##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062766_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1688407
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.232366958914525	32.0	32.0	32.0	32.0	32.0
2	31.31315257517885	32.0	32.0	32.0	32.0	32.0
3	31.391412734014963	32.0	32.0	32.0	32.0	32.0
4	31.480747236892526	32.0	32.0	32.0	32.0	32.0
5	31.413824984141858	32.0	32.0	32.0	32.0	32.0
6	34.93490076740975	36.0	36.0	36.0	36.0	36.0
7	34.965909878364634	36.0	36.0	36.0	36.0	36.0
8	34.901343692604925	36.0	36.0	36.0	36.0	36.0
9	35.00186447935835	36.0	36.0	36.0	36.0	36.0
10	34.84779084663828	36.0	36.0	36.0	32.0	36.0
11	35.02676724273235	36.0	36.0	36.0	36.0	36.0
12	34.91827918268522	36.0	36.0	36.0	32.0	36.0
13	34.97730168140738	36.0	36.0	36.0	36.0	36.0
14	34.92415395103195	36.0	36.0	36.0	32.0	36.0
15	34.89201063487654	36.0	36.0	36.0	32.0	36.0
16	34.89392545754667	36.0	36.0	36.0	32.0	36.0
17	34.86741881548702	36.0	36.0	36.0	32.0	36.0
18	34.865298473650014	36.0	36.0	36.0	32.0	36.0
19	34.86088129224766	36.0	36.0	36.0	32.0	36.0
20	34.847244769774115	36.0	36.0	36.0	32.0	36.0
21	34.83968024297459	36.0	36.0	36.0	32.0	36.0
22	34.82145714866143	36.0	36.0	36.0	32.0	36.0
23	34.76644316210487	36.0	36.0	36.0	32.0	36.0
24	34.73412394049539	36.0	36.0	36.0	32.0	36.0
25	34.72337238592354	36.0	36.0	36.0	32.0	36.0
26	34.655612657374675	36.0	36.0	36.0	32.0	36.0
27	34.64280235748845	36.0	36.0	36.0	32.0	36.0
28	34.6058284524999	36.0	36.0	36.0	32.0	36.0
29	34.573949882936994	36.0	36.0	36.0	32.0	36.0
30	34.55240946051515	36.0	36.0	36.0	32.0	36.0
31	34.55781573992527	36.0	36.0	36.0	32.0	36.0
32	34.52929418084621	36.0	36.0	36.0	32.0	36.0
33	34.488847179619604	36.0	36.0	36.0	32.0	36.0
34	34.4934082836662	36.0	36.0	36.0	32.0	36.0
35	34.4609475085095	36.0	36.0	36.0	32.0	36.0
36	34.43577940626875	36.0	36.0	36.0	32.0	36.0
37	34.43001183956238	36.0	36.0	36.0	32.0	36.0
38	33.98489345282269	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	13.0
21	38.0
22	133.0
23	433.0
24	1173.0
25	2939.0
26	5882.0
27	11572.0
28	19598.0
29	30940.0
30	46330.0
31	66570.0
32	95690.0
33	153011.0
34	376317.0
35	877767.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64653530737541	18.477472167842937	11.907750356139067	26.968242168642586
2	16.119108792553448	20.514437878093304	36.20485831014578	27.161595019207464
3	17.610386595175214	24.18652611603719	29.130357786955397	29.0727295018322
4	12.15177892955651	15.974444489852244	36.053181616000444	35.8205949645908
5	14.423189683050166	36.61542306767448	33.22778999837717	15.733597250898185
6	34.55916408731075	36.06265317702022	16.182245265652927	13.195937470016098
7	30.256811182184318	30.563906657190238	20.95859985785359	18.220682302771856
8	28.065132681303673	32.83245546441565	19.40837577567463	19.69403607860604
9	27.425271139588837	13.774145801460252	18.466929291960078	40.33365376699083
10	15.986512610815499	26.192673265450207	31.198027472293166	26.62278665144113
11	38.34687335636203	20.675603318786397	21.962142427300858	19.015380897550713
12	24.711472741681188	23.792023463606416	28.232139028501507	23.26436476621089
13	29.466058835340057	18.68862187849257	25.21512881668934	26.63019046947803
14	23.60112224126055	19.834198744733943	24.45891304643963	32.10576596756587
15	25.338795681373032	27.097790994706845	21.74890296000905	25.81451036391107
16	26.369530569347322	25.36219051449088	23.164497659628278	25.103781256533527
17	24.422547407112148	25.769319838166982	24.72105363221072	25.087079122510154
18	25.822742976071527	24.047223210991188	25.69256109457021	24.437472718367076
19	26.01617974812945	24.685813313969916	24.811316228847666	24.486690709052972
20	26.057875855762262	23.824646545530786	24.311436756658793	25.806040842048155
21	27.570738317679517	23.974506131819005	23.74867182419394	24.706083726307533
22	26.196231121998427	24.010442979684402	24.403002356659265	25.390323541657907
