FastQCFastQC Report
Thu 2 Feb 2017
SRR4062764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7417462
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT74320.10019599695960694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72000.021.1117781
GGTATCA24150.020.207681
TCTAGCG13150.015.2085828
CTAGCGG13400.015.04423629
AAACGCT11850.014.04169229
TAAACGC11650.014.00808128
TATCAAC108050.013.9934912
TCAACGC106350.013.9016164
TACCGTC12900.013.76899057
ACCGTCG12750.013.6798788
CGTCGTA13000.013.53943710
ATACCGT13950.013.5355726
CAACGCA110050.013.4498565
TTTTCGG15300.013.38514129
ATCAACG111300.013.3405833
AACGCAG111600.013.2918176
TAGCGTA9900.013.2540167
AACCGCG8950.013.2305377
TAGCGGC15650.013.085793530
ATCGTTT10400.012.92261429
Produced by FastQC (version 0.11.5)