FastQCFastQC Report
Thu 2 Feb 2017
SRR4062764_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062764_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7417462
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT78720.10612794511114448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73500.020.5248051
GGTATCA27650.019.4028321
TCTAGCG13000.015.99892228
CTAGCGG13250.015.81833529
TAGCGGC14800.014.37789330
CGTATAC1452.0390871E-814.3427963
ACCGTCG12200.014.2939268
ATACCGT14000.014.2826346
TATCAAC107150.013.9507532
TCAACGC105650.013.8841814
TAAACGC11150.013.77485728
TACCGTC12700.013.7311737
AACGCAG108800.013.5853046
CAACGCA108750.013.4884015
ATCGTTT10250.013.42393829
ATCAACG109600.013.414163
CGTCGTA12750.013.3022310
CATCGTT10700.013.0084728
GTCCTAT17100.012.8303931
TAAACCG1751.731496E-812.7970675
Produced by FastQC (version 0.11.5)