23	24.35607828458118	23.538797586359664	25.31728188277213	26.787842246287024
24	25.175387214101814	24.747469064034917	24.658864835315182	25.41827888654809
25	25.207059447857265	24.075339788344497	24.78766930189694	25.9299314619013
26	25.055747814359925	25.15447993285979	25.132684240233544	24.657088012546737
27	26.03688096161762	24.223216586067917	24.12300366322061	25.61689878909385
28	24.73343216416421	24.24036384592104	25.394173324322868	25.632030665591888
29	24.807999493013234	24.522167936996233	25.214773452135653	25.455059117854876
30	24.729937746052936	24.664432213322975	25.573454741658853	25.032175298965235
31	25.608280467920352	24.446771424188597	24.016543404522725	25.928404703368322
32	25.115745196507717	24.430187745016458	24.018320227291166	26.43574683118466
33	24.622203058859622	23.90347824902408	25.060130643855423	26.41418804826088
34	25.717199703625965	24.04982921771824	25.118114293532308	25.11485678512349
35	26.559828287847658	23.93090054708373	24.947776217464153	24.56149494760446
36	24.978515251358232	24.811790048252583	24.452042665068316	25.757652035320866
37	26.141445753304744	24.555927569596665	24.34946076390349	24.9531659131951
38	25.021144228514032	24.044245334647396	25.20320965835191	25.731400778486663
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	141.0
1	120.0
2	99.0
3	99.0
4	363.0
5	627.0
6	627.0
7	733.5
8	840.0
9	818.5
10	797.0
11	797.0
12	1114.5
13	1432.0
14	1992.0
15	2552.0
16	2552.0
17	4052.5
18	5553.0
19	5553.0
20	6739.5
21	7926.0
22	9233.0
23	10540.0
24	10540.0
25	12845.0
26	15150.0
27	15150.0
28	19347.0
29	23544.0
30	29130.5
31	34717.0
32	34717.0
33	46528.0
34	58339.0
35	58339.0
36	64591.0
37	70843.0
38	81364.0
39	91885.0
40	91885.0
41	96466.0
42	101047.0
43	116171.5
44	131296.0
45	131296.0
46	132405.0
47	133514.0
48	133514.0
49	145531.5
50	157549.0
51	159116.0
52	160683.0
53	160683.0
54	153260.5
55	145838.0
56	145838.0
57	141971.0
58	138104.0
59	123386.5
60	108669.0
61	108669.0
62	105548.5
63	102428.0
64	84809.0
65	67190.0
66	67190.0
67	56586.0
68	45982.0
69	45982.0
70	37219.5
71	28457.0
72	22193.0
73	15929.0
74	15929.0
75	11775.5
76	7622.0
77	7622.0
78	7824.5
79	8027.0
80	6292.5
81	4558.0
82	4558.0
83	4497.5
84	4437.0
85	4437.0
86	2770.0
87	1103.0
88	940.0
89	777.0
90	777.0
91	449.5
92	122.0
93	85.5
94	49.0
95	49.0
96	35.0
97	21.0
98	21.0
99	20.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009594842949596867
2	5.922742561479549E-5
3	0.0
4	2.3690970245918196E-4
5	5.922742561479549E-5
6	5.922742561479549E-5
7	4.1459197930356843E-4
8	0.0022506421733622284
9	0.0041459197930356845
10	8.884113842219322E-4
11	0.004678966623568843
12	2.961371280739774E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	5.922742561479549E-5
22	0.0
23	1.7768227684438647E-4
24	0.0
25	1.7768227684438647E-4
26	0.0
27	1.1845485122959098E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.7768227684438647E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1688407.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.90991306638764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.45808618390113	43.628501716109085
2	11.24265291281265	11.896955765049743
3	2.7975180876012935	4.440493164498943
4	1.1505825321449246	2.435088870059685
5	0.6135310489904112	1.6230937232806135
6	0.3655398802157278	1.1604409970707146
7	0.24597543290397034	0.9110177139991293
8	0.1638151682337681	0.6933957048163468
9	0.12606252912757018	0.6002961711361828
>10	0.6492840630046985	6.216443798657753
>50	0.07301432965676884	2.72907349355603
>100	0.08863103405540666	10.340022526014602
>500	0.016083875650228036	5.765292747076199
>1k	0.009222921701529364	7.559883608674996
