##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062762_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2218382
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22098222939061	32.0	32.0	32.0	32.0	32.0
2	30.8195576776227	32.0	32.0	32.0	32.0	32.0
3	30.869896167567173	32.0	32.0	32.0	32.0	32.0
4	30.94764788030195	32.0	32.0	32.0	32.0	32.0
5	30.8396822549047	32.0	32.0	32.0	32.0	32.0
6	34.49210280285361	36.0	36.0	36.0	32.0	36.0
7	34.41395395382761	36.0	36.0	36.0	32.0	36.0
8	34.402969371370666	36.0	36.0	36.0	32.0	36.0
9	34.55315360474436	36.0	36.0	36.0	32.0	36.0
10	34.25570348118584	36.0	36.0	36.0	32.0	36.0
11	34.546384707412884	36.0	36.0	36.0	32.0	36.0
12	34.357494786740965	36.0	36.0	36.0	32.0	36.0
13	34.44230434614057	36.0	36.0	36.0	32.0	36.0
14	34.33782234078711	36.0	36.0	36.0	32.0	36.0
15	34.27949875179297	36.0	36.0	36.0	32.0	36.0
16	34.29929606352738	36.0	36.0	36.0	32.0	36.0
17	34.22248783122113	36.0	36.0	36.0	32.0	36.0
18	34.245485673792885	36.0	36.0	36.0	32.0	36.0
19	34.237585321193556	36.0	36.0	36.0	32.0	36.0
20	34.216478496489785	36.0	36.0	36.0	32.0	36.0
21	34.19693091631649	36.0	36.0	36.0	32.0	36.0
22	34.16963895307481	36.0	36.0	36.0	32.0	36.0
23	34.11860806659989	36.0	36.0	36.0	32.0	36.0
24	34.102242084546305	36.0	36.0	36.0	32.0	36.0
25	34.083020417583626	36.0	36.0	36.0	32.0	36.0
26	34.02388136939445	36.0	36.0	36.0	32.0	36.0
27	34.02708460490574	36.0	36.0	36.0	32.0	36.0
28	34.00873564606997	36.0	36.0	36.0	32.0	36.0
29	33.97946205838309	36.0	36.0	36.0	32.0	36.0
30	33.95190729098956	36.0	36.0	36.0	32.0	36.0
31	33.98047045098635	36.0	36.0	36.0	32.0	36.0
32	33.926961181617955	36.0	36.0	36.0	32.0	36.0
33	33.88200273893315	36.0	36.0	36.0	32.0	36.0
34	33.89336597574268	36.0	36.0	36.0	32.0	36.0
35	33.84871135809793	36.0	36.0	36.0	32.0	36.0
36	33.80766612783551	36.0	36.0	36.0	32.0	36.0
37	33.808137642660284	36.0	36.0	36.0	32.0	36.0
38	33.194102278146865	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	8.0
10	21.0
11	16.0
12	9.0
13	11.0
14	266.0
15	592.0
16	774.0
17	897.0
18	1190.0
19	1532.0
20	2058.0
21	2830.0
22	4330.0
23	6574.0
24	9735.0
25	14946.0
26	21948.0
27	31332.0
28	44583.0
29	60071.0
30	81059.0
31	109412.0
32	149086.0
33	222115.0
34	482724.0
35	970256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.92746231849783	17.508697119976024	11.676571845342009	25.887268716184135
2	16.760569478609373	19.754364315849006	37.38265531173298	26.102410893808635
3	18.336994077889102	24.475007754286498	28.564086472917705	28.623911694906695
4	12.400510296079917	15.644045944678856	36.30454578239388	35.65089797684735
5	14.650678987997972	36.56861441370373	33.50456978644278	15.276136811855526
6	33.91183460333145	35.74696083982156	17.06856727624172	13.27263728060527
7	30.165544076718977	30.502095671529972	21.38766001527239	17.94470023647866
8	28.367926973573	32.219124359046596	19.67751169211698	19.735436975263422
9	27.74141490637312	14.302914739584743	18.69589520280565	39.25977515123649
10	16.001848199695722	26.139854623316616	31.335279202118667	26.52301797486899
11	37.11667794388319	21.20728275280086	22.617843907395496	19.058195395920457
12	25.126006894372395	23.727207526739697	28.393959242331285	22.752826336556627
13	29.260173132263905	19.277994908192834	25.43739543288251	26.024436526660754
14	24.06744451191721	19.839922747442834	24.92733904702912	31.165293693610842
15	25.372005089622995	26.692430403193008	22.37824447684826	25.557320030335738
16	26.19997682750512	25.46891617192188	23.409584666841287	24.921522333731712
17	24.232175778870594	25.56342127296574	25.188160274274622	25.01624267388904
18	25.296978128362714	24.404973084772262	25.99108040036768	24.306968386497353
19	25.748866883236904	24.982666266345326	25.274161511627803	23.994305338789964
