##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062760_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3348723
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.201740782979066	32.0	32.0	32.0	32.0	32.0
2	31.251998746985045	32.0	32.0	32.0	32.0	32.0
3	31.36552082689431	32.0	32.0	32.0	32.0	32.0
4	31.47473947531641	32.0	32.0	32.0	32.0	32.0
5	31.389473240993656	32.0	32.0	32.0	32.0	32.0
6	34.92372614874387	36.0	36.0	36.0	36.0	36.0
7	34.95396513835274	36.0	36.0	36.0	36.0	36.0
8	34.884332027462406	36.0	36.0	36.0	32.0	36.0
9	35.00808039363065	36.0	36.0	36.0	36.0	36.0
10	34.84306584928046	36.0	36.0	36.0	32.0	36.0
11	35.03941114269529	36.0	36.0	36.0	36.0	36.0
12	34.924818505442225	36.0	36.0	36.0	32.0	36.0
13	34.98447079677835	36.0	36.0	36.0	36.0	36.0
14	34.92993956203603	36.0	36.0	36.0	32.0	36.0
15	34.89382191360707	36.0	36.0	36.0	32.0	36.0
16	34.90665008721235	36.0	36.0	36.0	32.0	36.0
17	34.87766202220966	36.0	36.0	36.0	32.0	36.0
18	34.881553953551844	36.0	36.0	36.0	32.0	36.0
19	34.87069996533007	36.0	36.0	36.0	32.0	36.0
20	34.856465285423724	36.0	36.0	36.0	32.0	36.0
21	34.84761205988074	36.0	36.0	36.0	32.0	36.0
22	34.82597903738231	36.0	36.0	36.0	32.0	36.0
23	34.76311089331664	36.0	36.0	36.0	32.0	36.0
24	34.740200368916746	36.0	36.0	36.0	32.0	36.0
25	34.72118924139142	36.0	36.0	36.0	32.0	36.0
26	34.65302833348712	36.0	36.0	36.0	32.0	36.0
27	34.63636466796447	36.0	36.0	36.0	32.0	36.0
28	34.59856876785569	36.0	36.0	36.0	32.0	36.0
29	34.556931403403624	36.0	36.0	36.0	32.0	36.0
30	34.54008468302693	36.0	36.0	36.0	32.0	36.0
31	34.53432816031663	36.0	36.0	36.0	32.0	36.0
32	34.51106795037989	36.0	36.0	36.0	32.0	36.0
33	34.47557561494337	36.0	36.0	36.0	32.0	36.0
34	34.46876734803087	36.0	36.0	36.0	32.0	36.0
35	34.43149821588707	36.0	36.0	36.0	32.0	36.0
36	34.40274008928179	36.0	36.0	36.0	32.0	36.0
37	34.3866052820732	36.0	36.0	36.0	32.0	36.0
38	33.94283492543277	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	15.0
21	61.0
22	209.0
23	727.0
24	2081.0
25	5058.0
26	11167.0
27	21268.0
28	37870.0
29	59831.0
30	91867.0
31	134269.0
32	194908.0
33	316783.0
34	778422.0
35	1694185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.71953855264915	18.3556966104322	12.21671348973143	26.70805134718723
2	15.497912337900846	20.633304287426665	37.54067969810573	26.328103676566762
3	17.70433087478421	25.02180682009232	28.69162364280354	28.582238662319938
4	11.682444351075352	16.529076658314214	36.73597977971862	35.05249921089181
5	13.815738983019468	37.386855921237895	33.63207461245605	15.165330483286585
6	33.966440908765136	36.454227286843924	16.795521871043263	12.783809933347667
7	30.01236594476744	30.64431021708937	21.128326016592048	18.214997821551147
8	27.888525690682144	33.57625738880729	19.339159767858412	19.196057152652145
9	27.5043152104449	14.201641273121465	18.199556832366767	40.09448668406687
10	15.178165888007452	27.0492921890701	31.899853824090208	25.872688098832235
11	37.76387525115334	21.009379576605053	22.42814207454446	18.798603097697153
12	24.748119500309222	23.637453336522622	29.17152301288466	22.442904150283496
13	29.584071052798965	19.6258213210357	25.249371327142512	25.54073629902282
14	23.402771743139102	20.115160316335512	25.112080037674062	31.369987902851328
15	25.072990510113858	27.84888448522019	22.160865500072717	24.917259504593243
16	25.691510281089407	25.80621078913412	24.193804141939566	24.308474787836907
