FastQCFastQC Report
Thu 2 Feb 2017
SRR4062762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2218382
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT53260.24008489069961803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT38080.1716566398393063No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG30170.13600002163739158No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA30040.13541400894886454No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA29060.13099637483535298No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28280.12748029870419073No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA26670.12022275694627887No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26350.11878026417451999No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG25070.11301029308748449No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24150.10886312636867772No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC23870.1076009451933887No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG23580.10629368611898221No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT23060.10394963536487403No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT23010.10372424586928672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT5400.019.5558596
ACCGTCG5400.018.9632578
TACCGTC5500.018.9093847
CGTCGTA5950.017.74777610
CCGTCGT6150.017.170619
GTCGTAG6400.016.24988611
AGATACC6750.015.4076464
CTAGCGG6400.015.24989329
TCTAGCG6500.015.01527928
TCAGATA7300.014.904342
GTATTAC2150.014.8846231
TAAGGCG650.004162771614.7694614
CAGATAC7400.014.702933
AACCGCG4650.014.4518377
GCATTCG7600.014.31568922
GTTATAT3050.014.1647921
TAGCGGC7050.014.07082330
TTACACA4950.013.8992054
ATCACGA6450.013.89137525
GCTCGTA8100.013.82706326
Produced by FastQC (version 0.11.5)