20	25.770643008324146	24.082596827672326	24.86853345775602	25.27822670624751
21	27.277903352468574	24.054726121449107	24.205160293312826	24.462210232769493
22	25.925416674743722	24.055942227473686	24.675944688910057	25.342696408872534
23	24.359856350313365	23.792407628977706	25.497143145668467	26.350592875040462
24	24.910508187261055	25.14417874918849	24.83620969487124	25.10910336867922
25	25.10297453950002	24.217141022820314	25.010595016104425	25.669289421575243
26	24.846504061814972	25.014179607154997	25.588397339193335	24.550918991836696
27	25.609788534510393	24.738806962623077	24.36629553775223	25.2851089651143
28	24.65499924264828	24.405884622874908	25.527080733693495	25.412035400783317
29	24.800803188952276	24.686203597759942	25.53654879408951	24.97644441919827
30	24.808740311920875	24.67399864491987	25.74115805706934	24.776102986089917
31	25.218853473322618	24.61919563262279	24.53354310688759	25.628407787166996
32	24.757205178360593	24.798499344748134	24.40661049277555	26.03768498411572
33	24.5248620727275	24.290347390088712	25.100329186123147	26.084461351060632
34	25.385666175257317	24.398248959699924	25.330979978450124	24.88510488659264
35	26.167101811196115	24.26920741177897	25.103684023305288	24.460006753719618
36	24.855684957692834	25.03692409236163	24.624537434447337	25.4828535154982
37	26.002448867714257	24.813900480035777	24.553640662023795	24.630009990226167
38	24.852417778839673	24.644191042057834	25.116634471690585	25.386756707411905
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	381.0
1	381.5
2	382.0
3	382.0
4	869.5
5	1357.0
6	1357.0
7	1498.0
8	1639.0
9	1619.0
10	1599.0
11	1599.0
12	2172.0
13	2745.0
14	3757.5
15	4770.0
16	4770.0
17	6991.5
18	9213.0
19	9213.0
20	11217.5
21	13222.0
22	14845.5
23	16469.0
24	16469.0
25	19246.5
26	22024.0
27	22024.0
28	28272.5
29	34521.0
30	41776.0
31	49031.0
32	49031.0
33	62632.5
34	76234.0
35	76234.0
36	84234.0
37	92234.0
38	104993.5
39	117753.0
40	117753.0
41	125985.5
42	134218.0
43	153146.5
44	172075.0
45	172075.0
46	176866.5
47	181658.0
48	181658.0
49	194430.0
50	207202.0
51	207468.5
52	207735.0
53	207735.0
54	198724.0
55	189713.0
56	189713.0
57	184786.0
58	179859.0
59	162548.5
60	145238.0
61	145238.0
62	137891.0
63	130544.0
64	107728.0
65	84912.0
66	84912.0
67	70084.0
68	55256.0
69	55256.0
70	44233.0
71	33210.0
72	26098.0
73	18986.0
74	18986.0
75	14295.5
76	9605.0
77	9605.0
78	9309.5
79	9014.0
80	6962.5
81	4911.0
82	4911.0
83	5038.5
84	5166.0
85	5166.0
86	3473.5
87	1781.0
88	1498.0
89	1215.0
90	1215.0
91	919.5
92	624.0
93	534.0
94	444.0
95	444.0
96	412.0
97	380.0
98	380.0
99	721.0
100	1062.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12198079501185999
2	0.05391316734448801
3	0.011449786375836083
4	0.0027948297452828233
5	3.1554529382225426E-4
6	2.7046739470478934E-4
7	0.0
8	3.1554529382225426E-4
9	9.015579823492978E-5
10	3.1554529382225426E-4
11	0.00202850546028592
12	0.0022989728549907096
13	0.013929070827296651
14	0.009150813520845373
15	0.024161753926961183
16	0.01059330629260425
17	0.020690755694916386
18	0.005454425793213252
19	0.007392775455264243
20	0.00617567217909269
21	0.006130594279975225
22	0.0074378533543817075
23	0.009376203016432697
24	0.013613525533474398
25	0.016137887884052432
26	0.018391782839925677
27	0.008790190327905654
28	0.005679815288800577
29	0.010097449402312136
30	0.0028399076444002884
31	0.005274114196743392
32	0.006806762766737199
33	0.00829433343761354
34	0.01226118855995045
35	0.015551875195525387
36	0.014334771919353836
37	0.009646670411137486
38	0.005724893187918041
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2218382.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.84585405171086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.13951835497019	33.989895379661014
