FastQCFastQC Report
Thu 2 Feb 2017
SRR4062758_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062758_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547625
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31880.20599305387286973No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA28640.18505774977788547No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG28540.18441159841692917No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA27690.17891931184880058No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG27400.1770454729020273No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG26280.1698085776593167No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC25010.16160245537517162No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24280.15688555044019062No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24130.15591632339875616No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23260.1502948065584363No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22790.14725789516194168No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT22090.14273483563524755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21910.1415717631855262No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA21800.14086099668847427No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21240.13724254906711897No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20290.13110411113803408No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA20180.13039334464098215No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT20170.1303287295048865No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT20060.12961796300783457No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC19780.12780873919715693No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT19210.124125676439706No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT18850.1217995315402633No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18500.11953800177691624No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC18220.11772877796623858No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT17610.11378725466440513No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT17530.11327033357564009No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG16640.107519586463129No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG16600.10726112591874647No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16580.1071318956465552No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG15970.10319037234472175No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15710.10151037880623537No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT15710.10151037880623537No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA15500.10015346094822712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC756.4137566E-821.3308773
CGTATAC400.004487156419.99773
TTAGGAC1456.91216E-1116.5498183
GATATAA805.5788296E-516.0147171
GTACTAA703.6716383E-416.0147171
CCCTATA703.688854E-416.004882
TATAGGG703.688854E-416.004882
ACCGTCG4500.015.9991938
TAGGACG600.00244482315.9976424
GTTCAAA6850.015.8978211
ATCGTTT4700.015.31837629
TCTAGCG5800.015.17164728
GTATTAG4600.014.9702791
CTAGCGG5950.014.78916929
CTAAGGT1308.330244E-814.7670544
TACCGTC5000.014.7192567
TAGCGGC6000.014.666430
CATCGTT4950.014.54472128
TAGACAG1654.8567017E-1014.542845
CGAGCCG6950.014.50380215
Produced by FastQC (version 0.11.5)