##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062759_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	838207
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24226473890101	32.0	32.0	32.0	32.0	32.0
2	30.90806686176565	32.0	32.0	32.0	32.0	32.0
3	30.94873223440033	32.0	32.0	32.0	32.0	32.0
4	31.01286436405327	32.0	32.0	32.0	32.0	32.0
5	30.94621734249416	32.0	32.0	32.0	32.0	32.0
6	34.599668101077654	36.0	36.0	36.0	32.0	36.0
7	34.52936685090914	36.0	36.0	36.0	32.0	36.0
8	34.51794723737692	36.0	36.0	36.0	32.0	36.0
9	34.623969974004034	36.0	36.0	36.0	32.0	36.0
10	34.368706059481724	36.0	36.0	36.0	32.0	36.0
11	34.620445784871755	36.0	36.0	36.0	32.0	36.0
12	34.45873155437738	36.0	36.0	36.0	32.0	36.0
13	34.53556937606105	36.0	36.0	36.0	32.0	36.0
14	34.43069671334169	36.0	36.0	36.0	32.0	36.0
15	34.38306170194236	36.0	36.0	36.0	32.0	36.0
16	34.39896827394665	36.0	36.0	36.0	32.0	36.0
17	34.31872437238057	36.0	36.0	36.0	32.0	36.0
18	34.340315697673724	36.0	36.0	36.0	32.0	36.0
19	34.3391811330614	36.0	36.0	36.0	32.0	36.0
20	34.309875722822646	36.0	36.0	36.0	32.0	36.0
21	34.27775716499624	36.0	36.0	36.0	32.0	36.0
22	34.25934047317667	36.0	36.0	36.0	32.0	36.0
23	34.20056740160843	36.0	36.0	36.0	32.0	36.0
24	34.18843197444068	36.0	36.0	36.0	32.0	36.0
25	34.165400670717375	36.0	36.0	36.0	32.0	36.0
26	34.10622435746779	36.0	36.0	36.0	32.0	36.0
27	34.09821559590889	36.0	36.0	36.0	32.0	36.0
28	34.07776957243259	36.0	36.0	36.0	32.0	36.0
29	34.04397243163085	36.0	36.0	36.0	32.0	36.0
30	34.007066273605446	36.0	36.0	36.0	32.0	36.0
31	34.03856803868257	36.0	36.0	36.0	32.0	36.0
32	33.980692120204196	36.0	36.0	36.0	32.0	36.0
33	33.941059905250135	36.0	36.0	36.0	32.0	36.0
34	33.94033693347824	36.0	36.0	36.0	32.0	36.0
35	33.88099001797885	36.0	36.0	36.0	32.0	36.0
36	33.84337759049972	36.0	36.0	36.0	32.0	36.0
37	33.83314145551159	36.0	36.0	36.0	32.0	36.0
38	33.21564601584095	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	4.0
10	5.0
11	10.0
12	8.0
13	7.0
14	114.0
15	245.0
16	292.0
17	343.0
18	434.0
19	561.0
20	771.0
21	1144.0
22	1586.0
23	2408.0
24	3486.0
25	5172.0
26	7665.0
27	11279.0
28	15550.0
29	21863.0
30	29328.0
31	39351.0
32	53555.0
33	80444.0
34	177612.0
35	384967.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.62830336293545	16.96015957669177	11.443536009268826	25.968001051103958
2	16.684252742950452	19.864666787620674	37.23883483093494	26.21224563849393
3	18.729164827245544	24.36638405203991	28.541770569102976	28.362680551611568
4	12.505890676207873	15.66746879283789	35.95597174384297	35.870668787111256
5	14.682411385254479	36.58213305305253	33.3662209931437	15.369234568549297
6	33.99558103855138	35.33439273877782	17.1426833021954	13.527342920475396
7	29.982569937974752	30.5498522441354	21.276605898065753	18.1909719198241
8	28.488802831772254	32.261158693061226	19.77265650445059	19.477381970715925
9	27.827440781193147	14.219910403779505	18.34396120280838	39.608687612218965
10	16.084805434496023	26.347021776399693	31.376483683507892	26.19168910559639
11	37.542681253653704	20.946205514262875	22.109545568427205	19.40156766365621
12	24.94804255857292	23.6909230170869	28.597351900413514	22.763682523926672
13	29.921487208858345	19.158672203130966	25.010142229858722	25.909698358151967
14	24.230655422399764	19.59513957495144	24.871974762443386	31.302230240205414
15	25.65671946357419	26.485725980350622	22.647498600820295	25.21005595525489
16	26.175962085715206	25.243974174950274	23.76181391025664	24.818249829077878
17	24.25184391433689	25.3280622223389	25.409443848467888	25.010650014856324
18	25.51977321590066	24.112155999971367	26.191248863573996	24.17682192055398
19	25.66780094592208	25.25020164080733	25.200924922803786	23.881072490466803
20	25.81778223732955	23.60004342892868	25.395185354428975	25.186988979312797
21	27.3965836297234	24.115632095315704	23.83393724922179	24.653847025739108
