##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062761_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2927919
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.245120852045428	32.0	32.0	32.0	32.0	32.0
2	30.913196710701357	32.0	32.0	32.0	32.0	32.0
3	30.963100755177994	32.0	32.0	32.0	32.0	32.0
4	31.017450619364812	32.0	32.0	32.0	32.0	32.0
5	30.9591699770383	32.0	32.0	32.0	32.0	32.0
6	34.59036913247942	36.0	36.0	36.0	32.0	36.0
7	34.52315928138722	36.0	36.0	36.0	32.0	36.0
8	34.5123167683259	36.0	36.0	36.0	32.0	36.0
9	34.625897779275995	36.0	36.0	36.0	32.0	36.0
10	34.385671188308145	36.0	36.0	36.0	32.0	36.0
11	34.61517548811972	36.0	36.0	36.0	32.0	36.0
12	34.457654737033366	36.0	36.0	36.0	32.0	36.0
13	34.52199463168209	36.0	36.0	36.0	32.0	36.0
14	34.44043226605654	36.0	36.0	36.0	32.0	36.0
15	34.39732998078157	36.0	36.0	36.0	32.0	36.0
16	34.405251306473986	36.0	36.0	36.0	32.0	36.0
17	34.34309794772328	36.0	36.0	36.0	32.0	36.0
18	34.36293695283237	36.0	36.0	36.0	32.0	36.0
19	34.35065553384503	36.0	36.0	36.0	32.0	36.0
20	34.31640697710558	36.0	36.0	36.0	32.0	36.0
21	34.296614079829396	36.0	36.0	36.0	32.0	36.0
22	34.27146276929109	36.0	36.0	36.0	32.0	36.0
23	34.22582626090408	36.0	36.0	36.0	32.0	36.0
24	34.205435327958185	36.0	36.0	36.0	32.0	36.0
25	34.173579938516056	36.0	36.0	36.0	32.0	36.0
26	34.13836311728569	36.0	36.0	36.0	32.0	36.0
27	34.13269424461537	36.0	36.0	36.0	32.0	36.0
28	34.1045233833313	36.0	36.0	36.0	32.0	36.0
29	34.07253752579904	36.0	36.0	36.0	32.0	36.0
30	34.04153222817981	36.0	36.0	36.0	32.0	36.0
31	34.05596261371985	36.0	36.0	36.0	32.0	36.0
32	34.00952621981688	36.0	36.0	36.0	32.0	36.0
33	33.97499350221096	36.0	36.0	36.0	32.0	36.0
34	33.97001351471813	36.0	36.0	36.0	32.0	36.0
35	33.93475365951039	36.0	36.0	36.0	32.0	36.0
36	33.893515155303135	36.0	36.0	36.0	32.0	36.0
37	33.87530802593924	36.0	36.0	36.0	32.0	36.0
38	33.264362163024316	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	6.0
8	4.0
9	7.0
10	20.0
11	30.0
12	14.0
13	14.0
14	327.0
15	726.0
16	1063.0
17	1312.0
18	1693.0
19	2227.0
20	2883.0
21	3772.0
22	5529.0
23	8009.0
24	12131.0
25	18081.0
26	25876.0
27	37865.0
28	53554.0
29	73459.0
30	99649.0
31	135931.0
32	185827.0
33	281625.0
34	636909.0
35	1339375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.90075627941714	17.472256760213327	11.811685194076153	25.81530176629338
2	16.100058948663403	19.721386404216965	38.66321517970799	25.51533946741164
3	18.49516063746027	24.454365502953827	28.673005289396787	28.37746857018912
4	12.17127598676975	15.844910712212023	36.62735890944712	35.35645439157111
5	14.285245887081874	36.90644073979075	33.86286937196477	14.945444001162606
6	33.60313889322455	36.02189410812009	17.37877278373557	12.996194214919784
7	29.947252621145807	30.668857529479993	21.328910167566168	18.054979681808028
8	27.77758613564415	33.54398408968032	19.568764214486027	19.1096655601895
9	27.72590159813546	14.237893761834371	18.725016325627273	39.31118831440289
10	15.726285022031094	26.765911622192917	31.973829769866324	25.533973585909663
11	37.08217919511454	20.96592403351242	23.030257490940514	18.921639280432533
12	25.251925646413337	23.488992597976395	29.259749823078245	21.99933193253202
13	29.707919638788553	19.8225642377989	25.23691008176993	25.23260604164261
14	23.363623756599985	20.41461126948571	25.420865415290994	30.800899558623307
15	24.766965900465078	28.19054991469821	22.528690711499582	24.513793473337127
16	25.199208358553182	25.708842910956047	24.79433581501222	24.297612915478545
17	23.593416826595597	26.271545996333877	25.817823814231478	24.317213362839055
