FastQCFastQC Report
Thu 2 Feb 2017
SRR4062759_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062759_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838207
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23400.27916731785823784No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23210.27690057467904705No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC21870.2609140701521223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17220.20543851339824173No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12100.14435574983267857No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11210.1337378475722584No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11080.13218691802860152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11000.1312324998478896No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10610.12657971121691897No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10250.12228482940371531No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10230.12204622485853733No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10130.12085320213264743No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10100.12049529531488044No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9660.11524599532096488No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9640.11500739077578688No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9340.11142832259811716No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT8850.10558251124125663No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG8660.10331576806206581No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8590.10248065215394289No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG8570.10224204760876489No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC8460.10092972261028602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATC601.7581442E-724.0056841
TTGGCGT555.8282858E-520.3648134
ACCGTCG2400.020.002358
ATACCGT2400.020.002356
TAGGACA902.0350853E-819.5566844
TACCGTC2400.019.3356047
CTATACT852.392626E-718.8246174
ATAAGAC601.1372111E-418.6677463
CGTCGTA2650.018.11317410
TAAGACA1159.385985E-1018.0880014
GTCGTAG2600.017.8461211
TAGCGTA1800.017.7798657
TCTAGCG2250.017.77668428
ATCGTTT2250.017.77668429
CCCTAGT550.001362065117.4586771
CATCGTT2400.017.33226828
CCCTAGA753.225443E-517.070711
CTAGCGG2350.017.0202329
CGTATAT1800.016.88885710
GCATCGT2750.016.28990727
Produced by FastQC (version 0.11.5)