FastQCFastQC Report
Thu 2 Feb 2017
SRR4062763_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062763_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881694
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30820.34955438054472415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20710.23488874825052683No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15350.17409668206883566No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12700.14404090307975329No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11850.13440037019646273No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9200.10434459120738035No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9100.10321041086816968No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8990.10196281249503795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC1600.020.9987727
ACGTAAT400.004484182719.99882918
ATACCGT1700.019.7601876
CCGTCGT1750.019.1999659
ACCGTCG1750.019.1988758
CGTCGTA1800.018.66663410
CGAGCCG1900.018.52628315
AACCGCG953.788955E-818.5252327
GTACTAA701.7734716E-518.3012541
GCTATAC701.7882641E-518.2836073
ACGCTAT1950.018.0512510
GTCCTAT2850.017.980181
TAATACC652.1033765E-417.226834
ATACGCT2050.017.1697278
ATATACG2150.017.1123666
TACGCTA2100.016.7618759
AGAACCG1354.4383341E-1016.5887995
CGCTATT2150.016.37206511
AGGCCCG2500.015.99997210
GTCGTAG2100.015.99997211
Produced by FastQC (version 0.11.5)