##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062761_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2927919
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.161467240043184	32.0	32.0	32.0	32.0	32.0
2	31.21451003255213	32.0	32.0	32.0	32.0	32.0
3	31.32501206488294	32.0	32.0	32.0	32.0	32.0
4	31.437382318295008	32.0	32.0	32.0	32.0	32.0
5	31.3438356730497	32.0	32.0	32.0	32.0	32.0
6	34.85766238751823	36.0	36.0	36.0	32.0	36.0
7	34.900108916947495	36.0	36.0	36.0	36.0	36.0
8	34.82849286472747	36.0	36.0	36.0	32.0	36.0
9	34.95629762981831	36.0	36.0	36.0	32.0	36.0
10	34.78093519663624	36.0	36.0	36.0	32.0	36.0
11	34.97449587915513	36.0	36.0	36.0	36.0	36.0
12	34.87005548992305	36.0	36.0	36.0	32.0	36.0
13	34.925054962244516	36.0	36.0	36.0	36.0	36.0
14	34.868618974773554	36.0	36.0	36.0	32.0	36.0
15	34.83798356443604	36.0	36.0	36.0	32.0	36.0
16	34.84936297759603	36.0	36.0	36.0	32.0	36.0
17	34.81889458007547	36.0	36.0	36.0	32.0	36.0
18	34.81652702824088	36.0	36.0	36.0	32.0	36.0
19	34.80501783006975	36.0	36.0	36.0	32.0	36.0
20	34.792931088599104	36.0	36.0	36.0	32.0	36.0
21	34.77680222711079	36.0	36.0	36.0	32.0	36.0
22	34.7589291233808	36.0	36.0	36.0	32.0	36.0
23	34.699666554983246	36.0	36.0	36.0	32.0	36.0
24	34.679011270462055	36.0	36.0	36.0	32.0	36.0
25	34.65497781871698	36.0	36.0	36.0	32.0	36.0
26	34.58466234892427	36.0	36.0	36.0	32.0	36.0
27	34.56225223443681	36.0	36.0	36.0	32.0	36.0
28	34.52710679496256	36.0	36.0	36.0	32.0	36.0
29	34.49084657055062	36.0	36.0	36.0	32.0	36.0
30	34.46329423730643	36.0	36.0	36.0	32.0	36.0
31	34.45870258022848	36.0	36.0	36.0	32.0	36.0
32	34.42223367518022	36.0	36.0	36.0	32.0	36.0
33	34.393673458862764	36.0	36.0	36.0	32.0	36.0
34	34.38359155427455	36.0	36.0	36.0	32.0	36.0
35	34.34725448347444	36.0	36.0	36.0	32.0	36.0
36	34.320956283285156	36.0	36.0	36.0	32.0	36.0
37	34.29899187784908	36.0	36.0	36.0	32.0	36.0
38	33.85906474871744	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	6.0
21	68.0
22	211.0
23	726.0
24	2075.0
25	4988.0
26	10834.0
27	20655.0
28	35862.0
29	56657.0
30	86298.0
31	124401.0
32	179670.0
33	286492.0
34	686398.0
35	1432575.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.26806940745576	18.73964416399648	12.472841281047868	26.519445147499894
2	15.193077404122176	20.841389396359666	37.7250873401894	26.24044585932876
3	17.502225983710616	25.24321881855338	29.09363271320006	28.160922484535945
4	11.737545390708464	16.77427463666941	36.62429062075636	34.863889351865765
5	13.844659477935842	37.525632565961594	33.43951807360594	15.190189882496629
6	33.768603701204135	36.43541882406302	16.89152542835987	12.904452046372977
7	29.792616576329188	30.861228657984707	21.237298960692097	18.108855804994008
8	27.78187444391884	33.76092736832938	19.34938717935144	19.107811008400347
9	27.55058161557187	14.053896569873622	18.528017520364315	39.8675042941902
10	15.566432606287764	27.05052158953399	31.86155019490231	25.521495609275934
11	37.170506811706176	20.889630754496448	22.918353113000613	19.021509320796763
12	25.060239235577335	23.621157624775908	29.21299808156606	22.105605058080698
13	29.74570641565756	19.783702737272087	25.22976247602816	25.24082837104219
14	23.316970175745983	20.407190226232352	25.279524467719224	30.996315130302442
15	24.773636155918247	28.32592021842134	22.309155410378498	24.59128821528191
16	25.211848559914777	25.838573975969947	24.546255921872103	24.403321542243173
17	23.60591259525964	26.38607830339569	25.65914562527174	24.34886347607294
18	24.645524688353742	24.679234637297	27.253827718594675	23.42141295575458
19	25.659043163420847	25.051478541585336	25.558015778441955	23.731462516551858
