##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062765_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1845718
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.159965390162526	32.0	32.0	32.0	32.0	32.0
2	31.176033391883266	32.0	32.0	32.0	32.0	32.0
3	31.30176765898149	32.0	32.0	32.0	32.0	32.0
4	31.420188782901832	32.0	32.0	32.0	32.0	32.0
5	31.300590339369286	32.0	32.0	32.0	32.0	32.0
6	34.828745236271196	36.0	36.0	36.0	32.0	36.0
7	34.861051905003904	36.0	36.0	36.0	32.0	36.0
8	34.78759810545273	36.0	36.0	36.0	32.0	36.0
9	34.943436104540346	36.0	36.0	36.0	32.0	36.0
10	34.74172219158073	36.0	36.0	36.0	32.0	36.0
11	34.95657516478682	36.0	36.0	36.0	36.0	36.0
12	34.835229433748815	36.0	36.0	36.0	32.0	36.0
13	34.911617050925436	36.0	36.0	36.0	32.0	36.0
14	34.84710502904561	36.0	36.0	36.0	32.0	36.0
15	34.809428092482165	36.0	36.0	36.0	32.0	36.0
16	34.82077597986258	36.0	36.0	36.0	32.0	36.0
17	34.78768804335223	36.0	36.0	36.0	32.0	36.0
18	34.79040297596924	36.0	36.0	36.0	32.0	36.0
19	34.77966514928066	36.0	36.0	36.0	32.0	36.0
20	34.77401477365448	36.0	36.0	36.0	32.0	36.0
21	34.76783994087938	36.0	36.0	36.0	32.0	36.0
22	34.74270229796751	36.0	36.0	36.0	32.0	36.0
23	34.68510574204727	36.0	36.0	36.0	32.0	36.0
24	34.66065346927321	36.0	36.0	36.0	32.0	36.0
25	34.6379880350086	36.0	36.0	36.0	32.0	36.0
26	34.565057609017195	36.0	36.0	36.0	32.0	36.0
27	34.558674727125165	36.0	36.0	36.0	32.0	36.0
28	34.52638864658631	36.0	36.0	36.0	32.0	36.0
29	34.47796196385363	36.0	36.0	36.0	32.0	36.0
30	34.46647808603481	36.0	36.0	36.0	32.0	36.0
31	34.46387259592202	36.0	36.0	36.0	32.0	36.0
32	34.43492559535097	36.0	36.0	36.0	32.0	36.0
33	34.396431632567925	36.0	36.0	36.0	32.0	36.0
34	34.39667544012682	36.0	36.0	36.0	32.0	36.0
35	34.36446683621225	36.0	36.0	36.0	32.0	36.0
36	34.334522391828	36.0	36.0	36.0	32.0	36.0
37	34.33191581812606	36.0	36.0	36.0	32.0	36.0
38	33.86055182861087	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	32.0
22	114.0
23	454.0
24	1193.0
25	3000.0
26	6218.0
27	12375.0
28	21634.0
29	34894.0
30	53947.0
31	79354.0
32	116463.0
33	190920.0
34	455817.0
35	869297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65399993172837	18.30936692152935	12.042519136374743	26.994114010367536
2	15.947073171524579	20.197722512323118	36.387248756310555	27.46795555984175
3	17.92608621685436	23.884201161824286	28.753904984401736	29.435807636919613
4	12.134532073190025	15.735356902145291	35.75517576918659	36.37493525547809
5	14.402803896805416	36.70329741775147	33.189703513593905	15.704195171849205
6	35.23882898433885	35.561793430618174	15.955505565867734	13.243872019175235
7	30.632856389759826	30.247584806072027	20.558581489734852	18.5609773144333
8	28.42816739629838	33.079136144943874	18.869305621776256	19.623390836981493
9	27.361358087702246	13.7683444459254	18.110436734079087	40.75986073229327
10	15.698579727388278	26.47924746248311	31.45605303564714	26.366119774481472
11	38.37363847230402	20.7139679751456	21.654026892728712	19.258366659821665
12	24.510406270960722	23.58008878948743	28.391397583807677	23.51810735574417
13	29.633610822906025	18.817043801453636	25.056251913215206	26.493093462425133
14	23.532793200261363	19.320882171599347	24.62570121762913	32.52062341051016
15	25.410436480545783	27.009055554532164	21.704832482535252	25.8756754823868
16	26.245937895171416	25.49874899632555	23.13191939397026	25.12339371453277
17	24.47491978731312	25.798090499198683	24.739911514109956	24.98707819937824
