##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062762_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2218382
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.140686770808635	32.0	32.0	32.0	32.0	32.0
2	31.19671003461081	32.0	32.0	32.0	32.0	32.0
3	31.312276695357248	32.0	32.0	32.0	32.0	32.0
4	31.431772345790762	32.0	32.0	32.0	32.0	32.0
5	31.333521458432315	32.0	32.0	32.0	32.0	32.0
6	34.84611577266674	36.0	36.0	36.0	32.0	36.0
7	34.87268558796456	36.0	36.0	36.0	32.0	36.0
8	34.81583108770266	36.0	36.0	36.0	32.0	36.0
9	34.94636225861912	36.0	36.0	36.0	32.0	36.0
10	34.7616100383072	36.0	36.0	36.0	32.0	36.0
11	34.96162202902836	36.0	36.0	36.0	36.0	36.0
12	34.84851932624769	36.0	36.0	36.0	32.0	36.0
13	34.91764357987037	36.0	36.0	36.0	32.0	36.0
14	34.854837895366984	36.0	36.0	36.0	32.0	36.0
15	34.820545334392364	36.0	36.0	36.0	32.0	36.0
16	34.83538317566587	36.0	36.0	36.0	32.0	36.0
17	34.80122314371465	36.0	36.0	36.0	32.0	36.0
18	34.80259035639489	36.0	36.0	36.0	32.0	36.0
19	34.79774853925068	36.0	36.0	36.0	32.0	36.0
20	34.78013840718145	36.0	36.0	36.0	32.0	36.0
21	34.772476516668455	36.0	36.0	36.0	32.0	36.0
22	34.75730960673139	36.0	36.0	36.0	32.0	36.0
23	34.69240329212913	36.0	36.0	36.0	32.0	36.0
24	34.66313511379014	36.0	36.0	36.0	32.0	36.0
25	34.64851815422231	36.0	36.0	36.0	32.0	36.0
26	34.583372025196745	36.0	36.0	36.0	32.0	36.0
27	34.55809188859268	36.0	36.0	36.0	32.0	36.0
28	34.532204101908505	36.0	36.0	36.0	32.0	36.0
29	34.497223201414364	36.0	36.0	36.0	32.0	36.0
30	34.47736773919009	36.0	36.0	36.0	32.0	36.0
31	34.47206161968498	36.0	36.0	36.0	32.0	36.0
32	34.44479264617185	36.0	36.0	36.0	32.0	36.0
33	34.41015343615302	36.0	36.0	36.0	32.0	36.0
34	34.39633706007351	36.0	36.0	36.0	32.0	36.0
35	34.36084632854035	36.0	36.0	36.0	32.0	36.0
36	34.33553012961699	36.0	36.0	36.0	32.0	36.0
37	34.3284758891841	36.0	36.0	36.0	32.0	36.0
38	33.87128366530201	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	19.0
21	47.0
22	182.0
23	550.0
24	1511.0
25	3622.0
26	7985.0
27	15423.0
28	26928.0
29	42724.0
30	65197.0
31	95010.0
32	137594.0
33	220489.0
34	524410.0
35	1076690.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.268160680917056	18.75010425189964	12.407351055029224	26.574384012154077
2	15.623008120332745	20.920698058314574	36.59671778800946	26.85957603334322
3	17.245001086377368	25.263547937190257	28.972467320777035	28.51898365565534
4	11.91676444827507	16.59896708362277	36.337388696881824	35.146879771220334
5	14.240076091562312	37.02431504070538	33.18782174379502	15.547787123937287
6	33.89347181276427	36.24216770796708	16.72774727503854	13.136613204230112
7	29.97486445014632	30.714686265423474	21.28763796153215	18.022811322898054
8	28.22632449442984	32.52522046920734	19.460749200865333	19.787705835497494
9	27.626716290207554	14.13720945119285	18.48463374238494	39.75144051621465
10	15.770604794994489	26.404249996055633	31.36238338769043	26.462761821259445
11	37.27788484295229	21.145078946478684	22.43477476868287	19.14226144188615
12	24.9174735245871	23.84948649441777	28.321129978240865	22.91191000275426
13	29.209120713818514	19.257349623306027	25.422797457062117	26.110732205813342
14	23.85644131623859	19.768056177880997	24.937905193965694	31.437597311914722
15	25.290459442963385	26.798901181131114	22.274522602509396	25.636116773396108
16	26.15619228617627	25.506394077482632	23.309386439930744	25.028027196410356
17	24.204668086921007	25.75241775311917	25.03410143068236	25.008812729277462
