FastQCFastQC Report
Thu 2 Feb 2017
SRR4062765_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062765_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1845718
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29370.1591250667761814No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT24170.13095174885870972No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23860.12927218567516816No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23590.12780934032176094No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21990.11914062711638505No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21310.1154564240041003No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21140.11453537322602911No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21100.11431865539589471No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20850.11296416895755473No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20820.11280163058495395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20310.11003847825074035No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19600.1061917367658548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG1750.019.1980674
GTATTAG5200.016.6344411
CGTACAC908.581403E-616.0009883
ATCGTTT4050.015.80173429
CATCGTT4050.015.80130528
ATACCGT4800.015.6646636
CGGCATC4400.015.63563525
TACCGTC4750.015.4927567
ACCGTCG4800.015.3313738
TCTAGCG4100.015.2183928
GTCCTAT6650.015.1752811
TATGCCG859.4474075E-515.0568985
GTTTTCG6600.015.02919528
GCATCGT4550.014.76774227
CCGTCGT5100.014.7448119
ATATTAC1205.351503E-714.6834881
CAAGACG6250.014.5905294
CTTATAC2100.014.4770863
CGTCGTA5100.014.43109210
TTTTCGG6800.014.35227329
Produced by FastQC (version 0.11.5)