FastQCFastQC Report
Thu 2 Feb 2017
SRR4062759_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062759_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838207
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA22790.27188987923030944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT21620.25793151333739756No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC17510.20889827930332247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT14610.1743006202525152No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11470.13683970665957215No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11000.1312324998478896No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10940.13051668621235565No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10810.12896575666869878No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10110.12061459758746945No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10090.12037599304229148No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9850.11751273850015569No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9790.11679692486462173No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9450.11274064759659608No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT8940.10665623169455755No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG8750.10438948851536672No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT8690.1036736748798328No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG8680.10355437260724379No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA8450.10081042033769701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT8390.10009460670216307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT453.5364224E-421.33265519
CTAATTC604.73645E-621.3237483
TACCGTC2350.018.3802017
GTACATA701.7794746E-518.2938691
TTATACT802.807963E-617.990844
TCGATAT450.00883778217.78145810
AACGTTC450.00884082217.78039721
TATTAAG904.3701584E-717.7750912
ATACCGT2250.017.769796
TATACCG450.00887431617.768735
CGTTGTA550.001362880917.45711720
TAAGGTG550.001368889417.4456635
CGTCGTA2300.017.39490710
ACCGTCG2300.017.3886788
GTATTAC753.2196578E-517.0742781
ATAATAC753.24449E-517.0589983
GTATTAA1051.18190655E-716.769381
GTCGTAG2400.016.67011811
CCGTCGT2400.016.0033139
AGGCCCG3100.016.00331310
Produced by FastQC (version 0.11.5)