22	25.92102971109769	24.217372318162404	24.55848559545087	25.303112375289032
23	24.319567787883887	23.845523304303626	25.238185577736495	26.596723330075996
24	24.70304253874616	25.17769719705566	24.89288053808513	25.226379726113045
25	25.310033351033045	23.97444081832338	24.809350221644163	25.90617560899941
26	24.83019579391521	24.791772073353506	25.491155384571325	24.88687674815996
27	25.77267802854947	24.736554495516177	24.362146889959003	25.128620585975348
28	24.700172284608445	24.15301354891976	25.60466647895121	25.542147687520583
29	24.508448164980535	24.89479317178751	25.644097261579912	24.952661401652048
30	24.869329509160952	24.723894833668783	25.79049532497313	24.61628033219714
31	25.07182502374282	24.633835551716405	24.500446221908305	25.79389320263247
32	24.626730331903968	24.84149312639745	24.42688162036142	26.104894921337163
33	24.307204406020098	24.329635400882445	25.116390614299043	26.246769578798414
34	25.098467827479354	24.685982787072046	25.471219712417863	24.744329673030734
35	26.144615979399894	24.279700353437843	25.250878821651114	24.324804845511146
36	24.891267383794812	25.22012015774433	24.68328828909452	25.20532416936634
37	25.814926883280513	24.99832957886297	24.764351303883014	24.422392233973504
38	24.645170065429973	24.895722473835168	25.013840037797618	25.44526742293724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	303.0
1	259.0
2	215.0
3	215.0
4	475.5
5	736.0
6	736.0
7	796.0
8	856.0
9	808.5
10	761.0
11	761.0
12	1000.5
13	1240.0
14	1556.0
15	1872.0
16	1872.0
17	2702.5
18	3533.0
19	3533.0
20	4257.0
21	4981.0
22	5268.5
23	5556.0
24	5556.0
25	6527.5
26	7499.0
27	7499.0
28	9604.5
29	11710.0
30	14468.0
31	17226.0
32	17226.0
33	21906.5
34	26587.0
35	26587.0
36	29924.0
37	33261.0
38	38507.0
39	43753.0
40	43753.0
41	47233.5
42	50714.0
43	58305.5
44	65897.0
45	65897.0
46	70670.5
47	75444.0
48	75444.0
49	78328.0
50	81212.0
51	80037.0
52	78862.0
53	78862.0
54	75085.0
55	71308.0
56	71308.0
57	69443.0
58	67578.0
59	60510.0
60	53442.0
61	53442.0
62	50934.5
63	48427.0
64	39808.5
65	31190.0
66	31190.0
67	25925.5
68	20661.0
69	20661.0
70	16756.0
71	12851.0
72	10010.5
73	7170.0
74	7170.0
75	5409.5
76	3649.0
77	3649.0
78	3523.5
79	3398.0
80	2696.5
81	1995.0
82	1995.0
83	2091.5
84	2188.0
85	2188.0
86	1461.5
87	735.0
88	631.0
89	527.0
90	527.0
91	383.0
92	239.0
93	196.5
94	154.0
95	154.0
96	137.5
97	121.0
98	121.0
99	238.5
100	356.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11834785440827862
2	0.05046486130514301
3	0.009663484079708235
4	0.0021474409066018297
5	0.0
6	1.1930227258899055E-4
7	0.0
8	4.772090903559622E-4
9	2.386045451779811E-4
10	7.158136355339433E-4
11	0.0020281386340128393
12	0.0025053477243688017
13	0.0151513886188018
14	0.01085650680559814
15	0.02576929087922196
16	0.012646040894432999
17	0.0214744090660183
18	0.0063230204472164995
19	0.009902088624886216
20	0.006680927264983471
21	0.007873949990873376
22	0.008231856808640347
23	0.011930227258899055
24	0.014793481801034828
25	0.018133945433526564
26	0.021832315883785272
27	0.009902088624886216
28	0.007038834082750442
29	0.011453018168543093
30	0.0035790681776697165
31	0.007038834082750442
32	0.00870906589899631
33	0.009663484079708235
34	0.013839063620322903
35	0.01825324770611555
36	0.016940922707636658
37	0.011333715895954102
38	0.007038834082750442
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	838207.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.64731944761442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.82639699367031	47.74719766189044
2	10.931329936443705	12.603237376668819
3	2.822756283810237	4.881729996467091
4	1.1043877760126413	2.5465997967136467
5	0.5866324082521234	1.6908892918416758
6	0.3497203564513218	1.209626466340976
7	0.23383065656219756	0.9435797388840496
8	0.1615752549781702	0.7451504270845064
9	0.11289276011213828	0.5857168504956606
>10	0.6827830807442726	7.501579460320265
>50	0.08300349327083846	3.3451093024256986