18	24.5174126294505	24.63285925075826	27.468781594119733	23.380946525671504
19	25.563923899306623	25.077876831642588	25.55808313693343	23.80011613211736
20	25.73760642569184	24.537673507990956	25.53171431821124	24.193005748105968
21	26.768076810755144	24.357288875454067	24.43537024607928	24.43926406771151
22	25.584278781519494	25.059432075286093	25.014482298805348	24.341806844389065
23	23.693318855168364	24.797216312050683	25.94903654051558	25.560428292265374
24	24.797268400924196	25.33642856264492	25.58424958223969	24.2820534541912
25	24.684057563079175	24.902064334884635	25.710725304635602	24.703152797400588
26	23.89482346519058	25.958646340434445	26.274901507592276	23.8716286867827
27	24.91790343791095	25.41167830854098	25.144262463067648	24.52615579048042
28	23.8005098806453	25.30412495081537	25.788593538232853	25.106771630306472
29	24.180300290986523	25.255983771156636	25.703308515736467	24.860407422120375
30	23.92589612259703	25.427619579785205	26.261682689877436	24.384801607740325
31	24.985372661547125	25.054265461685937	24.968021376237004	24.99234050052993
32	24.20635205271005	25.58305709401359	24.966262084936545	25.244328768339813
33	23.645526989039066	25.163081904722866	25.998353639583698	25.19303746665437
34	24.645152475213706	25.274694494496202	26.12046011562668	23.95969291466341
35	25.17290004078685	25.182567276101025	25.836762412149426	23.8077702709627
36	23.86284411175265	25.826566754375147	25.655670143238208	24.65491899063399
37	24.991793655787156	25.706368977047173	24.95664556756648	24.345191799599196
38	24.17360777613752	25.816862643567056	25.470313820350675	24.53921575994475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	211.0
1	293.5
2	376.0
3	376.0
4	907.5
5	1439.0
6	1439.0
7	1724.0
8	2009.0
9	2063.5
10	2118.0
11	2118.0
12	2735.0
13	3352.0
14	4552.5
15	5753.0
16	5753.0
17	8746.0
18	11739.0
19	11739.0
20	14458.5
21	17178.0
22	19462.0
23	21746.0
24	21746.0
25	27452.0
26	33158.0
27	33158.0
28	43172.0
29	53186.0
30	67877.0
31	82568.0
32	82568.0
33	104167.5
34	125767.0
35	125767.0
36	138526.5
37	151286.0
38	167344.0
39	183402.0
40	183402.0
41	188262.5
42	193123.0
43	211084.5
44	229046.0
45	229046.0
46	234915.5
47	240785.0
48	240785.0
49	252227.0
50	263669.0
51	260907.0
52	258145.0
53	258145.0
54	246225.0
55	234305.0
56	234305.0
57	226870.5
58	219436.0
59	196084.5
60	172733.0
61	172733.0
62	163023.5
63	153314.0
64	126409.0
65	99504.0
66	99504.0
67	82333.5
68	65163.0
69	65163.0
70	52755.5
71	40348.0
72	31560.0
73	22772.0
74	22772.0
75	16936.5
76	11101.0
77	11101.0
78	10882.5
79	10664.0
80	8427.5
81	6191.0
82	6191.0
83	6167.0
84	6143.0
85	6143.0
86	4105.5
87	2068.0
88	1758.5
89	1449.0
90	1449.0
91	1050.5
92	652.0
93	560.5
94	469.0
95	469.0
96	429.5
97	390.0
98	390.0
99	775.5
100	1161.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12305668292053162
2	0.056149094288469044
3	0.012432037908152513
4	0.00283477787466115
5	3.415395029712229E-4
6	2.7323160237697835E-4
7	3.4153950297122294E-5
8	2.7323160237697835E-4
9	2.3907765207985601E-4
10	4.0984740356546747E-4
11	0.0016735435645589924
12	0.0024249304710956825
13	0.014925276279842442
14	0.008914181027548918
15	0.022712376947586325
16	0.011304957548347478
17	0.01970682932143956
18	0.005430478097242444
19	0.0074797151150697815
20	0.005874479451105034
21	0.006967405860612947
22	0.007069867711504314
23	0.009972953486759709
24	0.013764041969740282
25	0.016632973794698556
26	0.01830651735925755
27	0.009187412629925895
28	0.005976941301996401
29	0.009972953486759709
30	0.00283477787466115
31	0.006216018954076257
32	0.006967405860612947
33	0.00850433362398345
34	0.013456656417066181
