FastQCFastQC Report
Thu 2 Feb 2017
SRR4062760_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062760_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3348723
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT55520.16579454317362172No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA43730.1305870924528544No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA43600.13019888476891042No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC42100.12571956533878736No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG41980.1253612197843775No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC41630.12431604525068211No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA40380.1205832790589129No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG39310.11738803119875846No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG38910.1161935460173923No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT37550.11213229640074737No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC37320.11144546742146186No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG36770.10980305029708338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34160.10200903448866926No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC34070.10174027532286187No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA33530.10012772032801757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT8950.017.16171629
CATCGTT9150.016.61123828
TCTAGCG8150.016.49001928
ACCGTCG7300.016.2181728
GTCCTAC12650.016.076171
TAGCGGC8550.015.906157530
TACCGTC7250.015.8886697
CTAGCGG8500.015.81149129
CGAGCCG13200.015.75800915
CGTCGTA7450.015.67851810
CAAGACG11250.015.64020254
AGGCCCG13350.015.58118710
GTCCTAT12050.015.5477721
ATACCGT8100.015.2064356
ACGTTTT6550.015.14477529
CGTTTAT10400.015.07666231
CCGTCGT7700.014.9616759
ACGCTTA1308.3238774E-814.76941617
CGCAAGA11500.014.7513172
GGCATCG10200.014.744843526
Produced by FastQC (version 0.11.5)