##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062763_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	881694
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214598261982047	32.0	32.0	32.0	32.0	32.0
2	30.8654249660313	32.0	32.0	32.0	32.0	32.0
3	30.920605107894577	32.0	32.0	32.0	32.0	32.0
4	30.980354862344534	32.0	32.0	32.0	32.0	32.0
5	30.919316679029233	32.0	32.0	32.0	32.0	32.0
6	34.55110843444551	36.0	36.0	36.0	32.0	36.0
7	34.472962274893554	36.0	36.0	36.0	32.0	36.0
8	34.46280342159525	36.0	36.0	36.0	32.0	36.0
9	34.56999026873269	36.0	36.0	36.0	32.0	36.0
10	34.338071938790556	36.0	36.0	36.0	32.0	36.0
11	34.5970098469537	36.0	36.0	36.0	32.0	36.0
12	34.42707333836909	36.0	36.0	36.0	32.0	36.0
13	34.49868435080651	36.0	36.0	36.0	32.0	36.0
14	34.404547382652034	36.0	36.0	36.0	32.0	36.0
15	34.370910996332064	36.0	36.0	36.0	32.0	36.0
16	34.37258164397172	36.0	36.0	36.0	32.0	36.0
17	34.30066893956407	36.0	36.0	36.0	32.0	36.0
18	34.328526677055756	36.0	36.0	36.0	32.0	36.0
19	34.31307573829469	36.0	36.0	36.0	32.0	36.0
20	34.284265289318064	36.0	36.0	36.0	32.0	36.0
21	34.262436854509616	36.0	36.0	36.0	32.0	36.0
22	34.239508264772134	36.0	36.0	36.0	32.0	36.0
23	34.18845086844188	36.0	36.0	36.0	32.0	36.0
24	34.174037704691195	36.0	36.0	36.0	32.0	36.0
25	34.13882140515871	36.0	36.0	36.0	32.0	36.0
26	34.08943238810744	36.0	36.0	36.0	32.0	36.0
27	34.100997625026366	36.0	36.0	36.0	32.0	36.0
28	34.08113018802442	36.0	36.0	36.0	32.0	36.0
29	34.046795146615494	36.0	36.0	36.0	32.0	36.0
30	34.02138497029582	36.0	36.0	36.0	32.0	36.0
31	34.028209333396845	36.0	36.0	36.0	32.0	36.0
32	33.980813071201574	36.0	36.0	36.0	32.0	36.0
33	33.9410589161319	36.0	36.0	36.0	32.0	36.0
34	33.94211030130635	36.0	36.0	36.0	32.0	36.0
35	33.88963064283073	36.0	36.0	36.0	32.0	36.0
36	33.84244760653923	36.0	36.0	36.0	32.0	36.0
37	33.8388772068314	36.0	36.0	36.0	32.0	36.0
38	33.232826808393845	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	3.0
10	1.0
11	8.0
12	2.0
13	3.0
14	134.0
15	278.0
16	355.0
17	437.0
18	527.0
19	679.0
20	940.0
21	1204.0
22	1835.0
23	2753.0
24	3858.0
25	5639.0
26	8127.0
27	11734.0
28	16758.0
29	22431.0
30	30832.0
31	41654.0
32	56406.0
33	84649.0
34	187827.0
35	402616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.322692913439475	17.556806375167913	11.622100067335326	25.49840064405729
2	16.226824900763255	20.2167207958004	38.101917101855825	25.454537201580525
3	18.418616785961408	25.14014769105119	28.80508637998117	27.63614914300623
4	12.14163138320617	16.111715394325987	36.928597920534806	34.818055301933036
5	14.030976803691084	37.11250640813345	34.12608938268693	14.73042740548854
6	32.89535019558962	36.18912705442824	17.73860062402673	13.176922125955407
7	29.4189367286156	31.217973582671537	21.499749346145034	17.86334034256783
8	27.898127459764773	32.86722090530685	19.99648402499745	19.23816760993093
9	27.81492856916021	14.368509638286387	18.749971645427202	39.066590147126206
10	15.534048853960977	27.05489123715959	32.25116792882751	25.159891980051924
11	36.487263562981255	21.697480840619587	22.686498435378702	19.128757161020456
12	24.48977971549704	24.45461948693287	29.198755101068645	21.856845696501445
13	29.497928121175132	20.114750211838693	25.255028829345928	25.132292837640247
14	23.701260401259493	20.106076967051713	25.906282043138575	30.286380588550223
15	24.952778335551194	27.218397220132935	23.255180769636556	24.573643674679317
16	25.15704436716054	26.18120730763908	24.343521650457465	24.31822667474291