20	25.77933337636731	24.475369707973478	25.428299075213488	24.316997840445723
21	26.771355569649778	24.44580832832123	24.356188179088655	24.426647922940333
22	25.506784852996272	25.093658670202284	25.039319735279562	24.36023674152188
23	23.651078430267567	24.76883705219383	25.937690088469157	25.642394429069444
24	24.85881610795927	25.278568157110904	25.471333052587863	24.391282682341963
25	24.677774445869044	24.902165975959708	25.683198778107858	24.73686080006339
26	24.030470819518047	25.842749587078316	26.124554119722017	24.00222547368162
27	24.959783654847786	25.389987547448456	25.01968978597049	24.63053901173327
28	23.874977415700364	25.20513716397209	25.834799391649838	25.085086028677704
29	24.24520623692117	25.081124170443243	25.726463061307363	24.947206531328224
30	24.062755834433943	25.19530082628652	26.228184591172095	24.513758748107445
31	24.991265127211513	24.968791827916004	25.015172892419496	25.024770152452984
32	24.342203455765	25.44633236097037	25.021149833721495	25.19031434954314
33	23.74662687048378	25.02429199714883	25.991702639314816	25.237378493052574
34	24.67390662105065	25.15247177261393	26.15369482557407	24.019926780761352
35	25.32320737014924	25.072312451266583	25.73971479402265	23.864765384561526
36	24.119622161678652	25.671782586881676	25.54674497484391	24.661850276595768
37	25.13109998299133	25.539717983905284	24.888811776832547	24.44037025627084
38	24.318764609367207	25.497008793968135	25.52562983364277	24.65859676302189
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	77.0
1	114.0
2	151.0
3	151.0
4	614.5
5	1078.0
6	1078.0
7	1410.0
8	1742.0
9	1929.0
10	2116.0
11	2116.0
12	2693.5
13	3271.0
14	4482.0
15	5693.0
16	5693.0
17	8825.5
18	11958.0
19	11958.0
20	14368.0
21	16778.0
22	19182.5
23	21587.0
24	21587.0
25	27261.0
26	32935.0
27	32935.0
28	42719.0
29	52503.0
30	67448.0
31	82393.0
32	82393.0
33	104775.0
34	127157.0
35	127157.0
36	139002.0
37	150847.0
38	166551.5
39	182256.0
40	182256.0
41	188009.0
42	193762.0
43	211190.0
44	228618.0
45	228618.0
46	235308.0
47	241998.0
48	241998.0
49	253421.5
50	264845.0
51	261251.5
52	257658.0
53	257658.0
54	246178.0
55	234698.0
56	234698.0
57	227391.0
58	220084.0
59	197068.5
60	174053.0
61	174053.0
62	164218.5
63	154384.0
64	127276.5
65	100169.0
66	100169.0
67	83187.5
68	66206.0
69	66206.0
70	53564.0
71	40922.0
72	31781.0
73	22640.0
74	22640.0
75	16712.0
76	10784.0
77	10784.0
78	10748.0
79	10712.0
80	8291.0
81	5870.0
82	5870.0
83	5573.0
84	5276.0
85	5276.0
86	3323.0
87	1370.0
88	1161.5
89	953.0
90	953.0
91	579.0
92	205.0
93	138.5
94	72.0
95	72.0
96	56.5
97	41.0
98	41.0
99	49.0
100	57.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007582176965961148
2	0.0
3	0.0
4	2.3907765207985601E-4
5	1.0246185089136687E-4
6	2.3907765207985601E-4
7	3.0738555267410063E-4
8	0.002185852819015827
9	0.0043033977374374085
10	0.001434465912479136
11	0.0056354017990251784
12	3.415395029712229E-4
13	1.3661580118848917E-4
14	0.0
15	0.0
16	6.830790059424459E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	2.0492370178273373E-4
22	0.0
23	2.0492370178273373E-4
24	0.0
25	3.4153950297122294E-5
26	1.0246185089136687E-4
27	1.7076975148561145E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	3.4153950297122294E-5
38	1.0246185089136687E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2927919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.75072602772234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.47159165376117	31.536548908349904
2	16.013051979248534	14.331914039820562
3	5.968266138110531	8.012527284213505
4	2.8259781525099394	5.05858296253205
5	1.4223989601299614	3.1826693083446522