2	14.384603750471214	13.189488882715919
3	4.88891373460578	6.724092766444239
4	2.2645006048632723	4.152718569222902
5	1.1915382078090295	2.7313543386124945
6	0.692588775402271	1.9051394348967403
7	0.4693095325671943	1.5061127434606563
8	0.3221546881987162	1.1815565453786208
9	0.24434291721411802	1.0081898749050953
>10	1.1884900389182103	9.6909317330905
>50	0.09328307044622451	3.004837666555571
>100	0.09595631535840268	9.51039249033401
>500	0.016302794876315058	5.010175600563063
>1k	0.008398409481738056	6.164139918851964
>5k	9.880481743221243E-5	0.23097405530736453
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5099	0.22985220759995348	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3422	0.15425657077996485	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2880	0.12982434945829888	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2842	0.12811138929183521	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2743	0.12364867727920618	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2732	0.12315282038891408	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2669	0.1203129127445138	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2663	0.12004244534980901	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2550	0.11494864274953548	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2323	0.10471595964987093	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2322	0.10467088175075347	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2310	0.1041299469613439	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2308	0.10403979116310896	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2223	0.10020816973812445	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2220	0.10007293604077205	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.507789911746489E-5
6	0.0	0.0	0.0	0.0	1.8031159646985956E-4
7	0.0	0.0	0.0	9.015579823492978E-5	2.7046739470478934E-4
8	0.0	0.0	0.0	1.3523369735239467E-4	2.7046739470478934E-4
9	0.0	0.0	0.0	2.2538949558732445E-4	2.7046739470478934E-4
10	0.0	0.0	0.0	2.2538949558732445E-4	2.7046739470478934E-4
11	0.0	0.0	0.0	2.2538949558732445E-4	2.7046739470478934E-4
12	0.0	0.0	0.0	2.7046739470478934E-4	4.958568902921138E-4
13	0.0	0.0	0.0	2.7046739470478934E-4	5.860126885270436E-4
14	0.0	0.0	0.0	2.7046739470478934E-4	5.860126885270436E-4
15	0.0	0.0	0.0	2.7046739470478934E-4	6.310905876445085E-4
16	0.0	0.0	0.0	2.7046739470478934E-4	6.310905876445085E-4
17	0.0	0.0	0.0	2.7046739470478934E-4	6.310905876445085E-4
18	0.0	0.0	0.0	4.507789911746489E-4	6.310905876445085E-4
19	0.0	0.0	0.0	5.860126885270436E-4	6.310905876445085E-4
20	0.0	0.0	0.0	6.761684867619734E-4	6.761684867619734E-4
21	0.0	0.0	0.0	7.212463858794383E-4	8.114021841143681E-4
22	0.0	0.0	0.0	0.001262181175289017	8.56480083231833E-4
23	0.0	0.0	0.0	0.0019383496620509902	8.56480083231833E-4
24	0.0	0.0	0.0	0.0030652971399876125	8.56480083231833E-4
25	0.0	0.0	0.0	0.0037865435258670507	8.56480083231833E-4
26	0.0	0.0	0.0	0.005048724701156068	8.56480083231833E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACAC	210	0.0	15.998197	3
CTAGCGG	545	0.0	15.55858	29
TATACTG	230	0.0	15.301244	5
TAGCGGC	565	0.0	15.008173	30
TACGACG	695	0.0	14.500655	5
CGTCTTA	445	0.0	14.382347	15
ATCGTTT	425	0.0	14.304915	29
CGACGGT	730	0.0	14.026133	7
CATCGTT	435	0.0	13.97575	28
CGGCATC	440	0.0	13.81787	25
ACGACGG	745	0.0	13.742179	6
AATAGCG	575	0.0	13.632017	5
GTATTAC	200	4.1836756E-11	13.615655	1
TCTAGCG	630	0.0	13.459104	28
GACGGTA	750	0.0	13.439091	8
GCATCGT	480	0.0	13.33183	27
GTATCAA	4990	0.0	13.321929	1
TACCGTC	505	0.0	13.305731	7
GTATTAG	760	0.0	13.278424	1
CGACCCG	495	0.0	13.249837	5
>>END_MODULE