17	23.594427283215484	26.092303084823865	25.786010125818343	24.527259506142308
18	24.37710330778645	25.07364058404327	27.03474292999352	23.514513178176752
19	25.00657873842482	25.73829324839955	26.17044830699569	23.08467970617993
20	25.35082187368144	24.35518191015875	26.134811783440938	24.159184432718874
21	26.562746345718246	24.64290858936274	24.956302877488127	23.83804218743088
22	25.263655769575404	24.919978857631556	25.516252628617963	24.300112744175074
23	23.779540956086272	24.816614962313484	25.983450826040844	25.420393255559397
24	24.136464230658852	25.77216562554235	25.769443237645177	24.321926906153617
25	24.487717005617416	24.934319784378317	25.872446525641372	24.70551668436289
26	24.065517832044478	25.51220546980197	26.453182020825132	23.96909467732842
27	24.931744582385733	25.522818917554712	25.288022549525586	24.257413950533962
28	24.004895416615152	25.036834720708317	26.35993562005397	24.598334242622563
29	23.98602548760499	25.636991623232625	26.27128929622677	24.105693592935612
30	24.24915728680377	25.427821558032655	26.40754653636937	23.915474618794207
31	24.382575319345857	25.555608486853	25.365055816053157	24.696760377747985
32	23.938564214709928	25.650722066703263	25.323602938174034	25.08711078041278
33	23.759069981636017	25.167164426275807	25.90943686430729	25.164328727780884
34	24.32671867945888	25.456851237213268	26.260637175610096	23.95579290771775
35	25.206852506845927	25.15932237228687	25.996261113997456	23.63756400686975
36	24.099985366999064	25.84380802863985	25.703603461555474	24.352603142805613
37	24.9217332123412	25.817717785843918	25.457350272232304	23.80319872958258
38	23.889105658596783	25.747931109578598	25.91285252803891	24.450110703785707
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	448.0
1	362.0
2	276.0
3	276.0
4	626.5
5	977.0
6	977.0
7	1091.5
8	1206.0
9	1140.0
10	1074.0
11	1074.0
12	1375.0
13	1676.0
14	2107.0
15	2538.0
16	2538.0
17	3849.5
18	5161.0
19	5161.0
20	6172.5
21	7184.0
22	7709.0
23	8234.0
24	8234.0
25	9406.5
26	10579.0
27	10579.0
28	13615.5
29	16652.0
30	19868.0
31	23084.0
32	23084.0
33	28389.0
34	33694.0
35	33694.0
36	36867.5
37	40041.0
38	45266.0
39	50491.0
40	50491.0
41	54438.5
42	58386.0
43	65007.5
44	71629.0
45	71629.0
46	75238.5
47	78848.0
48	78848.0
49	80511.0
50	82174.0
51	81002.5
52	79831.0
53	79831.0
54	75743.5
55	71656.0
56	71656.0
57	68869.5
58	66083.0
59	58650.0
60	51217.0
61	51217.0
62	47681.0
63	44145.0
64	35967.5
65	27790.0
66	27790.0
67	23244.5
68	18699.0
69	18699.0
70	14774.0
71	10849.0
72	8440.0
73	6031.0
74	6031.0
75	4548.0
76	3065.0
77	3065.0
78	2915.5
79	2766.0
80	2227.0
81	1688.0
82	1688.0
83	1689.0
84	1690.0
85	1690.0
86	1144.0
87	598.0
88	496.5
89	395.0
90	395.0
91	295.0
92	195.0
93	169.0
94	143.0
95	143.0
96	132.5
97	122.0
98	122.0
99	250.5
100	379.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11648032083693435
2	0.0530796398750587
3	0.014063836206212134
4	0.0028354508480266396
5	2.2683606784213117E-4
6	1.1341803392106559E-4
7	0.0
8	4.5367213568426234E-4
9	2.2683606784213117E-4
10	5.67090169605328E-4
11	0.0012475983731317214
12	0.0018146885427370494
13	0.014404090307975329
14	0.007939262374474591
15	0.024158041225186968
16	0.010888131256422295
17	0.020982336275397133
18	0.005330647594290082
19	0.007485590238790328
20	0.005670901696053279
21	0.006464827933500738
22	0.005897737763895411
23	0.008960024679764181
24	0.013043073900922544
25	0.017012705088159837
26	0.01848713952913369
27	0.008960024679764181
28	0.006011155797816476
29	0.010094205018974838
30	0.0020415246105791804