6	0.8330264502261492	2.236712306874989
7	0.5440525999099016	1.7042724190266627
8	0.357392174510272	1.279484742876889
9	0.25445276645262777	1.0248251434665807
>10	1.1205486710960717	8.777229534230258
>50	0.08040202813133361	2.517402596564846
>100	0.08442401540927957	8.15795668075962
>500	0.01504787565682878	4.614702673887732
>1k	0.00921298509598943	7.14590909231535
>5k	1.5354975159982383E-4	0.41926230673656273
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7069	0.24143427465035747	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5134	0.17534638082542583	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3553	0.12134898540567549	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3502	0.11960713394052226	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3391	0.11581604545754169	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3351	0.1144498874456568	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3313	0.11315203733436616	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3302	0.1127763438810978	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3190	0.1089511014478201	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	3128	0.10683355652939854	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3079	0.10516001296483952	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3025	0.10331569964879493	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3016	0.10300831409612082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.830790059424459E-5
2	0.0	0.0	0.0	0.0	6.830790059424459E-5
3	0.0	0.0	0.0	0.0	6.830790059424459E-5
4	0.0	0.0	0.0	0.0	6.830790059424459E-5
5	0.0	0.0	0.0	0.0	6.830790059424459E-5
6	0.0	0.0	0.0	0.0	6.830790059424459E-5
7	0.0	0.0	0.0	3.4153950297122294E-5	6.830790059424459E-5
8	0.0	0.0	0.0	6.830790059424459E-5	6.830790059424459E-5
9	0.0	0.0	0.0	1.7076975148561145E-4	6.830790059424459E-5
10	0.0	0.0	0.0	1.7076975148561145E-4	6.830790059424459E-5
11	0.0	0.0	0.0	1.7076975148561145E-4	6.830790059424459E-5
12	0.0	0.0	0.0	2.0492370178273373E-4	2.7323160237697835E-4
13	0.0	0.0	0.0	2.0492370178273373E-4	3.0738555267410063E-4
14	0.0	0.0	0.0	2.0492370178273373E-4	3.0738555267410063E-4
15	0.0	0.0	0.0	2.3907765207985604E-4	3.415395029712229E-4
16	0.0	0.0	0.0	2.3907765207985604E-4	3.415395029712229E-4
17	0.0	0.0	0.0	2.3907765207985604E-4	3.415395029712229E-4
18	0.0	0.0	0.0	2.3907765207985604E-4	3.415395029712229E-4
19	0.0	0.0	0.0	3.415395029712229E-4	3.415395029712229E-4
20	0.0	0.0	0.0	4.781553041597121E-4	3.756934532683452E-4
21	0.0	0.0	0.0	5.464632047539567E-4	4.0984740356546747E-4
22	0.0	0.0	3.4153950297122294E-5	8.538487574280573E-4	4.781553041597121E-4
23	0.0	0.0	3.4153950297122294E-5	0.0011953882603992802	4.781553041597121E-4
24	0.0	0.0	3.4153950297122294E-5	0.001878467266341726	4.781553041597121E-4
25	0.0	0.0	3.4153950297122294E-5	0.0021516988687187042	4.781553041597121E-4
26	0.0	0.0	3.4153950297122294E-5	0.0028689318249582726	4.781553041597121E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAACGC	60	1.13813585E-4	18.6684	3
ATCGTTT	765	0.0	16.521872	29
ACCGTCG	645	0.0	16.126905	8
CGTTTAT	800	0.0	15.999026	31
CGTCGTA	665	0.0	15.880632	10
CATCGTT	800	0.0	15.799039	28
TCTAGCG	720	0.0	15.776818	28
CTAGCGG	710	0.0	15.773688	29
TACCGTC	675	0.0	15.647502	7
TAACGCA	95	1.4324072E-5	15.159561	4
CCGTCGT	705	0.0	14.980883	9
TTTTCGG	875	0.0	14.810527	29
GTACGGA	65	0.004162001	14.770098	1
TAGCGGC	760	0.0	14.735946	30
ATACCGT	750	0.0	14.722876	6
TAAACGC	685	0.0	14.714434	28
TCGTTTA	850	0.0	14.681459	30
GTATTAT	245	0.0	14.368191	1
TAGGACG	1960	0.0	14.287285	4
TTAGGAC	1180	0.0	14.23861	3
>>END_MODULE