FastQCFastQC Report
Thu 2 Feb 2017
SRR4062765_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062765_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1845718
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30240.16383867958160456No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26550.14384645975170637No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23540.12753844303409295No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23110.12520872636014818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22890.12401677829440902No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22640.12266229185606901No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC22470.12174124107799783No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22370.12119944650266185No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22190.12022421626705704No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21010.11383104027809232No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20350.11025519608087476No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG18920.10250753365357004No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT18550.10050289372482686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG507.193114E-419.2020175
TCTAGCG4400.018.5448928
CTAGCGG4700.018.04200229
CGCTTCG4450.017.9769832
CGTTTAT4750.017.51525731
CATCGTT4700.017.36117228
TAGCGGC5000.017.27947430
ATACCGT4650.017.2061066
ACCGTCG4550.016.8808948
TAAACGC4850.016.8242328
ATCGTTT4950.016.48434429
TAACGAA2350.016.33992813
TACCGTC4800.016.3350497
CGTCGTA4550.016.17620810
CCGTCGT4750.016.1701189
CAAGACG6950.016.1163644
AACGCTT5000.015.99951330
GTAAACG5200.015.99951227
ATAACGA2650.015.69763612
TATACTG1950.015.591385
Produced by FastQC (version 0.11.5)