31	0.005444065628211148
32	0.006578245967421804
33	0.00873318861192205
34	0.013156491934843608
35	0.016785869020317708
36	0.014177254240133198
37	0.010661295188580165
38	0.007031918103106067
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	881694.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.474249992213785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.28987758880902	52.03472634304139
2	11.137024771239215	13.915545394557524
3	2.6818324481374525	5.026364124065097
4	1.008194792512738	2.5194485403315556
5	0.492679843944702	1.5389901868363094
6	0.2847050682332697	1.0672041364113345
7	0.1914493328373456	0.8372457436366011
8	0.12735483949914136	0.6365118464470096
9	0.09108476126452111	0.5121406931148722
>10	0.5473419162765626	6.610942740581501
>50	0.07204076719875716	3.2059388675362377
>100	0.06966610590433361	8.542203324974489
>500	0.0058359041236301975	2.5110254015308353
>1k	9.118600193172184E-4	1.0417126569352706
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3082	0.34955438054472415	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2071	0.23488874825052683	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1535	0.17409668206883566	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1270	0.14404090307975329	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1185	0.13440037019646273	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	920	0.10434459120738035	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	910	0.10321041086816968	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	899	0.10196281249503795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1341803392106559E-4
2	0.0	0.0	0.0	0.0	1.1341803392106559E-4
3	0.0	0.0	0.0	0.0	1.1341803392106559E-4
4	0.0	0.0	0.0	0.0	1.1341803392106559E-4
5	0.0	0.0	0.0	0.0	2.2683606784213117E-4
6	0.0	0.0	0.0	0.0	2.2683606784213117E-4
7	0.0	0.0	0.0	0.0	2.2683606784213117E-4
8	0.0	0.0	0.0	0.0	2.2683606784213117E-4
9	0.0	0.0	0.0	0.0	2.2683606784213117E-4
10	0.0	0.0	0.0	0.0	2.2683606784213117E-4
11	0.0	0.0	0.0	0.0	2.2683606784213117E-4
12	0.0	0.0	0.0	0.0	4.5367213568426234E-4
13	0.0	0.0	0.0	0.0	5.67090169605328E-4
14	0.0	0.0	0.0	0.0	5.67090169605328E-4
15	0.0	0.0	0.0	0.0	6.805082035263935E-4
16	0.0	0.0	0.0	2.2683606784213117E-4	6.805082035263935E-4
17	0.0	0.0	0.0	2.2683606784213117E-4	6.805082035263935E-4
18	0.0	0.0	0.0	2.2683606784213117E-4	6.805082035263935E-4
19	0.0	0.0	0.0	2.2683606784213117E-4	6.805082035263935E-4
20	0.0	0.0	0.0	2.2683606784213117E-4	6.805082035263935E-4
21	0.0	0.0	0.0	4.5367213568426234E-4	6.805082035263935E-4
22	0.0	0.0	0.0	7.939262374474591E-4	6.805082035263935E-4
23	0.0	0.0	0.0	0.002268360678421312	6.805082035263935E-4
24	0.0	0.0	0.0	0.0037427951193951645	6.805082035263935E-4
25	0.0	0.0	0.0	0.00487697545860582	6.805082035263935E-4
26	0.0	0.0	0.0	0.005670901696053279	6.805082035263935E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTC	160	0.0	20.998772	7
ACGTAAT	40	0.0044841827	19.998829	18
ATACCGT	170	0.0	19.760187	6
CCGTCGT	175	0.0	19.199965	9
ACCGTCG	175	0.0	19.198875	8
CGTCGTA	180	0.0	18.666634	10
CGAGCCG	190	0.0	18.526283	15
AACCGCG	95	3.788955E-8	18.525232	7
GTACTAA	70	1.7734716E-5	18.301254	1
GCTATAC	70	1.7882641E-5	18.283607	3
ACGCTAT	195	0.0	18.05125	10
GTCCTAT	285	0.0	17.98018	1
TAATACC	65	2.1033765E-4	17.22683	4
ATACGCT	205	0.0	17.169727	8
ATATACG	215	0.0	17.112366	6
TACGCTA	210	0.0	16.761875	9
AGAACCG	135	4.4383341E-10	16.588799	5
CGCTATT	215	0.0	16.372065	11
AGGCCCG	250	0.0	15.999972	10
GTCGTAG	210	0.0	15.999972	11
>>END_MODULE