
37	0.009594842949596867
38	0.005567378007790775
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1688407.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.58770613012304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.3945636649739	45.52317934193431
2	10.623210340854804	11.597933684901324
3	2.7339150363218496	4.477144517623853
4	1.0691271959194675	2.3344480474629745
5	0.5806632996650205	1.5848538781330852
6	0.324224975376822	1.0619218605549803
7	0.22662244184372157	0.8659559480499179
8	0.16459153930661474	0.7187739663339264
9	0.10701900703841934	0.5257729895895724
>10	0.6001272928082584	5.9939870262181065
>50	0.0681810602005807	2.659281760600652
>100	0.0845239865391548	10.212346768058318
>500	0.015595834547654213	5.867881576007882
>1k	0.007634324603746819	6.576518634531078
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2787	0.165066835188435	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2679	0.1586702732220371	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2640	0.15636040362306008	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2620	0.15517585511076415	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2540	0.15043766106158052	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2508	0.14854238344190707	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2473	0.14646942354538925	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2458	0.1455810121611673	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2298	0.13610462406280002	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2283	0.13521621267857808	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2275	0.13474239327365972	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2069	0.12254154359701186	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2018	0.1195209448906573	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1978	0.11715184786606546	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1951	0.11555270737446599	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1943	0.11507888796954763	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1928	0.11419047658532569	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1899	0.11247288124249663	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1885	0.1116436972838895	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1843	0.10915614540806809	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1830	0.10838618887507574	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1746	0.10341108512343292	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1710	0.10127889780130028	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1699	0.10062739611953753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.922742561479548E-5
2	0.0	0.0	0.0	0.0	5.922742561479548E-5
3	0.0	0.0	0.0	0.0	5.922742561479548E-5
4	0.0	0.0	0.0	0.0	5.922742561479548E-5
5	0.0	0.0	0.0	0.0	5.922742561479548E-5
6	0.0	0.0	0.0	0.0	5.922742561479548E-5
7	0.0	0.0	0.0	5.922742561479548E-5	5.922742561479548E-5
8	0.0	0.0	0.0	5.922742561479548E-5	5.922742561479548E-5
9	0.0	0.0	0.0	5.922742561479548E-5	5.922742561479548E-5
10	0.0	0.0	0.0	5.922742561479548E-5	5.922742561479548E-5
11	0.0	0.0	0.0	5.922742561479548E-5	5.922742561479548E-5
12	5.922742561479548E-5	0.0	0.0	5.922742561479548E-5	3.553645536887729E-4
13	5.922742561479548E-5	0.0	0.0	5.922742561479548E-5	3.553645536887729E-4
14	5.922742561479548E-5	0.0	0.0	5.922742561479548E-5	4.145919793035684E-4
15	5.922742561479548E-5	0.0	0.0	5.922742561479548E-5	5.330468305331594E-4
16	5.922742561479548E-5	0.0	0.0	1.1845485122959097E-4	5.330468305331594E-4
17	5.922742561479548E-5	0.0	0.0	1.7768227684438644E-4	5.330468305331594E-4
18	5.922742561479548E-5	0.0	0.0	1.7768227684438644E-4	5.330468305331594E-4
19	5.922742561479548E-5	0.0	0.0	1.7768227684438644E-4	5.330468305331594E-4
20	5.922742561479548E-5	0.0	0.0	2.3690970245918193E-4	5.922742561479549E-4
21	5.922742561479548E-5	0.0	0.0	2.3690970245918193E-4	5.922742561479549E-4
22	5.922742561479548E-5	0.0	0.0	2.9613712807397745E-4	6.515016817627503E-4
23	5.922742561479548E-5	0.0	0.0	4.145919793035684E-4	7.699565329923413E-4
24	5.922742561479548E-5	0.0	0.0	8.291839586071368E-4	7.699565329923413E-4
25	5.922742561479548E-5	0.0	0.0	9.476388098367277E-4	7.699565329923413E-4
26	5.922742561479548E-5	0.0	0.0	0.0013030033635255006	7.699565329923413E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAA	85	1.0426447E-8	20.731077	1
TACCGTC	470	0.0	17.360691	7
ACCGTCG	465	0.0	17.203299	8
ATACCGT	510	0.0	16.93818	6
ATCGTTT	450	0.0	16.71063	29
CCTATTC	680	0.0	16.469627	3
GTATTAT	120	3.0664523E-8	16.019468	1
CTCGTGA	60	0.0024430098	15.999541	12
CGAGCCG	710	0.0	15.999541	15
GTCCTAT	735	0.0	15.910493	1
CCGTCGT	510	0.0	15.685359	9
AGGCCCG	680	0.0	15.528966	10
GGTATAT	155	1.8553692E-10	15.502711	1
CATCGTT	490	0.0	15.346499	28
TCTAGCG	565	0.0	15.291596	28
GTATCAA	2630	0.0	15.227631	1
CGTCGTA	530	0.0	15.093906	10
CGCTTCG	555	0.0	14.9905615	32
CTAGCGG	585	0.0	14.768807	29
GAGCCGC	770	0.0	14.752824	16
>>END_MODULE