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2838	0.16808743389478958	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2823	0.16719902251056765	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2818	0.16690288538249368	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2755	0.16317155756876156	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2660	0.157544952135356	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2586	0.15316212263986112	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2553	0.15120761759457288	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2404	0.14238273117796835	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2403	0.14232350375235353	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2348	0.1390659953435398	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2331	0.13805912910808826	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2181	0.12917501526586894	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2110	0.12496986804721848	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2087	0.12360763725807818	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2072	0.12271922587385625	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2068	0.12248231617139707	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2062	0.1221269516177083	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1868	0.11063683104843797	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1845	0.10927460025929768	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1833	0.10856387115192014	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1823	0.10797159689577218	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1809	0.10714241293716505	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1797	0.1064316838297875	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1759	0.10418104165642525	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1735	0.10275958344167016	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1724	0.1021080817599074	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1716	0.10163426235498904	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1693	0.10027203156584875	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.922742561479548E-5
7	0.0	0.0	0.0	0.0	5.922742561479548E-5
8	0.0	0.0	0.0	0.0	5.922742561479548E-5
9	0.0	0.0	0.0	0.0	5.922742561479548E-5
10	0.0	0.0	0.0	0.0	5.922742561479548E-5
11	0.0	0.0	0.0	0.0	5.922742561479548E-5
12	0.0	0.0	0.0	0.0	5.922742561479548E-5
13	0.0	0.0	0.0	0.0	1.1845485122959097E-4
14	0.0	0.0	0.0	0.0	1.7768227684438644E-4
15	0.0	0.0	0.0	0.0	2.3690970245918193E-4
16	0.0	0.0	0.0	5.922742561479548E-5	2.3690970245918193E-4
17	0.0	0.0	0.0	1.1845485122959097E-4	2.3690970245918193E-4
18	0.0	0.0	0.0	1.1845485122959097E-4	2.9613712807397745E-4
19	0.0	0.0	0.0	1.1845485122959097E-4	2.9613712807397745E-4
20	0.0	0.0	0.0	1.7768227684438644E-4	4.145919793035684E-4
21	0.0	0.0	0.0	1.7768227684438644E-4	4.145919793035684E-4
22	0.0	0.0	0.0	2.3690970245918193E-4	4.145919793035684E-4
23	0.0	0.0	0.0	3.553645536887729E-4	4.7381940491836387E-4
24	0.0	0.0	0.0	6.515016817627503E-4	4.7381940491836387E-4
25	5.922742561479548E-5	0.0	0.0	7.699565329923413E-4	4.7381940491836387E-4
26	5.922742561479548E-5	0.0	0.0	0.0011845485122959098	4.7381940491836387E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGGT	40	0.0044822944	20.001574	4
TCTAGCG	515	0.0	19.882704	28
CTAGCGG	540	0.0	18.962208	29
TAGCGGC	565	0.0	18.123173	30
TACACCG	75	3.231317E-5	17.069021	5
ACGATTA	75	3.2362484E-5	17.065987	32
AGCGGCG	635	0.0	16.3773	31
GTATCAA	2500	0.0	15.67984	1
GCGGCGC	710	0.0	15.323333	32
GAGTAAT	115	3.34785E-7	15.304192	1
CTTACAC	200	0.0	15.201196	3
TCTAGAT	545	0.0	14.971882	2
GTATAGT	120	5.4211887E-7	14.666517	1
GTCTAGA	175	7.4578566E-11	14.628423	1
CATCGTT	530	0.0	14.489989	28
ATCGTTT	520	0.0	14.460962	29
ACCGTCG	465	0.0	14.453606	8
GTATAAA	145	2.0318112E-8	14.344682	1
TACCGTC	470	0.0	14.299844	7
TAGCGTA	515	0.0	14.293234	7
>>END_MODULE