18	25.60862493620369	24.34873583071737	25.553307710061883	24.489331523017057
19	25.929150606972463	24.811861833714577	24.81614201085973	24.44284554845323
20	25.965342484604907	23.919363629763595	24.610043354401917	25.505250531229578
21	27.155384227792485	24.024673365064487	23.97650774902951	24.843434658113523
22	25.976070017196562	24.041809203789526	24.606955125322504	25.37516565369141
23	24.423408355668826	23.735274692707495	25.26822909470954	26.57308785691414
24	25.07625758647854	24.684377570138018	24.71721032140338	25.522154521980063
25	25.214361023731684	24.194649453491813	24.885708434332873	25.705281088443634
26	25.088664682253736	24.966002390397666	25.119546973047886	24.82578595430071
27	25.812164933372706	24.35500605456422	24.25759123158234	25.575237780480737
28	24.8031931205092	24.277164767315483	25.381829726968043	25.537812385207275
29	24.909764113477788	24.606413330747166	25.16494935846104	25.318873197314
30	24.84967909507303	24.685081903085955	25.491055513355775	24.97418348848524
31	25.504600377739177	24.477141145071997	24.323542382964245	25.694716094224578
32	25.16733325459252	24.482342372995227	24.30029939568233	26.050024976729922
33	24.652303331278127	24.06624413913718	25.12653612306972	26.15491640651497
34	25.492030743591382	24.243465144729583	25.145173856461277	25.119330255217754
35	26.18558197947899	24.057629605389337	25.018502284747722	24.738286130383948
36	24.92244210654065	24.853417477642846	24.65192407507539	25.57221634074111
37	26.074730809365242	24.536792727816493	24.501955336622387	24.886521126195877
38	25.038467456531777	24.180372278292	25.082190326139013	25.69896993903721
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	40.0
2	61.0
3	61.0
4	267.0
5	473.0
6	473.0
7	614.5
8	756.0
9	797.0
10	838.0
11	838.0
12	1177.5
13	1517.0
14	2160.0
15	2803.0
16	2803.0
17	4322.0
18	5841.0
19	5841.0
20	7135.0
21	8429.0
22	9942.0
23	11455.0
24	11455.0
25	13763.5
26	16072.0
27	16072.0
28	20628.0
29	25184.0
30	31084.0
31	36984.0
32	36984.0
33	48265.0
34	59546.0
35	59546.0
36	67360.5
37	75175.0
38	86182.0
39	97189.0
40	97189.0
41	104420.5
42	111652.0
43	127695.5
44	143739.0
45	143739.0
46	149351.0
47	154963.0
48	154963.0
49	164943.0
50	174923.0
51	176552.0
52	178181.0
53	178181.0
54	170589.0
55	162997.0
56	162997.0
57	158504.5
58	154012.0
59	138191.0
60	122370.0
61	122370.0
62	114978.0
63	107586.0
64	89621.5
65	71657.0
66	71657.0
67	60125.0
68	48593.0
69	48593.0
70	39067.5
71	29542.0
72	23122.5
73	16703.0
74	16703.0
75	12623.5
76	8544.0
77	8544.0
78	8153.5
79	7763.0
80	6078.5
81	4394.0
82	4394.0
83	4042.0
84	3690.0
85	3690.0
86	2404.0
87	1118.0
88	914.0
89	710.0
90	710.0
91	422.5
92	135.0
93	93.0
94	51.0
95	51.0
96	39.5
97	28.0
98	28.0
99	26.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008072739172506308
2	0.0
3	0.0
4	1.0835891506719878E-4
5	5.417945753359939E-5
6	2.1671783013439756E-4
7	3.792562027351957E-4
8	0.002058819386276777
9	0.003467485282150361
10	8.668713205375902E-4
11	0.004821971720490346
12	2.1671783013439756E-4
13	1.625383726007982E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.625383726007982E-4
22	0.0
23	5.417945753359939E-5
24	0.0
25	0.0
26	0.0
27	1.625383726007982E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.0835891506719878E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1845718.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.61493630559937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.555111224912	42.6534222110853
2	12.563767648190543	13.47211204432172
3	3.5143486631526355	5.652647391917904
