FastQCFastQC Report
Thu 2 Feb 2017
SRR4062760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3348723
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT56470.16863144547936632No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC45960.1372463473389707No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC45430.13566365447366055No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA44960.13426013438555534No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA43570.13010929838030796No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC42940.12822798421965625No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG42270.12622722154086796No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA41800.12482370145276275No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG41530.12401742395534059No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC40250.12019507137496892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT39040.1165817537013363No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG38360.11455112889301383No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT37780.11281912538003293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT8350.018.3943528
ATCGTTT8900.017.4373929
CGGCATC9100.017.4057825
TGTAGGA15950.016.351312
CTAGCGG9700.016.32913629
GGCATCG9750.016.24539626
GCATCGT9850.016.24289727
ACCGTCG9300.016.1739468
CGTTTAT9700.016.16419431
TACCGTC9050.016.090297
TAGGACG10250.015.9226354
TCTAGCG10100.015.68243728
ATACCGT9850.015.5957426
GTAGGAC15600.015.5904133
GGACGTG10750.015.183186
TCGCGTA4450.015.1024499
TTAGGAC7650.015.0594693
TAGCGGC10550.015.01351730
TTTTCGG12650.014.79772929
CGTCGTA10100.014.73352610
Produced by FastQC (version 0.11.5)