##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062765_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1845718
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26385612536693	32.0	32.0	32.0	32.0	32.0
2	30.93789354603466	32.0	32.0	32.0	32.0	32.0
3	30.98637874258148	32.0	32.0	32.0	32.0	32.0
4	31.013709569934303	32.0	32.0	32.0	32.0	32.0
5	30.985444146939024	32.0	32.0	32.0	32.0	32.0
6	34.55547001221205	36.0	36.0	36.0	32.0	36.0
7	34.526887639390196	36.0	36.0	36.0	32.0	36.0
8	34.51165887746666	36.0	36.0	36.0	32.0	36.0
9	34.624023279829316	36.0	36.0	36.0	32.0	36.0
10	34.356945643917435	36.0	36.0	36.0	32.0	36.0
11	34.613952944057544	36.0	36.0	36.0	32.0	36.0
12	34.44475374894756	36.0	36.0	36.0	32.0	36.0
13	34.52723005356181	36.0	36.0	36.0	32.0	36.0
14	34.41367370313341	36.0	36.0	36.0	32.0	36.0
15	34.36794949174251	36.0	36.0	36.0	32.0	36.0
16	34.39452343207359	36.0	36.0	36.0	32.0	36.0
17	34.3102467440855	36.0	36.0	36.0	32.0	36.0
18	34.33337866347947	36.0	36.0	36.0	32.0	36.0
19	34.31271841093818	36.0	36.0	36.0	32.0	36.0
20	34.299088484806454	36.0	36.0	36.0	32.0	36.0
21	34.27391562524719	36.0	36.0	36.0	32.0	36.0
22	34.25212139665973	36.0	36.0	36.0	32.0	36.0
23	34.20711723025944	36.0	36.0	36.0	32.0	36.0
24	34.19516578372211	36.0	36.0	36.0	32.0	36.0
25	34.15913265190024	36.0	36.0	36.0	32.0	36.0
26	34.12132080848754	36.0	36.0	36.0	32.0	36.0
27	34.1110234607887	36.0	36.0	36.0	32.0	36.0
28	34.093908711948416	36.0	36.0	36.0	32.0	36.0
29	34.06730063855909	36.0	36.0	36.0	32.0	36.0
30	34.04310030026255	36.0	36.0	36.0	32.0	36.0
31	34.05299021844073	36.0	36.0	36.0	32.0	36.0
32	34.02526821540452	36.0	36.0	36.0	32.0	36.0
33	33.98213703285117	36.0	36.0	36.0	32.0	36.0
34	33.99179831371856	36.0	36.0	36.0	32.0	36.0
35	33.953044289539356	36.0	36.0	36.0	32.0	36.0
36	33.916784145790416	36.0	36.0	36.0	32.0	36.0
37	33.91599908545076	36.0	36.0	36.0	32.0	36.0
38	33.20768882353642	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	6.0
10	8.0
11	28.0
12	8.0
13	13.0
14	181.0
15	406.0
16	596.0
17	769.0
18	939.0
19	1219.0
20	1656.0
21	2229.0
22	3393.0
23	4785.0
24	7131.0
25	10710.0
26	15758.0
27	22841.0
28	32691.0
29	45647.0
30	62298.0
31	85434.0
32	120401.0
33	190791.0
34	429865.0
35	805910.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.519146699416375	16.95551395542228	11.32609736926471	26.199241975896637
2	16.991685735210968	19.240283173981563	37.17555691804619	26.592474172761282
3	18.89584281596116	23.189630881938577	28.37012592929753	29.544400372802738
4	12.649574594546856	14.875780957504967	35.67061161617229	36.80403283177589
5	14.770714623562819	36.231906043897396	33.662817566978184	15.334561765561602
6	35.150462097594755	35.12868190846469	16.35610934305098	13.364746650889575
7	30.881532281746182	30.07880943892837	20.612737157030487	18.426921122294956
8	28.47779857670334	32.81871794941256	19.10739198630341	19.596091487580694
9	27.436019409269896	14.082394041120086	18.32221208463274	40.159374464977276
10	15.857178228684523	26.2656220116562	31.521856323274527	26.35534343638475
11	38.13485948840646	20.781401366647813	21.839979108016326	19.243760036929398
12	24.739254579637745	23.510865972790366	28.470853402829327	23.279026044742558
13	29.586458363249267	18.85449015388667	25.092917766895752	26.46613371596831
14	23.642123879612	19.42330970553207	24.684026472095823	32.250539942760106
15	25.369308497983766	26.910814822522195	21.980541632593116	25.73933504690092
16	26.279998851277874	25.411088094524658	23.350486500076673	24.9584265541208
17	24.389857961827275	25.728791681139988	24.895507570654658	24.98584278637808
18	25.519080590306014	24.321487804362654	25.75509896679651	24.40433263853482
