FastQCFastQC Report
Thu 2 Feb 2017
SRR4062761_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062761_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927919
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT67530.23064162635646682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT44390.15160938536892585No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA33610.11479142694862801No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA33230.11349357683733738No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG33160.11325449918525753No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG33060.1129129596822863No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG32910.11240065042782946No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC32310.11035141341000211No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA32020.10936094885138559No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC29440.10054922967472801No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA550.001367264517.4508574
GTCCTAC14800.016.6684721
CGTCGTA6700.015.99989610
GTCCTAT9950.015.9385531
ACCGTCG6750.015.6430098
TACCGTC7100.015.5476047
GTCCTAA11650.015.400291
CATCGTT6150.015.34870928
ATACCGT7650.015.2644866
CCGTCGT6850.015.1823849
TCCTACA17000.015.1593172
ATCGTTT6500.014.76938829
TAGGACG20400.014.6635684
GTAGGAC19050.014.4446223
CTATTCC9650.014.4218224
CGTTTAT6700.014.0894631
CGCGGTT8200.014.0486910
CCTATTC10300.013.979063
TCTAGCG7100.013.971024528
TCCATTA9850.013.9682618
Produced by FastQC (version 0.11.5)