18	25.313945028403584	24.376505038356786	25.993539435498487	24.316010497741146
19	25.73898453918216	24.99501889214752	25.20449588934638	24.061500679323938
20	25.76562557756058	23.992441337875984	24.845720890270474	25.396212194292957
21	27.19273848156695	24.09146498411678	24.161696445918395	24.554100088397877
22	25.854924895712276	24.14164918395479	24.698406315954603	25.305019604378327
23	24.329250014086888	23.741883135177776	25.46841719727278	26.460449653462558
24	24.960985078313836	25.09513690608741	24.793791150487156	25.150086865111597
25	25.13260796950569	24.161990208174338	25.02793704057148	25.677464781748494
26	24.963216401157602	25.015551889216454	25.43328915695237	24.58794255267357
27	25.72670146683616	24.592315112830082	24.34222270305358	25.338760717280177
28	24.647287978355394	24.338143746207823	25.62881415373908	25.38575412169771
29	24.872091461254193	24.498666144965114	25.544698793985887	25.084543599794806
30	24.812002621730613	24.52399992426913	25.83928286471852	24.824714589281736
31	25.262691457107028	24.40999791740106	24.6465216540704	25.680788971421514
32	24.847208460941353	24.64593564138187	24.4864951122034	26.02036078547338
33	24.466435447096128	24.227702893370033	25.209454458249304	26.09640720128454
34	25.307318577233318	24.261826863001954	25.478434282283214	24.952420277481515
35	26.253323368112436	24.127224256237202	25.10374678481885	24.515705590831516
36	24.969820346540857	24.850544225476046	24.678887585636737	25.50074784234636
37	26.068909682822888	24.683440453447602	24.568536888597183	24.679112975132327
38	24.947878655650225	24.468970839544696	25.18192321337047	25.40122729143461
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	249.0
1	221.0
2	193.0
3	193.0
4	716.5
5	1240.0
6	1240.0
7	1413.0
8	1586.0
9	1620.0
10	1654.0
11	1654.0
12	2179.5
13	2705.0
14	3735.5
15	4766.0
16	4766.0
17	7109.5
18	9453.0
19	9453.0
20	11298.0
21	13143.0
22	14958.0
23	16773.0
24	16773.0
25	19395.5
26	22018.0
27	22018.0
28	28221.5
29	34425.0
30	41805.0
31	49185.0
32	49185.0
33	63271.0
34	77357.0
35	77357.0
36	85084.0
37	92811.0
38	105211.0
39	117611.0
40	117611.0
41	125679.0
42	133747.0
43	152979.5
44	172212.0
45	172212.0
46	176972.0
47	181732.0
48	181732.0
49	194921.5
50	208111.0
51	208018.5
52	207926.0
53	207926.0
54	198909.5
55	189893.0
56	189893.0
57	185197.5
58	180502.0
59	162778.0
60	145054.0
61	145054.0
62	138305.5
63	131557.0
64	108221.5
65	84886.0
66	84886.0
67	70206.0
68	55526.0
69	55526.0
70	44580.0
71	33634.0
72	26276.5
73	18919.0
74	18919.0
75	14028.5
76	9138.0
77	9138.0
78	9096.0
79	9054.0
80	6959.0
81	4864.0
82	4864.0
83	4587.0
84	4310.0
85	4310.0
86	2704.5
87	1099.0
88	934.5
89	770.0
90	770.0
91	454.0
92	138.0
93	107.0
94	76.0
95	76.0
96	56.5
97	37.0
98	37.0
99	32.5
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008880346126140583
2	0.0
3	0.0
4	1.3523369735239467E-4
5	9.015579823492978E-5
6	9.015579823492978E-5
7	4.507789911746489E-4
8	0.00202850546028592
9	0.0036062319293971913
10	0.001217103276171552
11	0.004823335205568743
12	1.3523369735239467E-4
13	1.3523369735239467E-4
14	0.0
15	0.0
16	4.507789911746489E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.3523369735239467E-4
22	0.0
23	3.1554529382225426E-4
24	0.0
25	4.507789911746489E-5
26	9.015579823492978E-5
27	9.015579823492978E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.507789911746489E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2218382.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.545260914109704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.90403842637203	31.3108011405454