19	25.817183460782182	24.85271543804458	25.007409606860076	24.322691494313162
20	25.937388586729774	23.977436137507137	24.648381073750304	25.43679420201278
21	27.129812196270365	23.999822279728455	23.99976809671884	24.870597427282338
22	25.937695737076748	24.091563230796904	24.635834216520696	25.334906815605652
23	24.379301245542635	23.87365005708353	25.359013969345572	26.38803472802826
24	25.02577946648561	24.777711368595707	24.799982226663147	25.396526938255537
25	25.185865145354132	24.170976426013752	24.90067323281804	25.74248519581408
26	24.870486537102625	25.077111990670698	25.2857966541091	24.766604818117578
27	25.672540286311868	24.523554081732176	24.34452788885638	25.45937774309958
28	24.733083841338697	24.417525412910695	25.324214070416563	25.52517667533405
29	24.724820403116297	24.835791716381596	25.26873155670862	25.170656323793484
30	24.73940315343992	24.84706071030148	25.467215917064127	24.94632021919447
31	25.369675526453385	24.67673202012348	24.29181758827051	25.66177486515262
32	25.00478164541702	24.63492882256655	24.33090828506084	26.029381246955595
33	24.57215091930998	24.228842161956557	25.070696354349252	26.12831056438421
34	25.51837406162726	24.378780177753022	25.13944866435977	24.96339709625995
35	26.051444282963065	24.215812820321077	25.100817485940823	24.631925410775036
36	24.76267370377495	24.98028941372182	24.748259914221695	25.50877696828153
37	25.869936972240044	24.71585490610858	24.558014582213513	24.856193539437864
38	24.84544304881128	24.610234731507077	25.06103712726173	25.483285092419912
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	79.0
2	129.0
3	129.0
4	411.0
5	693.0
6	693.0
7	835.5
8	978.0
9	944.5
10	911.0
11	911.0
12	1169.5
13	1428.0
14	2118.0
15	2808.0
16	2808.0
17	4269.5
18	5731.0
19	5731.0
20	7049.0
21	8367.0
22	9870.5
23	11374.0
24	11374.0
25	13903.5
26	16433.0
27	16433.0
28	20848.5
29	25264.0
30	31187.0
31	37110.0
32	37110.0
33	48226.5
34	59343.0
35	59343.0
36	67411.0
37	75479.0
38	86738.5
39	97998.0
40	97998.0
41	105546.0
42	113094.0
43	128553.0
44	144012.0
45	144012.0
46	149380.0
47	154748.0
48	154748.0
49	164746.5
50	174745.0
51	177219.0
52	179693.0
53	179693.0
54	171118.0
55	162543.0
56	162543.0
57	157485.5
58	152428.0
59	136727.0
60	121026.0
61	121026.0
62	113848.5
63	106671.0
64	89007.0
65	71343.0
66	71343.0
67	59523.0
68	47703.0
69	47703.0
70	38159.0
71	28615.0
72	22551.5
73	16488.0
74	16488.0
75	12446.0
76	8404.0
77	8404.0
78	8039.5
79	7675.0
80	6005.0
81	4335.0
82	4335.0
83	4347.5
84	4360.0
85	4360.0
86	2843.5
87	1327.0
88	1126.0
89	925.0
90	925.0
91	647.0
92	369.0
93	314.0
94	259.0
95	259.0
96	220.0
97	181.0
98	181.0
99	440.0
100	699.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12141616433279624
2	0.057267686613014554
3	0.012894710892996656
4	0.0036300236547511596
5	2.7089728766799693E-4
6	2.1671783013439756E-4
7	0.0
8	1.625383726007982E-4
9	1.0835891506719878E-4
10	2.7089728766799693E-4
11	0.0018421015561423792
12	0.002600613961612771
13	0.014574274076538236
14	0.008235277545107108
15	0.02410985860245173
16	0.010239917473850285
17	0.019992219829898177
18	0.00536376629582634
19	0.007639303512237514
20	0.006068099243763132
21	0.0065557143615655265
22	0.0070433294793679204
23	0.009589763983447092
24	0.013924120586135045
25	0.015928760514878224
26	0.018962810136759788
27	0.009643943440980692
28	0.005472125210893538
29	0.01061917367658548
30	0.002763152334213569
31	0.006122278701296731
32	0.006447355446498328
33	0.008072739172506308
34	0.012786351977929456
35	0.016470555090214216
36	0.014249197331336639
37	0.00986066127111509
38	0.006501534904031927
