##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062764_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7417462
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.195017918527927	32.0	32.0	32.0	32.0	32.0
2	31.22610860696017	32.0	32.0	32.0	32.0	32.0
3	31.342521471630054	32.0	32.0	32.0	32.0	32.0
4	31.45088818790039	32.0	32.0	32.0	32.0	32.0
5	31.359186605876783	32.0	32.0	32.0	32.0	32.0
6	34.89107055755729	36.0	36.0	36.0	36.0	36.0
7	34.92631050890453	36.0	36.0	36.0	36.0	36.0
8	34.84896545476067	36.0	36.0	36.0	32.0	36.0
9	34.984438747377474	36.0	36.0	36.0	36.0	36.0
10	34.81055636011347	36.0	36.0	36.0	32.0	36.0
11	35.00981939644585	36.0	36.0	36.0	36.0	36.0
12	34.907025610646876	36.0	36.0	36.0	32.0	36.0
13	34.964369079342774	36.0	36.0	36.0	36.0	36.0
14	34.907115668405176	36.0	36.0	36.0	32.0	36.0
15	34.87271414400236	36.0	36.0	36.0	32.0	36.0
16	34.89643708319638	36.0	36.0	36.0	32.0	36.0
17	34.860200294925676	36.0	36.0	36.0	32.0	36.0
18	34.864533448233374	36.0	36.0	36.0	32.0	36.0
19	34.86174003992201	36.0	36.0	36.0	32.0	36.0
20	34.851228088529474	36.0	36.0	36.0	32.0	36.0
21	34.84873100799168	36.0	36.0	36.0	32.0	36.0
22	34.82896980126086	36.0	36.0	36.0	32.0	36.0
23	34.77507265962401	36.0	36.0	36.0	32.0	36.0
24	34.75589332308005	36.0	36.0	36.0	32.0	36.0
25	34.737912240062705	36.0	36.0	36.0	32.0	36.0
26	34.67536025125575	36.0	36.0	36.0	32.0	36.0
27	34.656720587176586	36.0	36.0	36.0	32.0	36.0
28	34.63712318310495	36.0	36.0	36.0	32.0	36.0
29	34.60638558040473	36.0	36.0	36.0	32.0	36.0
30	34.5858332674977	36.0	36.0	36.0	32.0	36.0
31	34.583739829068215	36.0	36.0	36.0	32.0	36.0
32	34.558578392447444	36.0	36.0	36.0	32.0	36.0
33	34.530871880435654	36.0	36.0	36.0	32.0	36.0
34	34.53470230113751	36.0	36.0	36.0	32.0	36.0
35	34.51625542537326	36.0	36.0	36.0	32.0	36.0
36	34.48609874914088	36.0	36.0	36.0	32.0	36.0
37	34.484241375284434	36.0	36.0	36.0	32.0	36.0
38	34.06297450529575	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	10.0
20	47.0
21	166.0
22	560.0
23	1725.0
24	4840.0
25	11783.0
26	24665.0
27	47490.0
28	82977.0
29	131683.0
30	200182.0
31	290741.0
32	425737.0
33	696324.0
34	1715201.0
35	3783329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59277536118745	18.188201735900158	12.249996157365198	26.969026745547197
2	15.858820712529434	19.896495593775875	36.88260216230295	27.362081531391734
3	17.930391824049792	23.877520370175134	28.434982208200054	29.757105597575016
4	12.134872390626363	16.195598994316235	35.611216860370035	36.05831175468737
5	14.394149586017205	36.66516210809687	33.504402721255715	15.436285584630205
6	35.193717621941566	35.48966854628938	16.248733054477242	13.067880777291807
7	30.901966906614874	30.09717376656132	20.527557103979625	18.47330222284418
8	28.322283737444074	33.41865621185025	18.599021343976897	19.660038706728784
9	27.222689979163285	14.297682614519369	17.87485700456844	40.60477040174891
10	15.189750033367533	27.10960324318932	31.93840420956698	25.762242513876178
11	37.82221350956863	20.91823788798086	22.089835128610364	19.16971347384015
12	24.85288397824318	23.249515163240172	28.6707754489585	23.226825409558145
13	29.39180699345651	19.54525330005684	25.02685559777433	26.03608410871232
14	23.52309994982111	19.300307841145663	24.93708764534284	32.23950456369039
15	25.189613374493863	27.302708123074982	21.813337230443512	25.694341271987643