2	15.443623039561505	13.449931894337237
3	5.469473016471782	7.145088886948393
4	2.41264594852232	4.202371892870965
5	1.3070718524829261	2.845839242492886
6	0.7929053017810035	2.0716360947740817
7	0.5239125109651896	1.5969734890301883
8	0.3464424332664677	1.2068740918645902
9	0.2708962331831374	1.06166224391483
>10	1.29821044048666	10.064571549252104
>50	0.1010535131191543	3.0997251390969587
>100	0.10218402131309152	9.712628238907204
>500	0.017773199559250698	5.193580682777387
>1k	0.009666126076083711	6.797262954242135
>5k	1.0393683952778184E-4	0.24105245894565072
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5326	0.24008489069961803	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3808	0.1716566398393063	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3017	0.13600002163739158	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3004	0.13541400894886454	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2906	0.13099637483535298	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2828	0.12748029870419073	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2667	0.12022275694627887	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2635	0.11878026417451999	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2507	0.11301029308748449	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2415	0.10886312636867772	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2387	0.1076009451933887	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2358	0.10629368611898221	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2306	0.10394963536487403	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	2301	0.10372424586928672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.015579823492978E-5
7	0.0	0.0	0.0	0.0	9.015579823492978E-5
8	0.0	0.0	0.0	0.0	9.015579823492978E-5
9	0.0	0.0	0.0	0.0	9.015579823492978E-5
10	0.0	0.0	0.0	0.0	1.3523369735239467E-4
11	0.0	0.0	0.0	0.0	1.3523369735239467E-4
12	0.0	0.0	0.0	0.0	2.7046739470478934E-4
13	0.0	0.0	0.0	0.0	3.606231929397191E-4
14	0.0	0.0	0.0	0.0	3.606231929397191E-4
15	0.0	0.0	0.0	0.0	5.860126885270436E-4
16	0.0	0.0	0.0	4.507789911746489E-5	6.761684867619734E-4
17	0.0	0.0	0.0	9.015579823492978E-5	6.761684867619734E-4
18	0.0	0.0	0.0	2.7046739470478934E-4	6.761684867619734E-4
19	0.0	0.0	0.0	3.606231929397191E-4	6.761684867619734E-4
20	0.0	0.0	0.0	4.507789911746489E-4	6.761684867619734E-4
21	0.0	0.0	0.0	5.409347894095787E-4	6.761684867619734E-4
22	0.0	0.0	0.0	0.0010818695788191574	8.114021841143681E-4
23	0.0	0.0	0.0	0.0018031159646985956	8.114021841143681E-4
24	0.0	0.0	0.0	0.002884985543517753	8.114021841143681E-4
25	0.0	0.0	0.0	0.003335764534692402	8.114021841143681E-4
26	0.0	0.0	0.0	0.004643023609098884	8.56480083231833E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	540	0.0	19.555859	6
ACCGTCG	540	0.0	18.963257	8
TACCGTC	550	0.0	18.909384	7
CGTCGTA	595	0.0	17.747776	10
CCGTCGT	615	0.0	17.17061	9
GTCGTAG	640	0.0	16.249886	11
AGATACC	675	0.0	15.407646	4
CTAGCGG	640	0.0	15.249893	29
TCTAGCG	650	0.0	15.015279	28
TCAGATA	730	0.0	14.90434	2
GTATTAC	215	0.0	14.884623	1
TAAGGCG	65	0.0041627716	14.769461	4
CAGATAC	740	0.0	14.70293	3
AACCGCG	465	0.0	14.451837	7
GCATTCG	760	0.0	14.315689	22
GTTATAT	305	0.0	14.164792	1
TAGCGGC	705	0.0	14.070823	30
TTACACA	495	0.0	13.899205	4
ATCACGA	645	0.0	13.891375	25
GCTCGTA	810	0.0	13.827063	26
>>END_MODULE