FastQCFastQC Report
Thu 2 Feb 2017
SRR4062763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881694
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32870.3728050774985426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24600.27900836344582136No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15910.18044809196841535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14920.16921970661022984No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14500.1644561491855451No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11190.1269147799576724No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9730.1103557470051968No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA8880.10071521412190623No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8830.10014812395230091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCA350.002065820222.8577924
GACGATT350.002066501422.85649530
TAGCCGA400.004481930320.0005689
TCGGCAA450.00885013217.77727313
GTCGTAA450.00885013217.77727330
CAAGACG2150.017.1167644
ATAGGGG854.979931E-616.9416583
GTATTAG1750.016.4585441
TCTAGCG2050.016.38977828
ACCGTCG2250.016.3569478
GACGGAC2100.016.0013627
ATACCGT2300.016.0013626
GTATTAT1203.1037416E-816.001361
CCGATAA805.617115E-516.0004549
TCTACGC600.002440838416.0004543
CGCAAGA2100.016.0004542
CGATAAC805.617115E-516.00045410
CGGTCCA2500.015.36043510
CGTCGTA2400.015.33376910
CTAGCGG2200.015.27229429
Produced by FastQC (version 0.11.5)