16	26.03817209664764	25.760624256821067	23.26996033682689	24.931243309704403
17	24.431496919027023	25.907095985122673	24.710878195264094	24.95052890058621
18	25.08167888153657	25.20564311620336	25.42341302186651	24.28926498039356
19	25.651914361003804	25.05547045606705	24.96662605079743	24.325989132131717
20	25.880739260949365	24.533297238327613	24.552940075729406	25.033023424993615
21	26.699082987606133	24.32833786565935	24.324023718641037	24.648555428093484
22	25.879213288115665	24.382244314256035	24.738606435765366	24.999935961862928
23	24.82293110895763	24.28772036543007	25.13117712120011	25.758171404412188
24	25.043579596363287	24.943868940616078	24.791310019518807	25.221241443501835
25	25.15984595253304	24.562215013399477	24.940835494227535	25.33710353983995
26	25.01460068540983	25.163910556983115	25.128844644932364	24.69264411267469
27	25.51103458289523	24.858573421361754	24.449902142959314	25.1804898527837
28	24.926935924983507	24.756581159431622	25.116178552717898	25.200304362866977
29	24.910852256472634	24.86610649302956	25.076704134109484	25.146337116388327
30	24.831418078043406	24.946983213395633	25.31148794560727	24.91011076295369
31	25.368218940656522	24.64681315522749	24.57567022251007	25.40929768160592
32	25.169417787377945	24.749004443838068	24.432548491653883	25.649029277130104
33	24.826321995313222	24.629421761783206	24.857168125701218	25.687088117202357
34	25.325684176069927	24.628855530368742	25.084429148406826	24.961031145154504
35	25.690593359291896	24.53231307420247	25.011156107034992	24.76593745947064
36	24.8479331609653	24.981091915266973	24.8010842522685	25.369890671499228
37	25.66232223366968	24.693985624732555	24.602552732996813	25.041139408600948
38	24.94099051803773	24.58071855402637	24.965028422158984	25.51326250577692
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	50.5
2	81.0
3	81.0
4	355.0
5	629.0
6	629.0
7	871.5
8	1114.0
9	1211.5
10	1309.0
11	1309.0
12	2073.0
13	2837.0
14	4801.0
15	6765.0
16	6765.0
17	11150.5
18	15536.0
19	15536.0
20	21664.5
21	27793.0
22	35877.0
23	43961.0
24	43961.0
25	57181.5
26	70402.0
27	70402.0
28	91830.0
29	113258.0
30	138979.0
31	164700.0
32	164700.0
33	207337.5
34	249975.0
35	249975.0
36	283916.0
37	317857.0
38	359811.0
39	401765.0
40	401765.0
41	437308.5
42	472852.0
43	530711.0
44	588570.0
45	588570.0
46	603768.0
47	618966.0
48	618966.0
49	658458.0
50	697950.0
51	708581.5
52	719213.0
53	719213.0
54	699108.5
55	679004.0
56	679004.0
57	650712.0
58	622420.0
59	562179.5
60	501939.0
61	501939.0
62	455825.0
63	409711.0
64	341692.0
65	273673.0
66	273673.0
67	225291.0
68	176909.0
69	176909.0
70	139736.5
71	102564.0
72	79570.5
73	56577.0
74	56577.0
75	42521.5
76	28466.0
77	28466.0
78	25762.5
79	23059.0
80	17735.5
81	12412.0
82	12412.0
83	11055.5
84	9699.0
85	9699.0
86	6381.5
87	3064.0
88	2418.0
89	1772.0
90	1772.0
91	1069.0
92	366.0
93	246.5
94	127.0
95	127.0
96	106.0
97	85.0
98	85.0
99	73.5
100	62.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009113629432816777
2	0.0
3	0.0
4	2.831157072324739E-4
5	5.3926801377614074E-5
6	2.157072055104563E-4
7	3.774876096432985E-4
8	0.001981809950627317
9	0.003599613991955739
10	9.706824247970533E-4
11	0.005500533740516634
12	2.2918890585485978E-4
13	1.8874380482164924E-4
14	0.0
15	0.0
16	2.6963400688807037E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	6.740850172201759E-5
22	9.437190241082462E-5
23	1.3481700344403517E-4