>100	0.09468070490545807	11.279849898612127
>500	0.007924816706045857	3.2252015257124422
>1k	0.0020854780805383832	1.6945322065425454
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2279	0.27188987923030944	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2162	0.25793151333739756	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1751	0.20889827930332247	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1461	0.1743006202525152	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1147	0.13683970665957215	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1100	0.1312324998478896	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1094	0.13051668621235565	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1081	0.12896575666869878	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1011	0.12061459758746945	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1009	0.12037599304229148	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	985	0.11751273850015569	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	979	0.11679692486462173	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	945	0.11274064759659608	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	894	0.10665623169455755	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	875	0.10438948851536672	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	869	0.1036736748798328	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	868	0.10355437260724379	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	845	0.10081042033769701	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	839	0.10009460670216307	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1930227258899054E-4
2	0.0	0.0	0.0	1.1930227258899054E-4	1.1930227258899054E-4
3	0.0	0.0	0.0	1.1930227258899054E-4	1.1930227258899054E-4
4	0.0	0.0	0.0	1.1930227258899054E-4	1.1930227258899054E-4
5	0.0	0.0	0.0	1.1930227258899054E-4	1.1930227258899054E-4
6	0.0	0.0	0.0	1.1930227258899054E-4	1.1930227258899054E-4
7	0.0	0.0	0.0	1.1930227258899054E-4	3.5790681776697166E-4
8	0.0	0.0	0.0	1.1930227258899054E-4	3.5790681776697166E-4
9	0.0	0.0	0.0	1.1930227258899054E-4	3.5790681776697166E-4
10	0.0	0.0	0.0	1.1930227258899054E-4	3.5790681776697166E-4
11	0.0	0.0	0.0	1.1930227258899054E-4	3.5790681776697166E-4
12	0.0	0.0	0.0	1.1930227258899054E-4	3.5790681776697166E-4
13	0.0	0.0	0.0	1.1930227258899054E-4	4.7720909035596216E-4
14	0.0	0.0	0.0	1.1930227258899054E-4	4.7720909035596216E-4
15	0.0	0.0	0.0	2.3860454517798108E-4	4.7720909035596216E-4
16	0.0	0.0	0.0	3.5790681776697166E-4	4.7720909035596216E-4
17	0.0	0.0	0.0	3.5790681776697166E-4	4.7720909035596216E-4
18	0.0	0.0	0.0	5.965113629449527E-4	4.7720909035596216E-4
19	0.0	0.0	0.0	5.965113629449527E-4	4.7720909035596216E-4
20	0.0	0.0	0.0	8.351159081229338E-4	4.7720909035596216E-4
21	0.0	0.0	0.0	0.001312324998478896	4.7720909035596216E-4
22	0.0	0.0	0.0	0.001312324998478896	4.7720909035596216E-4
23	0.0	0.0	0.0	0.0020281386340128393	4.7720909035596216E-4
24	0.0	0.0	0.0	0.003101859087313754	4.7720909035596216E-4
25	0.0	0.0	0.0	0.0033404636324917353	4.7720909035596216E-4
26	0.0	0.0	0.0	0.0040562772680256785	4.7720909035596216E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGT	45	3.5364224E-4	21.332655	19
CTAATTC	60	4.73645E-6	21.323748	3
TACCGTC	235	0.0	18.380201	7
GTACATA	70	1.7794746E-5	18.293869	1
TTATACT	80	2.807963E-6	17.99084	4
TCGATAT	45	0.008837782	17.781458	10
AACGTTC	45	0.008840822	17.780397	21
TATTAAG	90	4.3701584E-7	17.775091	2
ATACCGT	225	0.0	17.76979	6
TATACCG	45	0.008874316	17.76873	5
CGTTGTA	55	0.0013628809	17.457117	20
TAAGGTG	55	0.0013688894	17.445663	5
CGTCGTA	230	0.0	17.394907	10
ACCGTCG	230	0.0	17.388678	8
GTATTAC	75	3.2196578E-5	17.074278	1
ATAATAC	75	3.24449E-5	17.058998	3
GTATTAA	105	1.18190655E-7	16.76938	1
GTCGTAG	240	0.0	16.670118	11
CCGTCGT	240	0.0	16.003313	9
AGGCCCG	310	0.0	16.003313	10
>>END_MODULE