FastQCFastQC Report
Thu 2 Feb 2017
SRR4062761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927919
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT70690.24143427465035747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT51340.17534638082542583No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA35530.12134898540567549No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG35020.11960713394052226No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA33910.11581604545754169No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA33510.1144498874456568No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC33130.11315203733436616No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC33020.1127763438810978No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC31900.1089511014478201No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA31280.10683355652939854No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG30790.10516001296483952No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG30250.10331569964879493No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC30160.10300831409612082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGC601.13813585E-418.66843
ATCGTTT7650.016.52187229
ACCGTCG6450.016.1269058
CGTTTAT8000.015.99902631
CGTCGTA6650.015.88063210
CATCGTT8000.015.79903928
TCTAGCG7200.015.77681828
CTAGCGG7100.015.77368829
TACCGTC6750.015.6475027
TAACGCA951.4324072E-515.1595614
CCGTCGT7050.014.9808839
TTTTCGG8750.014.81052729
GTACGGA650.00416200114.7700981
TAGCGGC7600.014.73594630
ATACCGT7500.014.7228766
TAAACGC6850.014.71443428
TCGTTTA8500.014.68145930
GTATTAT2450.014.3681911
TAGGACG19600.014.2872854
TTAGGAC11800.014.238613
Produced by FastQC (version 0.11.5)