35	0.017247744900046758
36	0.014276351224197119
37	0.01045110879091942
38	0.006591712407344601
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2927919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.02612564282143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.61769934122253	34.14929053112961
2	14.989068088621918	14.09755598408679
3	5.579954394913357	7.872109093692274
4	2.4896808851679073	4.683201844657475
5	1.3364126577568545	3.142315477716537
6	0.7840852249116468	2.2123494180825
7	0.4770834646748403	1.570477086633812
8	0.3175962606654805	1.1948257326196121
9	0.22781323478438648	0.9641856421861321
>10	1.0045222979350958	8.286594429577065
>50	0.07574457230858815	2.5050082654228825
>100	0.07840149634069993	7.946237451881349
>500	0.013920841903916629	4.5642345058240155
>1k	0.007944354803768575	6.580158728236217
>5k	7.288398902539977E-5	0.23145580825370432
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6753	0.23064162635646682	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4439	0.15160938536892585	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3361	0.11479142694862801	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3323	0.11349357683733738	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3316	0.11325449918525753	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3306	0.1129129596822863	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3291	0.11240065042782946	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3231	0.11035141341000211	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3202	0.10936094885138559	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2944	0.10054922967472801	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.4153950297122294E-5	0.0
5	0.0	0.0	0.0	3.4153950297122294E-5	0.0
6	0.0	0.0	0.0	3.4153950297122294E-5	0.0
7	0.0	0.0	0.0	6.830790059424459E-5	3.4153950297122294E-5
8	0.0	0.0	0.0	1.0246185089136687E-4	3.4153950297122294E-5
9	0.0	0.0	0.0	1.3661580118848917E-4	3.4153950297122294E-5
10	0.0	0.0	0.0	1.3661580118848917E-4	3.4153950297122294E-5
11	0.0	0.0	0.0	1.3661580118848917E-4	6.830790059424459E-5
12	0.0	0.0	0.0	1.7076975148561145E-4	3.756934532683452E-4
13	0.0	0.0	0.0	1.7076975148561145E-4	4.440013538625898E-4
14	0.0	0.0	0.0	1.7076975148561145E-4	4.440013538625898E-4
15	0.0	0.0	0.0	2.3907765207985604E-4	5.806171550510789E-4
16	0.0	0.0	0.0	2.3907765207985604E-4	5.806171550510789E-4
17	0.0	0.0	0.0	2.3907765207985604E-4	5.806171550510789E-4
18	0.0	0.0	0.0	2.7323160237697835E-4	5.806171550510789E-4
19	0.0	0.0	0.0	3.415395029712229E-4	6.147711053482013E-4
20	0.0	0.0	0.0	5.464632047539567E-4	7.172329562395682E-4
21	0.0	0.0	0.0	6.147711053482013E-4	7.513869065366904E-4
22	0.0	0.0	0.0	8.880027077251796E-4	7.855408568338127E-4
23	0.0	0.0	0.0	0.001297850111290647	7.855408568338127E-4
24	0.0	0.0	0.0	0.0019467751669359707	7.855408568338127E-4
25	0.0	0.0	0.0	0.0023907765207985604	7.855408568338127E-4
26	0.0	0.0	0.0	0.003142163427335251	8.196948071309349E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	55	0.0013672645	17.450857	4
GTCCTAC	1480	0.0	16.668472	1
CGTCGTA	670	0.0	15.999896	10
GTCCTAT	995	0.0	15.938553	1
ACCGTCG	675	0.0	15.643009	8
TACCGTC	710	0.0	15.547604	7
GTCCTAA	1165	0.0	15.40029	1
CATCGTT	615	0.0	15.348709	28
ATACCGT	765	0.0	15.264486	6
CCGTCGT	685	0.0	15.182384	9
TCCTACA	1700	0.0	15.159317	2
ATCGTTT	650	0.0	14.769388	29
TAGGACG	2040	0.0	14.663568	4
GTAGGAC	1905	0.0	14.444622	3
CTATTCC	965	0.0	14.421822	4
CGTTTAT	670	0.0	14.08946	31
CGCGGTT	820	0.0	14.04869	10
CCTATTC	1030	0.0	13.97906	3
TCTAGCG	710	0.0	13.9710245	28
TCCATTA	985	0.0	13.968261	8
>>END_MODULE