4	1.4412808759465419	3.090967294494092
5	0.7713394794886644	2.067765853138946
6	0.4480218513072649	1.441239781281343
7	0.3089591094168056	1.1595376080691717
8	0.2108587773492893	0.9044143933646963
9	0.15368524393406996	0.7415842108172095
>10	0.8682675197946266	8.36273703190091
>50	0.072034338426876	2.7059857789680097
>100	0.07544718628795624	8.860862603600491
>500	0.011285946258991163	4.261724285394464
>1k	0.0055921355337343595	4.624999511645695
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3024	0.16383867958160456	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2655	0.14384645975170637	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2354	0.12753844303409295	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2311	0.12520872636014818	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2289	0.12401677829440902	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2264	0.12266229185606901	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2247	0.12174124107799783	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2237	0.12119944650266185	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2219	0.12022421626705704	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2101	0.11383104027809232	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2035	0.11025519608087476	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1892	0.10250753365357004	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1855	0.10050289372482686	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.417945753359939E-5	0.0
6	0.0	0.0	0.0	5.417945753359939E-5	0.0
7	0.0	0.0	0.0	5.417945753359939E-5	0.0
8	0.0	0.0	0.0	5.417945753359939E-5	0.0
9	0.0	0.0	0.0	5.417945753359939E-5	0.0
10	0.0	0.0	0.0	5.417945753359939E-5	5.417945753359939E-5
11	0.0	0.0	0.0	5.417945753359939E-5	5.417945753359939E-5
12	0.0	0.0	0.0	5.417945753359939E-5	2.7089728766799693E-4
13	0.0	0.0	0.0	5.417945753359939E-5	2.7089728766799693E-4
14	0.0	0.0	0.0	5.417945753359939E-5	2.7089728766799693E-4
15	0.0	0.0	0.0	5.417945753359939E-5	2.7089728766799693E-4
16	0.0	0.0	0.0	5.417945753359939E-5	2.7089728766799693E-4
17	0.0	0.0	0.0	1.0835891506719878E-4	2.7089728766799693E-4
18	5.417945753359939E-5	0.0	0.0	1.0835891506719878E-4	2.7089728766799693E-4
19	5.417945753359939E-5	0.0	0.0	1.0835891506719878E-4	3.2507674520159633E-4
20	5.417945753359939E-5	0.0	0.0	1.0835891506719878E-4	4.334356602687951E-4
21	5.417945753359939E-5	0.0	0.0	2.7089728766799693E-4	4.334356602687951E-4
22	5.417945753359939E-5	0.0	0.0	4.334356602687951E-4	4.334356602687951E-4
23	5.417945753359939E-5	0.0	0.0	5.417945753359939E-4	4.334356602687951E-4
24	5.417945753359939E-5	0.0	0.0	8.668713205375902E-4	4.334356602687951E-4
25	5.417945753359939E-5	0.0	0.0	8.668713205375902E-4	4.334356602687951E-4
26	5.417945753359939E-5	0.0	0.0	0.0011377686082055872	4.876151178023945E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGCCG	50	7.193114E-4	19.202017	5
TCTAGCG	440	0.0	18.54489	28
CTAGCGG	470	0.0	18.042002	29
CGCTTCG	445	0.0	17.97698	32
CGTTTAT	475	0.0	17.515257	31
CATCGTT	470	0.0	17.361172	28
TAGCGGC	500	0.0	17.279474	30
ATACCGT	465	0.0	17.206106	6
ACCGTCG	455	0.0	16.880894	8
TAAACGC	485	0.0	16.82423	28
ATCGTTT	495	0.0	16.484344	29
TAACGAA	235	0.0	16.339928	13
TACCGTC	480	0.0	16.335049	7
CGTCGTA	455	0.0	16.176208	10
CCGTCGT	475	0.0	16.170118	9
CAAGACG	695	0.0	16.116364	4
AACGCTT	500	0.0	15.999513	30
GTAAACG	520	0.0	15.999512	27
ATAACGA	265	0.0	15.697636	12
TATACTG	195	0.0	15.59138	5
>>END_MODULE