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1845718.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.99389768939311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6158869358558	44.33381838290137
2	11.870416173520784	13.056009051542386
3	3.357909806873083	5.539936451081644
4	1.379127645234088	3.0337441849046827
5	0.6998194498138948	1.924289961205636
6	0.43686405566054504	1.4414914308701614
7	0.2876515178721584	1.1073354700842082
8	0.21406878828614476	0.9417981633200481
9	0.15842542251730368	0.7841188329583927
>10	0.8228099165826838	8.100355147919556
>50	0.06928790245452697	2.652099492967326
>100	0.07099871796704382	8.409173441207022
>500	0.011584846635225493	4.355557649396992
>1k	0.005148820726766886	4.320272339640413
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2937	0.1591250667761814	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2417	0.13095174885870972	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2386	0.12927218567516816	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2359	0.12780934032176094	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2199	0.11914062711638505	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2131	0.1154564240041003	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2114	0.11453537322602911	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2110	0.11431865539589471	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2085	0.11296416895755473	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2082	0.11280163058495395	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2031	0.11003847825074035	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1960	0.1061917367658548	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.417945753359939E-5
2	0.0	0.0	0.0	0.0	5.417945753359939E-5
3	0.0	0.0	0.0	0.0	5.417945753359939E-5
4	0.0	0.0	0.0	0.0	5.417945753359939E-5
5	0.0	0.0	0.0	5.417945753359939E-5	5.417945753359939E-5
6	0.0	0.0	0.0	5.417945753359939E-5	5.417945753359939E-5
7	0.0	0.0	0.0	5.417945753359939E-5	5.417945753359939E-5
8	0.0	0.0	0.0	5.417945753359939E-5	5.417945753359939E-5
9	0.0	0.0	0.0	5.417945753359939E-5	5.417945753359939E-5
10	0.0	0.0	0.0	5.417945753359939E-5	5.417945753359939E-5
11	0.0	0.0	0.0	5.417945753359939E-5	5.417945753359939E-5
12	0.0	0.0	0.0	5.417945753359939E-5	2.1671783013439756E-4
13	0.0	0.0	0.0	5.417945753359939E-5	2.7089728766799693E-4
14	0.0	0.0	0.0	5.417945753359939E-5	2.7089728766799693E-4
15	0.0	0.0	0.0	5.417945753359939E-5	4.334356602687951E-4
16	0.0	0.0	0.0	5.417945753359939E-5	5.417945753359939E-4
17	0.0	0.0	0.0	5.417945753359939E-5	5.417945753359939E-4
18	0.0	0.0	0.0	5.417945753359939E-5	5.417945753359939E-4
19	0.0	0.0	0.0	5.417945753359939E-5	5.417945753359939E-4
20	0.0	0.0	0.0	1.0835891506719878E-4	5.959740328695933E-4
21	0.0	0.0	0.0	2.7089728766799693E-4	7.043329479367921E-4
22	0.0	0.0	0.0	4.334356602687951E-4	7.043329479367921E-4
23	0.0	0.0	0.0	5.959740328695933E-4	7.043329479367921E-4
24	0.0	0.0	0.0	9.210507780711896E-4	7.585124054703915E-4
25	0.0	0.0	0.0	9.75230235604789E-4	7.585124054703915E-4
26	0.0	0.0	0.0	0.0011377686082055872	7.585124054703915E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	175	0.0	19.198067	4
GTATTAG	520	0.0	16.634441	1
CGTACAC	90	8.581403E-6	16.000988	3
ATCGTTT	405	0.0	15.801734	29
CATCGTT	405	0.0	15.801305	28
ATACCGT	480	0.0	15.664663	6
CGGCATC	440	0.0	15.635635	25
TACCGTC	475	0.0	15.492756	7
ACCGTCG	480	0.0	15.331373	8
TCTAGCG	410	0.0	15.21839	28
GTCCTAT	665	0.0	15.175281	1
TATGCCG	85	9.4474075E-5	15.056898	5
GTTTTCG	660	0.0	15.029195	28
GCATCGT	455	0.0	14.767742	27
CCGTCGT	510	0.0	14.744811	9
ATATTAC	120	5.351503E-7	14.683488	1
CAAGACG	625	0.0	14.590529	4
CTTATAC	210	0.0	14.477086	3
CGTCGTA	510	0.0	14.431092	10
TTTTCGG	680	0.0	14.352273	29
>>END_MODULE