17	23.82096100513539	26.094842720643065	25.500018962452252	24.58417731176929
18	24.89414621633381	24.789598900834736	26.77486313439481	23.541391748436645
19	25.65177830474482	25.205578365245497	25.211819550318136	23.930823779691544
20	25.64440235874989	24.443287784627156	25.28602694221051	24.62628291441245
21	26.846520622674298	24.482600351119547	24.14835293636339	24.522526089842767
22	25.43509161709549	24.86287297833202	25.035326930887024	24.666708473685468
23	23.93244935299101	24.467788708332176	25.56719483909808	26.032567099578735
24	24.674331080832903	25.42369135936296	25.192170269084663	24.709807290719475
25	24.708694665424403	24.810345445423923	25.31095463341217	25.170005255739508
26	24.17440096514489	25.757423732052843	25.91470770921427	24.153467593587997
27	25.281786519816656	25.069884848642303	24.858819317094905	24.789509314446136
28	24.09867880980302	25.132864079829837	25.663633570169882	25.104823540197263
29	24.28221145791993	25.030227940620946	25.698303502559032	24.98925709890009
30	24.23347646252019	25.212237620131617	26.004778537968054	24.549507379380138
31	25.01658094742384	24.946345218759507	24.752420549564714	25.284653284251934
32	24.429491480782374	25.350290244968004	24.85849083367003	25.36172744057959
33	23.987412515158763	24.7105239818283	25.73186853615542	25.570194966857517
34	24.758542286119216	25.157321163918304	25.837013094245183	24.247123455717297
35	25.624902388164084	24.872257275385273	25.49912309856623	24.003717237884413
36	24.234730671960627	25.555263902090434	25.294895994682154	24.915109431266785
37	25.48935818220856	25.35930860808732	24.789180831021255	24.362152378682858
38	24.494725000977986	25.13425274903463	25.479817518389858	24.891204731597526
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	63.0
2	99.0
3	99.0
4	409.0
5	719.0
6	719.0
7	1000.5
8	1282.0
9	1513.0
10	1744.0
11	1744.0
12	2262.0
13	2780.0
14	3906.0
15	5032.0
16	5032.0
17	7754.5
18	10477.0
19	10477.0
20	13207.5
21	15938.0
22	19541.5
23	23145.0
24	23145.0
25	30232.0
26	37319.0
27	37319.0
28	49082.0
29	60845.0
30	74893.5
31	88942.0
32	88942.0
33	112892.5
34	136843.0
35	136843.0
36	150457.5
37	164072.0
38	185111.5
39	206151.0
40	206151.0
41	214366.5
42	222582.0
43	246059.0
44	269536.0
45	269536.0
46	277290.0
47	285044.0
48	285044.0
49	296614.0
50	308184.0
51	303454.0
52	298724.0
53	298724.0
54	282504.0
55	266284.0
56	266284.0
57	257534.0
58	248784.0
59	222500.5
60	196217.0
61	196217.0
62	187343.5
63	178470.0
64	147996.5
65	117523.0
66	117523.0
67	98408.0
68	79293.0
69	79293.0
70	64520.0
71	49747.0
72	39026.0
73	28305.0
74	28305.0
75	21368.0
76	14431.0
77	14431.0
78	13831.0
79	13231.0
80	10211.0
81	7191.0
82	7191.0
83	6807.0
84	6423.0
85	6423.0
86	4101.0
87	1779.0
88	1470.5
89	1162.0
90	1162.0
91	695.5
92	229.0
93	156.5
94	84.0
95	84.0
96	61.0
97	38.0
98	38.0
99	42.5
100	47.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009018363119314437
2	2.9862129534153765E-5
3	0.0
4	1.7917277720492258E-4
5	1.7917277720492258E-4
6	8.958638860246129E-5
7	2.986212953415377E-4
8	0.00229938397412984
9	0.003075799342017838
10	5.375183316147677E-4
11	0.005106424150340294
12	3.5834555440984517E-4
13	5.972425906830753E-5
14	0.0
15	0.0
16	5.972425906830753E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.7917277720492258E-4
22	8.958638860246129E-5
23	1.7917277720492258E-4
24	0.0
25	8.958638860246129E-5
