FastQCFastQC Report
Thu 2 Feb 2017
SRR4062762_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062762_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2218382
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT50990.22985220759995348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34220.15425657077996485No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA28800.12982434945829888No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG28420.12811138929183521No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA27430.12364867727920618No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG27320.12315282038891408No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG26690.1203129127445138No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC26630.12004244534980901No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25500.11494864274953548No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC23230.10471595964987093No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT23220.10467088175075347No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG23100.1041299469613439No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA23080.10403979116310896No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT22230.10020816973812445No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22200.10007293604077205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACAC2100.015.9981973
CTAGCGG5450.015.5585829
TATACTG2300.015.3012445
TAGCGGC5650.015.00817330
TACGACG6950.014.5006555
CGTCTTA4450.014.38234715
ATCGTTT4250.014.30491529
CGACGGT7300.014.0261337
CATCGTT4350.013.9757528
CGGCATC4400.013.8178725
ACGACGG7450.013.7421796
AATAGCG5750.013.6320175
GTATTAC2004.1836756E-1113.6156551
TCTAGCG6300.013.45910428
GACGGTA7500.013.4390918
GCATCGT4800.013.3318327
GTATCAA49900.013.3219291
TACCGTC5050.013.3057317
GTATTAG7600.013.2784241
CGACCCG4950.013.2498375
Produced by FastQC (version 0.11.5)