FastQCFastQC Report
Thu 2 Feb 2017
SRR4062766_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062766_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688407
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG28380.16808743389478958No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT28230.16719902251056765No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28180.16690288538249368No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27550.16317155756876156No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA26600.157544952135356No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC25860.15316212263986112No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC25530.15120761759457288No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG24040.14238273117796835No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG24030.14232350375235353No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG23480.1390659953435398No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA23310.13805912910808826No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC21810.12917501526586894No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21100.12496986804721848No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA20870.12360763725807818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20720.12271922587385625No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT20680.12248231617139707No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG20620.1221269516177083No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA18680.11063683104843797No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG18450.10927460025929768No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT18330.10856387115192014No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT18230.10797159689577218No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC18090.10714241293716505No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT17970.1064316838297875No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG17590.10418104165642525No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT17350.10275958344167016No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT17240.1021080817599074No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT17160.10163426235498904No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC16930.10027203156584875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGT400.004482294420.0015744
TCTAGCG5150.019.88270428
CTAGCGG5400.018.96220829
TAGCGGC5650.018.12317330
TACACCG753.231317E-517.0690215
ACGATTA753.2362484E-517.06598732
AGCGGCG6350.016.377331
GTATCAA25000.015.679841
GCGGCGC7100.015.32333332
GAGTAAT1153.34785E-715.3041921
CTTACAC2000.015.2011963
TCTAGAT5450.014.9718822
GTATAGT1205.4211887E-714.6665171
GTCTAGA1757.4578566E-1114.6284231
CATCGTT5300.014.48998928
ATCGTTT5200.014.46096229
ACCGTCG4650.014.4536068
GTATAAA1452.0318112E-814.3446821
TACCGTC4700.014.2998447
TAGCGTA5150.014.2932347
Produced by FastQC (version 0.11.5)