26	8.958638860246129E-5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	5.972425906830753E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3348723.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.55486833630315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.24807785388387	29.46842835614098
2	16.076826516997546	13.682944713928356
3	6.332434794787137	8.084277868211744
4	3.019683906746579	5.140090042754167
5	1.6120105000089375	3.429944729230927
6	0.9846979086575565	2.5142213912373235
7	0.6278343402600688	1.8702185380743883
8	0.40956642919976793	1.394323637565276
9	0.26964113502044373	1.032708869896169
>10	1.2225662583591286	8.971752601406688
>50	0.08537473551516127	2.5369506953943937
>100	0.08268459017392683	7.580320338026464
>500	0.017486273530802232	5.229368536292102
>1k	0.011043962229443562	8.894324989345455
>5k	7.079462967592026E-5	0.17012469249550788
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5647	0.16863144547936632	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	4596	0.1372463473389707	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4543	0.13566365447366055	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	4496	0.13426013438555534	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	4357	0.13010929838030796	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	4294	0.12822798421965625	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	4227	0.12622722154086796	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	4180	0.12482370145276275	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	4153	0.12401742395534059	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	4025	0.12019507137496892	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3904	0.1165817537013363	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3836	0.11455112889301383	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3778	0.11281912538003293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.9862129534153765E-5	0.0
5	0.0	0.0	0.0	2.9862129534153765E-5	0.0
6	0.0	0.0	0.0	2.9862129534153765E-5	0.0
7	0.0	0.0	0.0	2.9862129534153765E-5	0.0
8	0.0	0.0	0.0	5.972425906830753E-5	0.0
9	0.0	0.0	0.0	2.9862129534153763E-4	0.0
10	0.0	0.0	0.0	3.5834555440984517E-4	0.0
11	0.0	0.0	0.0	4.180698134781527E-4	0.0
12	0.0	0.0	0.0	4.180698134781527E-4	0.0
13	0.0	0.0	0.0	4.180698134781527E-4	0.0
14	0.0	0.0	0.0	4.180698134781527E-4	0.0
15	0.0	0.0	0.0	5.375183316147678E-4	0.0
16	0.0	0.0	0.0	5.972425906830753E-4	0.0
17	0.0	0.0	0.0	6.27104720217229E-4	0.0
18	0.0	0.0	0.0	6.868289792855366E-4	0.0
19	0.0	0.0	0.0	8.958638860246129E-4	0.0
20	0.0	0.0	0.0	9.555881450929205E-4	0.0
21	0.0	0.0	0.0	0.0010451745336953818	0.0
22	0.0	0.0	0.0	0.0011646230518319969	0.0
23	0.0	0.0	0.0	0.0014632443471735345	0.0
24	0.0	0.0	0.0	0.0017618656425150722	0.0
25	0.0	0.0	0.0	0.0019709005492541485	0.0
26	0.0	0.0	0.0	0.002568143139937224	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGTT	835	0.0	18.39435	28
ATCGTTT	890	0.0	17.43739	29
CGGCATC	910	0.0	17.40578	25
TGTAGGA	1595	0.0	16.35131	2
CTAGCGG	970	0.0	16.329136	29
GGCATCG	975	0.0	16.245396	26
GCATCGT	985	0.0	16.242897	27
ACCGTCG	930	0.0	16.173946	8
CGTTTAT	970	0.0	16.164194	31
TACCGTC	905	0.0	16.09029	7
TAGGACG	1025	0.0	15.922635	4
TCTAGCG	1010	0.0	15.682437	28
ATACCGT	985	0.0	15.595742	6
GTAGGAC	1560	0.0	15.590413	3
GGACGTG	1075	0.0	15.18318	6
TCGCGTA	445	0.0	15.102449	9
TTAGGAC	765	0.0	15.059469	3
TAGCGGC	1055	0.0	15.013517	30
TTTTCGG	1265	0.0	14.797729	29
CGTCGTA	1010	0.0	14.733526	10
>>END_MODULE