24	0.0
25	1.0785360275522815E-4
26	2.6963400688807037E-5
27	1.2133530309963166E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.3481700344403517E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	7417462.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.79443197621868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.09514413624035	24.86534709199308
2	16.727547006450312	12.978713689414741
3	7.191274026678572	8.369441731509902
4	3.789545175165996	5.880530100751398
5	2.1709250914701435	4.210990289325241
6	1.3673148707541918	3.182652224612744
7	0.9446208205198249	2.5652219715483358
8	0.6599423631461675	2.0481671292239203
9	0.4919417052700208	1.7176139119226557
>10	2.3372010995108123	15.724718949620812
>50	0.13350240884326847	3.554467554911437
>100	0.07308872296851057	5.5005255320014275
>500	0.0092558985759684	2.5344523764128923
>1k	0.00834741037482355	6.09394169759955
>5k	3.4926403086767637E-4	0.7732157491518103
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7432	0.10019599695960694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3481700344403517E-4
2	0.0	0.0	0.0	0.0	1.3481700344403517E-4
3	0.0	1.3481700344403517E-5	0.0	0.0	1.3481700344403517E-4
4	0.0	1.3481700344403517E-5	0.0	0.0	1.3481700344403517E-4
5	0.0	5.392680137761407E-5	0.0	0.0	1.4829870378843868E-4
6	0.0	5.392680137761407E-5	0.0	0.0	1.4829870378843868E-4
7	0.0	5.392680137761407E-5	0.0	0.0	1.4829870378843868E-4
8	0.0	5.392680137761407E-5	0.0	0.0	1.4829870378843868E-4
9	0.0	5.392680137761407E-5	0.0	0.0	1.617804041328422E-4
10	0.0	5.392680137761407E-5	0.0	0.0	1.7526210447724573E-4
11	0.0	5.392680137761407E-5	0.0	0.0	1.7526210447724573E-4
12	0.0	5.392680137761407E-5	0.0	0.0	5.123046130873336E-4
13	0.0	5.392680137761407E-5	0.0	0.0	6.606033168757723E-4
14	0.0	5.392680137761407E-5	0.0	0.0	6.740850172201759E-4
15	0.0	5.392680137761407E-5	0.0	1.3481700344403517E-5	8.358654213530181E-4
16	0.0	5.392680137761407E-5	0.0	8.08902020664211E-5	8.897922227306322E-4
17	0.0	5.392680137761407E-5	0.0	1.3481700344403517E-4	8.897922227306322E-4
18	0.0	5.392680137761407E-5	0.0	1.7526210447724573E-4	8.897922227306322E-4
19	0.0	5.392680137761407E-5	0.0	2.4267060619926332E-4	9.302373237638427E-4
20	0.0	5.392680137761407E-5	0.0	3.5052420895449147E-4	0.0010515726268634743
21	0.0	5.392680137761407E-5	0.0	3.90969309987702E-4	0.0011189811285854919
22	0.0	5.392680137761407E-5	0.0	4.718595120541231E-4	0.001145944529274299
23	0.0	5.392680137761407E-5	0.0	6.875667175645794E-4	0.001145944529274299
24	0.0	5.392680137761407E-5	0.0	0.0010246092261746672	0.0011863896303075095
25	0.0	6.740850172201759E-5	0.0	0.0013481700344403517	0.0011863896303075095
26	0.0	6.740850172201759E-5	0.0	0.0016582491423616326	0.0013212066337515446
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7200	0.0	21.111778	1
GGTATCA	2415	0.0	20.20768	1
TCTAGCG	1315	0.0	15.20858	28
CTAGCGG	1340	0.0	15.044236	29
AAACGCT	1185	0.0	14.041692	29
TAAACGC	1165	0.0	14.008081	28
TATCAAC	10805	0.0	13.993491	2
TCAACGC	10635	0.0	13.901616	4
TACCGTC	1290	0.0	13.7689905	7
ACCGTCG	1275	0.0	13.679878	8
CGTCGTA	1300	0.0	13.539437	10
ATACCGT	1395	0.0	13.535572	6
CAACGCA	11005	0.0	13.449856	5
TTTTCGG	1530	0.0	13.385141	29
ATCAACG	11130	0.0	13.340583	3
AACGCAG	11160	0.0	13.291817	6
TAGCGTA	990	0.0	13.254016	7
AACCGCG	895	0.0	13.230537	7
TAGCGGC	1565	0.0	13.0857935	30
ATCGTTT	1040	0.0	12.922614	29
>>END_MODULE